Microchip-based detection of magnetically labeled cancer biomarkers

Abstract

Micro-magnetic sensing and actuation have emerged as powerful tools for the diagnosis and monitoring of cancer. These technologies can be miniaturized and integrated onto compact, microfluidic platforms, enabling molecular diagnostics to be performed in practical clinical settings. Molecular targets tagged with magnetic nanoparticles can be detected with high sensitivity directly in unprocessed clinical samples (e.g. blood, sputum) due to the inherently negligible magnetic susceptibility of biological material. As a result, magnetic microchip-based diagnostics have been applied with great success to the isolation and detection of rare cells and the measurement of sparse soluble proteins. In this paper, we review recent advances in microchip-based detection of magnetically labeled biomarkers and their translation to clinical applications in cancer.

Magnetic Nickel iron Electroformed Trap (MagNET): a master/replica fabrication strategy for ultra-high throughput (>100 mL h−1) immunomagnetic sorting

“…While many ingenious microfluidic devices have been used with great success to sort CTCs, 2,7,9 there is currently a mismatch between the throughput of microfluidic devices (ϕ ≅ 1 mL h −1 ) and the large sample volume of blood ( V ~ 20 mL) necessary for ultra-rare cell detection. This mismatch leads to run times unsuitable for practical use ( T > 10 h).…”

“…However, the approach is broadly applicable to any application that requires highly specific sorting of rare cells from large volume unprocessed samples, such as the diagnosis of infectious disease, environmental sensing, cancer biology, and stem cell research. 9,31 …”

Microchips and their Significance in Isolation of Circulating Tumor Cells and Monitoring of Cancers

“…Another solution is to identify new prognostic biomarkers that are certainly best harbingers of the early stages of cancer, a principle which can be applied to prevent the metastasis formation. CA19, CA20 and PSCA are among these biomarkers (Muluneh and Issadore, 2014). Recently the creation of nanowires is considered a great success as a strategy which is capable of high throughput CTC isolation.…”

Создание Совмещенной Технологии Спин-Вентильных Магниторезистивных Элементов И Микромагнитов

“…Разрабатываются и выпускаются различного типа пре-образователи магнитного поля и тока [1,2], головки считывания для магнитных дисков [3], гальванических развязок (ГР) [4]. Ведутся разработки запоминающих элементов и устройств на их основе [5], логических элементов, спиновых транзисторов, биосенсоров на ос-нове МР чувствительных элементов [6]. Выпускаются измерительные системы на основе МР преобразовате-лей: диагностика объектов с близлежащими магнитными полями, медицинские системы, компасы и т. п.…”