Microcirculation of the Rat Small Intestine as Studied by the Injection Replica Scanning Electron Microscope Method

Ohashi, Yozo; Kita, Shoichi; Murakami, Takuro

doi:10.1679/aohc1950.39.271

Cited by 35 publications

(10 citation statements)

References 9 publications

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“…Thus, an extensive capillary network with fenestration is considered to function to supply the fluids required for exocrine function in the exocrine glands (Suddick and Dowd, ). The blood capillary network is also well developed in the lamina propria of the intestinal crypt base in the rat small intestine (Ohashi et al, ). In this study, blood capillaries whose endothelial cells exhibited fenestration on their surfaces adjacent to Paneth cells were frequently and closely localized beneath Paneth cells.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural and Immunohistochemical Study on the Lamina Propria Cells Beneath Paneth Cells in the Rat Ileum

Mantani

Nishida

Yamamoto

et al. 2018

The Anatomical Record

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Paneth cells secrete bactericidal substances in response to bacterial proliferation on the mucosal surface without directly contacting bacteria. However, the induction mechanism of this transient secretion has not been clarified, although nervous system and/or immunocompetent cells in the lamina propria (LP) might be involved. In this study, we ultrastructurally and immunohistochemically investigated which LP cells are localized beneath Paneth cells and examined the relationship between the Paneth cell-derived cellular processes which extended into the LP and the LP cells. The results showed that various cells-including blood capillary, subepithelial stromal cell, and nerve fiber-were present in the LP beneath Paneth cells. Endothelial cells of blood capillary were the cells most frequently found in this location; they were situated within 1 μm of the Paneth cells and possessed fenestration on the surfaces adjacent to Paneth cells. The Paneth cells rarely extended the cellular processes toward the LP across the basal lamina. Most of the cellular processes of Paneth cells contacted the subepithelial stromal cells. Immunohistochemistry revealed that the CD34 CD31 αSMA stromal cells preferentially localized in the LP beneath the intestinal crypt base, while PDGFRα αSMA stromal cells mainly localized around the lateral portions of the intestinal crypt and PDGFRα αSMA stromal cells localized in the intestinal villus. From these findings, the existence of blood capillaries beneath Paneth cells might reflect the active exocrine function of Paneth cells. Furthermore, subepithelial stromal cells, probably with a CD34 CD31 αSMA PDGFRα phenotype, beneath the crypt base might affect Paneth cell activity by interacting with their cellular processes. Anat Rec, 301:1074-1085, 2018. © 2018 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Ultrastructural and Immunohistochemical Study on the Lamina Propria Cells Beneath Paneth Cells in the Rat Ileum

Mantani

Nishida

Yamamoto

et al. 2018

The Anatomical Record

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“…The microvascular bed of the intestinal mucosa is independent from those of the tunica muscularis; both are separately supplied by and drained into the submucosal (OHASHI, KITA and MURAKAMI, 1976). The tunica muscularis has two capillary networks, an outer and inner layer of capillaries arranged along the longitudinal and circular muscles, respectively (Fig.…”

Section: Small Intestinementioning

confidence: 99%

“…It is postulated that hormones released from the basal-granulated, or endocrine cells (which are condensed at the deeper portion of the crypt and are richly supplied by a capillary network) can be efficiently transported to act in the villous base (OHASHI et al, 1976). In the rat Peyer's patch (or intestinal lymphoid follicle), one or more arterioles ascending through the follicle connect with the capillary network immediately beneath the epithelium.…”

Section: Does a Vascular Countercurrent Mechanism Operate In The Villi?mentioning

confidence: 99%

Microvasculature as studied by the microvascular corrosion casting/scanning electron microscope method. I. Endocrine and digestive system.

Ohtani

Kikuta

Ohtsuka

et al. 1983

Arch. Histol. Jap., Arch. Histol. Jpn

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“…Once the concept was born, we searched the literature and rapidly noticed that the tiny "holes" characteristic of intussusception in vascular casts could easily be made out in numerous publications about the vasculature of various organs and different animal species (see, e.g., Ohashi et al, 1976;Olson, 1980;Ohtani et al, 1983;Hossler and Olson, 1984;Takemori et al, 1984).…”

mentioning

confidence: 99%

Intussusceptive angiogenesis: Its emergence, its characteristics, and its significance

Burri

Hlushchuk

Djonov

2004

Developmental Dynamics

356

263

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This review shall familiarize the reader with the various aspects of intussusceptive angiogenesis (IA). The basic event in IA is the formation of transvascular tissue pillars. Depending on location, timing, and frequency of pillar emergence, the IA process has different outcomes. In capillaries, a primary IA function is to expand the capillary bed in size and complexity (intussusceptive microvascular growth). It represents an alternative to capillary sprouting. Highly ordered pillar formation in a developing capillary network leads to the formation of vascular trees (intussusceptive arborization). In small arteries and veins, pillar formation at the vessels' branching angles leads either to remodeling of the branching geometry or even to vascular pruning (intussusceptive branching remodeling). It appears essential that future angiogenic research considers always both phenomena, sprouting and intussusception. Vascularization of tissues, organs, and tumors rely heavily on both mechanisms; neglecting one or the other would obscure our understanding of the angiogenesis process. Developmental Dynamics 231:474 -488, 2004.

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Microcirculation of the Rat Small Intestine as Studied by the Injection Replica Scanning Electron Microscope Method

Abstract: Summary.Mucosal blood supply in the rat small intestine was studied by the injection replica scanning electron microscope method.The blood vascular plexus in the villus is originated at the tip of the villus and con-

Cited by 35 publications

References 9 publications

Ultrastructural and Immunohistochemical Study on the Lamina Propria Cells Beneath Paneth Cells in the Rat Ileum

Ultrastructural and Immunohistochemical Study on the Lamina Propria Cells Beneath Paneth Cells in the Rat Ileum

Microvasculature as studied by the microvascular corrosion casting/scanning electron microscope method. I. Endocrine and digestive system.

Intussusceptive angiogenesis: Its emergence, its characteristics, and its significance

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