Microchip electrophoresis in low‐temperature co‐fired ceramics technology with contactless conductivity measurement

Fercher, Georg; Smetana, Walter; Vellekoop, Michael J.

doi:10.1002/elps.200800654

Cited by 15 publications

(26 citation statements)

References 29 publications

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“…The LODs (at 400 kHz and 10 V pp ) for K + , Na + , and Li + were, respectively, 3.1, 4.3, and 7.2 μmol·L −1 in less than 90 s under an electric field of 250V/cm. Fercher et al [138] described a novel microchip C 4 D device produced in low-temperature co-fired ceramics (LTCC). The application of LTCC multilayer technology allows a broad diversity of electrode arrangements inside and on top of the ceramics and increase the dielectric constant compared with glass or plastics microchips, resulting in better C 4 D sensitivity according to the authors.…”

Section: - Instrumentationmentioning

confidence: 99%

Recent developments in instrumentation for capillary electrophoresis and microchip‐capillary electrophoresis

2010

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Over the last years there has been an explosion in the number of developments and applications of capillary electrophoresis (CE) and microchip-CE. In part, this growth has been the direct consequence of recent developments in instrumentation associated with CE. This review, which is focused on contributions published in the last five years, is intended to complement the papers presented in this special issue dedicated to Instrumentation and to provide an overview on the general trend and some of the most remarkable developments published in the areas of high voltage power supplies, detectors, auxiliary components, and compact systems. It also includes few examples of alternative uses of and modifications to traditional CE instruments.

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Section: - Instrumentationmentioning

confidence: 99%

Recent developments in instrumentation for capillary electrophoresis and microchip‐capillary electrophoresis

2010

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“…With the rapid development of microfluidic and nanofluidic technologies in recent years, lab-on-a-chip devices have found extensive applications in the chemical, medicine, biological, and drug analysis fields (Fu et al 2004;Squires and Quake 2005;Whitesides 2006;Tsai et al 2008;Lin et al 2008;Hou et al 2009;Fercher et al 2009;Xuan et al 2010;Wu et al 2010;Li et al 2011). Compared to conventional large-scale instruments, microfluidic devices have many fundamental advantages, including (1) a higher throughput, (2) a reduced sample consumption, (3) a shorter analysis time, (4) the potential for in situ operation, (5) an improved performance and reliability, and (6) lower operating and manufacturing costs.…”

Section: Introductionmentioning

confidence: 99%

Rapid glucose concentration detection utilizing disposable integrated microfluidic chip

Hou

Wang

Chang

et al. 2011

Microfluid Nanofluid

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A novel three-dimensional (3D) disposable glucose concentration detection chip is presented. The chip comprises a four-layer polymethyl methacrylate (PMMA) structure and is fabricated using a commercial CO 2 laser and a hot-press bonding technique. In the proposed device, the glucose solution is injected into a double parallel connection micromixer (DPCM) and is mixed with DNS reagent by means of a self-rotation effect. An experimental platform has been created for multiple reaction process by integrating chip and micro-heater. The fluid streams exiting the two circular mixing chambers of the DPCM are then combined and mixed further at a T-type microchannel outlet before passing to a collection chamber. Numerical simulations are performed to analyze the vortex streamline distribution within the DPCM and to estimate the mixing performance. The numerical results show that a mixing efficiency as high as 92.5% can be obtained at low Reynolds numbers (Re = 12). It is found a good linear relation of R 2 = 0.9953 from the chip detection method comparing to the traditional method of R 2 = 0.9976 at DNS reagent and glucose solution volume ratio of 1:1. In addition, the experimental results show that the accuracy of the glucose concentration measurements obtained using the proposed microfluidic chip is comparable with that of the measurements obtained using a conventional large-scale detection method. Overall, the results presented in this study indicate that the DPCM chip provides a rapid and low-cost means of detecting the concentration of glucose solutions.

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“…Compared with their large-scale counterparts, microfluidic devices have many significant advantages, including a lower cost, a reduced sample and reagent consumption, and an improved sensitivity. As a result, they are widely used for performing chemical or biological assays in the biochemistry, biophysics, medicine, and life science fields Tsai et al 2007;Chen and Wang 2008;Lin et al 2009;Fercher et al 2009;Hong et al 2010;Tran et al 2010;Li et al 2011). Among the various microfluidic devices available, flow cytometers provide a high-throughput means of sorting and counting single cells, and are used extensively in the hematology, immunology, and microbiology fields (Lin and Lee 2008;Fu et al 2008;Tsai et al 2008;Chen and Wang 2009;Zhang et al 2009;Hou et al 2009;Hsu et al 2009;Wang et al, 2010;Xuan et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Microflow cytometer incorporating sequential micro-weir structure for three-dimensional focusing

Lee

Hou

Yang

et al. 2011

Microfluid Nanofluid

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A novel microflow cytometer is proposed in which the sample stream is focused initially in the horizontal (X-Y) plane by two sheath flows and is then focused in the vertical (Y-Z) plane by a sequential micro-weir structure before entering the detection region of the device. The proposed device is fabricated using a modified glass fabrication process, and is then characterized both numerically and experimentally using fluorescent polystyrene beads with diameters of 7 and 15 lm, respectively. The experimental and numerical results confirm the effectiveness of the hydrodynamic sheath flows in centralizing the particles in the horizontal plane prior to entering the sequential micro-weir structure. Furthermore, it is shown numerically that the micro-weir structure confines the particle stream to the center of the vertical plane such that the particles pass through the detection region in a oneby-one fashion can be counted with a high degree of reliability. The experimental results confirm that the proposed 3-D focusing scheme enables the fluorescent beads with different diameters to be reliably sorted and counted.

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Microchip electrophoresis in low‐temperature co‐fired ceramics technology with contactless conductivity measurement

Cited by 15 publications

References 29 publications

Recent developments in instrumentation for capillary electrophoresis and microchip‐capillary electrophoresis

Recent developments in instrumentation for capillary electrophoresis and microchip‐capillary electrophoresis

Rapid glucose concentration detection utilizing disposable integrated microfluidic chip

Microflow cytometer incorporating sequential micro-weir structure for three-dimensional focusing

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