Microchip‐Based Capillary Electrophoresis for DNA Analysis in Modern Biotechnology: A Review

Kailasa, Suresh Kumar; Kang, Seong Ho

doi:10.1080/15422110903095136

Cited by 22 publications

(8 citation statements)

References 149 publications

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“…Following purification, nucleic acid targets are amplified for subsequent detection by one of several microchip methods that include electrochemical 40 and optical 41,42 systems as well as electrophoresis. 43 Detection can take place either directly in the amplification chamber as in real-time methods or in separate channels and chambers down-stream from the amplification chamber.…”

Section: Designmentioning

confidence: 99%

Miniaturized isothermal nucleic acid amplification, a review

2011

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Micro-Total Analysis Systems (µTAS) for use in on-site rapid detection of DNA or RNA are increasingly being developed. Here, amplification of the target sequence is key to increasing sensitivity, enabling single-cell and few-copy nucleic acid detection. The several advantages to miniaturizing amplification reactions and coupling them with sample preparation and detection on the same chip are well known and include fewer manual steps, preventing contamination, and significantly reducing the volume of expensive reagents. To-date, the majority of miniaturized systems for nucleic acid analysis have used the polymerase chain reaction (PCR) for amplification and those systems are covered in previous reviews. This review provides a thorough overview of miniaturized analysis systems using alternatives to PCR, specifically isothermal amplification reactions. With no need for thermal cycling, isothermal microsystems can be designed to be simple and low-energy consuming and therefore may outperform PCR in portable, battery-operated detection systems in the future. The main isothermal methods as miniaturized systems reviewed here include nucleic acid sequence-based amplification (NASBA), loop-mediated isothermal amplification (LAMP), helicase-dependent amplification (HDA), rolling circle amplification (RCA), and strand displacement amplification (SDA). Also, important design criteria for the miniaturized devices are discussed. Finally, the potential of miniaturization of some new isothermal methods such as the exponential amplification reaction (EXPAR), isothermal and chimeric primer-initiated amplification of nucleic acids (ICANs), signal-mediated amplification of RNA technology (SMART) and others is presented.

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Section: Designmentioning

confidence: 99%

Miniaturized isothermal nucleic acid amplification, a review

2011

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“…ME offers advantages for POCT applications such as rapid performance, low sample and reagent consumption, and miniaturized size. [194][195][196][197] ME is a downscale version of classical electrophoresis that utilizes microfluidic channels etched onto a small chip, which is often fabricated from glass, silicon, quartz, or polymer substrates by photolithography, thermal bonding, or mass-replication processes. [198,199] Figure 5A shows the general schematic representing a setup of an ME and how it works.…”

Section: Electrophoresismentioning

confidence: 99%

Point‐of‐Care Testing (POCT) Devices for DNA Detection: A Comprehensive Review

Mahardika,

Naorungroj,

Khamcharoen

et al. 2023

Advanced NanoBiomed Research

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DNA chips play a crucial role in point‐of‐care diagnostics by enabling rapid and accurate detection of genetic information. These chips offer high sensitivity and selectivity, allowing for the identification of specific DNA sequences associated with diseases and pathogens. Integration into lab‐on‐chip platforms streamlines and miniaturizes diagnostic workflows, paving the way for cost‐effective, portable, and user‐friendly testing devices that can revolutionize healthcare delivery. In this review, a comprehensive description of the platforms utilized in DNA analysis, including microfluidic devices and integrated DNA chips, is provided. It explores the selection and immobilization of DNA probes for improved selectivity. Additionally, it covers diverse detection techniques such as optical detection (colorimetry and fluorescence) and electrochemical techniques. In these discussions, it is aimed to provide a thorough understanding of the current state of the art in DNA biosensor‐integrated lab‐on‐chip technology for point‐of‐care testing. The continued advancements in DNA chip technology hold immense promise for the development of next‐generation point‐of‐care diagnostics, where integrated sample preparation and rapid results generation can further enhance patient outcomes and contribute to the effective management of diseases.

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“…Thus far, various molecular biology methods (PCR technologies (multiplex PCR, real-time PCR and RT-PCR), isothermal amplification technology (RT-LAMP)) and electrochemical sensors have been applied for the detection of various pathogenic species including viral species. [28][29][30][31][32][33] Researchers are seriously focusing their efforts on the design and development of effective drug molecules to suppress SARS-CoV19 and to stop its mutation. Unfortunately, there are no suitable medicines for the treatment of COVID-19 disease and these are under progress, and it will take another 6 to 12 months for the development of medicines or vaccines for the complete treatment of COVID-19.…”

Section: Polymerase Chain Reaction Technology For Diagnosis Of Covid-19mentioning

confidence: 99%