Microclonal Propagation of Karelian Birch and Triploid Aspen in Vitro

Anokhina, N.S.; Konovalov, V. F.; Khanova, Elvira

doi:10.31163/2618-964x-2021-4-2-101-106

Cited by 1 publication

(1 citation statement)

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“…For the purposes of plantation cultivation, it is advisable to use modern methods of biotechnology, such as clonal micropropagation, which allows to produce a large amount of high-quality, healthy planting material, including species poorly propagated by the traditional methods, in a short time throughout a year [21]. So far, studies on the introduction of triploid forms of aspen to in vitro culture have been carried out by a number of Russian scientists from different regions of the country [1,8,10,14,17,28]. Since 2010, studies have been carried out at the Central European Forest Experimental Station of the All-Russian Research Institute for Silviculture and Mechanization of Forestry (ARRISFM) [3,16,27].…”

Section: Introductionmentioning

confidence: 99%

Features of Triploid Aspen Clonal Micropropagation Using Modern Growth-Stimulating Preparations

Макаров¹,

Bagaev²,

Chudetsky³

et al. 2023

Lesnoy Zhurnal (Forestry Journal)

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The creation of fast-growing triploid aspen plantations in the area of wood processing enterprises has high importance in terms of increasing demand for deciduous wood associated with the development of board production and the prospects for the introduction of innovative technologies for deep processing of wood. The article presents the results of studies of triploid aspen clones of Kostroma origin at the stages of “introduction to in vitro culture”, “proper micropropagation”, and “rooting of microshoots” with the use of growth-regulating substances. At the “introduction culture to in vitro” stage, the most effective sterilizing agents were silver nitrate with the concentration of 0.2 %, Lysoformin 3000, 5 % applied for 15 min and sulema, 0.2 % used for 10 min. At the “proper micropropagation” stage, the total length of triploid aspen shoots did not differ significantly depending on the composition of the nutrient media studied but was slightly exceeded the options with Woody Plant Medium. An increase by 1.2–2.6 times was observed in the total length of triploid aspen microshoots in vitro, when the concentration of 6-benzylaminopurine in the nutrient medium was increased from 0.5 to 1.0 mg/L. The maximum value of the total length (5.6 cm) of tryploid aspen microshoots in vitro was observed on Woody Plant Medium at the 6-benzylaminopurine concentration of 1.0 mg/L and the presence of Epin-Extra preparation of 0.5 mg/L. At the “rooting of microshoots” stage the number, average, and total length of triploid aspen roots in vitro had no statistically significant differences depending on the nutrient medium composition. Increasing the concentrations of indole-3-butyric acid and indole-3-acetic acid from 1.0 to 2.0 mg/L in the nutrient medium increased the total length of regenerated plant roots of triploid aspen in vitro by 2.3–2.4 times. The maximum total length (5.1 cm) of triploid aspen roots in vitro was observed on Woody Plant Medium with indole-3-butyric acid at a concentration of 1.0 mg/L. Clonal micropropagation appears to be a perspective for accelerated production of elite planting material of triploid aspen for the purposes of laying out timber plantations. For citation: Makarov S.S., Bagaev E.S., Chudetsky A.I., Kuznetsova I.B., Lebedeva O.P., Antonov A.M. Features of Triploid Aspen Clonal Micropropagation Using Modern Growth-Stimulating Preparations. Lesnoy Zhurnal = Russian Forestry Journal, 2023, no. 2, pp. 183–194. (In Russ.). https://doi.org/10.37482/0536-1036-2023-2-183-194

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