Microchip-based engineering of super-pancreatic islets supported by adipose-derived stem cells

Jun, Yesl; Kang, Ah Ran; Lee, Jae Seo; Park, Soon‐Jung; Lee, Dong Yun; Moon, Sung; Lee, Sang Hoon

doi:10.1016/j.biomaterials.2014.02.045

Cited by 70 publications

(74 citation statements)

References 53 publications

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“…The shape of the microwell can be modified into different shapes, including rectangles and triangles (3). Various cell types have been applied to microwells, including embryonic stem cells, islet cells and hepatocytes (5,17,18). A previous study suggested that size may be an important parameter that influences cell differentiation (19).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Single primary islet cells have been co-cultured with adipose-derived stem cells in concave microwells, resulting in improved viability and function (17,18,28). Stem cells may have significant potential to protect primary cells from damage during culturing and may be employed to improve cell survival and function prior to transplantation (17). Another study used primary pancreatic islets and hepatocytes for the three-dimensional co-culture model, and these two types of cells appeared to strongly support the functions of each other as spheroids, even though smaller proportions of each cell type were evaluated when compared to the mono-culture models (18).…”

Section: Discussionmentioning

confidence: 99%

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Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests

Lee

Yeo²,

Kim

et al. 2015

Biomedical Reports

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Abstract. Human mesenchymal stem cells have previously been isolated and characterized from the gingiva, and gingiva-derived stem cells have been applied for tissue engineering purposes. The present study was performed to generate size-controllable stem cell spheroids using concave microwells. Gingiva-derived stem cells were isolated, and the stem cells of 1x10 5 (group A) or 2x10 5 (group B) cells were seeded in polydimethylsiloxane-based, concave micromolds with 600 µm diameters. The morphology of the microspheres was viewed under an inverted microscope, and the changes in the diameter and cell viability were analyzed. The gingiva-derived stem cells formed spheroids in the concave microwells. The diameters of the spheroids were larger in group A compared to group B. No significant changes in shape or diameter were noted with increases in incubation time. Cell viability was higher in group B at each time point when compared with group A. Within the limits of the study, the size-controllable stem cell spheroids could be generated from gingival cells using microwells. The shape of the spheroids and their viability were clearly maintained during the experimental periods.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests

Lee

Yeo²,

Kim

et al. 2015

Biomedical Reports

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“…The main obstacles affected success of the strategy in T1DM is autoimmune-mediated destruction of the transplanted β-cells and pancreatic islets [54] . One of the possible causes leaded to low efficacy of stem cell transplantation is cellular damage during the isolation process and donor shortages [55] . All these stimulate efforts for creating of novel techniques for increase transplantation efficacy by co-culturing single primary islet cells with adipose-derived stem cells (ADSCs).…”

Section: Limitation Of the Cell Therapy In Dmmentioning

confidence: 99%

“…Indeed, in vivo experiments with involving xenotransplantation of microfiber-encapsulated spheroids into a mouse model of DM have found that co-culture-transplanted mice lead to higher glucose metabolism modulation when compared with mono-culture-transplanted mice. The novel method for culturing islet spheroids were tested by Jun et al [55] . Investigators concluded that new technique is potentially over helmed the traditional technologies in turn of cell shortages.…”

Section: Limitation Of the Cell Therapy In Dmmentioning

confidence: 99%

Diabetes mellitus and cellular replacement therapy: Expected clinical potential and perspectives

Berezin¹

2014

WJD

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Diabetes mellitus (DM) is the most prevailing disease with progressive incidence worldwide. Despite contemporary treatment type one DM and type two DM are frequently associated with long-term major microvascular and macrovascular complications. Currently restoration of failing β-cell function, regulation of metabolic processes with stem cell transplantation is discussed as complements to contemporary DM therapy regimens. The present review is considered paradigm of the regenerative care and the possibly effects of cell therapy in DM. Reprogramming stem cells, bone marrowderived mononuclear cells; lineage-specified progenitor cells are considered for regenerative strategy in DM. Finally, perspective component of stem cell replacement in DM is discussed.

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Pathophysiological Reconstruction of a Tissue‐Specific Multiple‐Organ On‐A‐Chip for Type 2 Diabetes Emulation using 3D Cell Printing

Kim

Park

Nguyen

et al. 2023

Adv Funct Materials

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Type 2 diabetes mellitus (T2D), a chronic complex disease with high prevalence, causes severe damage to various organs. T2D has many etiologies that have not been fully elucidated. Although various models have been created to understand T2D, reconstructing the tissue-specific microenvironment and complications of T2D remain difficult. Therefore, a dynamic multiple-organ on-a-chip that mimics T2D in a hyperglycemic environment and visceral adipose tissue (vAT) using 3D cell printing with decellularized extracellular matrix (dECM) bioinks is developed. This chip composed of separate compartments for the pancreas, adipose tissue, and liver, closely related to T2D, and a retinal compartment is added to confirm T2D complications. Furthermore, the pathological features of T2D are clearly revealed in the chip with vAT-derived dECM bioink in a hyperglycemic environment. These pathological features caused cellular dysfunctions of the retinal compartment. Moreover, treated T2D medications on the chip showed clinically identical efficacy. The chip is suitable for recapitulating the key features of T2D and is a promising platform for drug testing and T2D complication research.

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Microchip-based engineering of super-pancreatic islets supported by adipose-derived stem cells

Cited by 70 publications

References 53 publications

Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests

Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests

Diabetes mellitus and cellular replacement therapy: Expected clinical potential and perspectives

Pathophysiological Reconstruction of a Tissue‐Specific Multiple‐Organ On‐A‐Chip for Type 2 Diabetes Emulation using 3D Cell Printing

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