Microchip capillary electrophoresis (Lab-on-chip®) improves detection of celery (Apium graveolens L.) and sesame (Sesamum indicum L.) in foods

Coı̈sson, Jean Daniel; Cereti, Elisabetta; Garino, Cristiano; D’Andrea, Matteo; Recupero, M; Restani, Patrizia; Arlorio, Marco

doi:10.1016/j.foodres.2010.03.008

Cited by 16 publications

(6 citation statements)

References 32 publications

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“…A method for the simultaneous detection of celery and sesame in foods by means of an end-point PCR protocol in connection with a microchip CE platform (Coïsson et al, 2010) had a LOD of 1 mg/kg DNA for sesame.…”

Section: Multiplex Dna-based Methodsmentioning

confidence: 99%

Scientific Opinion on the evaluation of allergenic foods and food ingredients for labelling purposes

Products¹

2014

EFS2

117

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Following a request from the Food Safety Authority of Ireland, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA Panel) was asked to deliver a scientific opinion on the evaluation of allergenic foods and food ingredients for labelling purposes. In view of the request, the NDA Panel decided to update its previous opinions relative to food ingredients or substances with known allergenic potential listed in Annex IIIa of 2003/89/EC, as amended. These include cereals containing gluten, milk and dairy products, eggs, nuts, peanuts, soy, fish, crustaceans, molluscs, celery, lupin, sesame, mustard and sulphites. The opinion relates to immunoglobulin (Ig)E-and non-IgE-mediated food allergy, to coeliac disease and to adverse reactions to sulphites in food, and it does not address non-immune-mediated adverse reactions to food. It includes information on the prevalence of food allergy in unselected populations, proteins identified as food allergens, cross-reactivities, the effects of food processing on the allergenicity of foods and ingredients, methods for the detection of allergens and allergenic foods, doses observed to trigger adverse reactions in sensitive individuals and risk assessment methodologies that have been used to derive individual and population thresholds for selected allergenic foods. The present opinion relates to immunoglobulin (Ig)E-and non-IgE-mediated food allergy, to coeliac disease and to adverse reactions to sulphites in food, and it does not address non-immune-mediated adverse reactions to food. For each food ingredient or substance listed in Annex IIIa, it includes information on the prevalence of food allergy in unselected populations, on proteins identified as food allergens, on cross-reactivities, on the effects of food processing on the allergenicity of foods and ingredients, on methods for the detection of allergens and allergenic foods, and on doses observed to trigger adverse reactions in sensitive individuals.Immune-mediated adverse reactions to foods manifest with clinical signs and symptoms of variable severity and duration, which may affect different organs and systems. Anaphylactic reactions to food are IgE mediated and may occur at any age. Non-IgE-mediated food allergy includes a wide range of diseases, including protein-induced enterocolitis and eosinophilic oesophagitis.A careful family and clinical history are the basis for diagnosis of food allergy. Food diaries, skin prick tests (SPTs), allergen-specific IgE measurements, food elimination diets and food challenges are part of the standard protocol for the diagnosis of food allergy. A positive SPT indicates sensitisation to the tested food, but it is not diagnostic of food allergy. Allergen-specific serum IgE antibodies similarly denote sensitisation to a particular food, but they are not diagnostic without a clinical history or food challenge. The use of atopy patch tests for the diagnosis of food allergy is controversial. Other available tests have no current role in the diagnosis of food allergy. Diag...

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Section: Multiplex Dna-based Methodsmentioning

confidence: 99%

Scientific Opinion on the evaluation of allergenic foods and food ingredients for labelling purposes

Products¹

2014

EFS2

117

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“…The methodology involves the generation and detection of characteristic restriction patterns that enable the rapid identification of meat species. Using the same commercial CE instrument, Coïsson et al have recently analyzed DNA extracts amplified by multiplex PCR, previously isolated from cooked meat balls and soups, in order to detect traces of celery and sesame DNA 157. Similarly, Delibato et al have developed a PCR‐lab‐on‐a‐chip method to detect the food‐borne microorganism Listeria monocytogenes in food samples 158.…”

Section: Ce Microchip Technology In Food Analysismentioning

confidence: 99%

“…This paper demonstrates the applicability of chip electrophoresis with native fluorescence detection for rapid analysis of natural compounds in fruits and reveals the potential of chip‐based separation systems for the analysis of complex food mixtures. Other recent applications of chip electrophoresis in food analysis include the analysis of phenolic compounds in beer 174, the detection of celery and sesame in different foods including meat balls and commercial soup via DNA analysis 157, the direct detection of melamine in milk products 175 or the detection of nitrite in ham and sausage samples 176. These applications are good examples of the huge possibilities of chip electrophoresis in monitoring the quality of different foods.…”

Section: Foodomics and Other Future Trends Of Ce In Food Analysismentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2011

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The analysis of food components using capillary electromigration methods is reviewed in this work. Papers that were published from February 2009 to February 2011 are included following the previous review by Herrero et al. (Electrophoresis, 2010, 31, 205-228). The analysis of amino acids, biogenic amines, peptides, proteins, DNAs, carbohydrates, phenols, polyphenols, pigments, toxins, pesticides, vitamins, additives, small organic and inorganic ions and other compounds found in foods and beverages are reviewed, as well as those applications of CE for monitoring food interactions and food processing. The use of microchips, CE-MS and chiral-CE in food analysis is also discussed as well as other current and foreseen trends in this area of research including new developments in Foodomics.

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“…The design of specific primers based in the SesI gene allowed the detection of 1 mg kg −1 of sesame. Also Coïsson et al (2010) developed a microchip capillary electrophoresis (Lab-on-chip ® ) for the simultaneous detection of celery and sesame in foods with an LOD of 50 and 1 mg kg −1 , respectively. Moreover, Mustorp et al (2008) developed a real-time PCR for the detection of celery, mustard and sesame based on the two 2S albumins, Ses i 1 and Ses i 2, for the detection of sesame with an LOD of 50 mg kg −1 .…”

Section: Commercial Food Products Analysismentioning

confidence: 99%

Duplex real-time PCR method for the detection of sesame (Sesamum indicum) and flaxseed (Linum usitatissimum) DNA in processed food products

López-Calleja

Cruz

Martı́n

et al. 2015

Food Additives & Contaminants: Part A

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The development of a duplex real-time polymerase chain reaction (PCR) method allowing the simultaneous detection of sesame and flaxseed DNA in commercial food products is described. This duplex real-time PCR technique is based in the design of sesame- and flaxseed-specific primers based on the ITS1 region and two TaqMan fluorescent probes. The method was positive for sesame and flaxseed, and showed no cross-reactivity for all other heterologous plant and animal species tested. Sesame and flaxseed could be detected in a series of model samples with defined raw and heat-treated sesame in flaxseed, and flaxseed in sesame, respectively, with detection limits of 1.3 mg kg(-1) for sesame and 1.4 mg kg(-1) for flaxseed. The applicability of the assay for determining sesame and flaxseed in different food matrices was investigated by analysing a total of 238 commercial foodstuffs. This PCR method is useful for highly selective and sensitive detection of traces of sesame and flaxseed in commercial food products.

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Microchip capillary electrophoresis (Lab-on-chip®) improves detection of celery (Apium graveolens L.) and sesame (Sesamum indicum L.) in foods

Cited by 16 publications

References 32 publications

Scientific Opinion on the evaluation of allergenic foods and food ingredients for labelling purposes

Scientific Opinion on the evaluation of allergenic foods and food ingredients for labelling purposes

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Duplex real-time PCR method for the detection of sesame (Sesamum indicum) and flaxseed (Linum usitatissimum) DNA in processed food products

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