Microcontact Printing for Creation of Patterned Lipid Bilayers on Tetraethylene Glycol Self-Assembled Monolayers

Strulson, Matthew K.; Maurer, Joshua A.

doi:10.1021/la201839w

Cited by 12 publications

(20 citation statements)

References 40 publications

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“…This result means that the lower layer is as strong as that of the upper layer to be penetrated even if it is not expected since there is hydrophobic surface upside for lower layer DSPC LB film, while there is hydrophilic surface upside for upper layer. The possible reasons for that might be the water [ 28 ] and the hydration layer (formed between the mica surface and the hydrophilic surface of lipid films) [ 19 ] can affect the penetration forces.…”

Section: Resultsmentioning

confidence: 99%

“…The AFM tip is approximately considered as spherical at the very end, and then DMT (Derjaguin-Müller-Toporov) model [ 35 ] is thought as a suitable model [ 34 ] to analysis AFM tip and lipid film at the moderate adhesion levels. The DMT modulus is used to measure the Young’s modulus for materials [ 19 ]. It has been reported that the DMT modulus values obtained with MultiMode® 8 AFM at different working modes are in very good agreement with each other (HarmoniX DMT modulus mapping mode and Peak Force Tapping DMT modulus mapping mode) [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

“…The indentation force curves of DSPC monolayer, upper layer and lower layer LB films were obtained for the first time. The analysis shows that the obtained penetration distances of the indentation force curves are equal to the thickness values of DSPC LB films, while the DMT modulus [ 19 ] of DSPC upper layer is different with that of monolayer films.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nanomechanics of phospholipid LB film studied layer by layer with AFM

Zhu

et al. 2014

Chemistry Central Journal

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BackgroundPhospholipid, a main component of cell membrane, has been explored as a model system of the cell membrane and temporary scaffold materials in recent studies. The mechanical properties of phospholipid layers are essentially interesting since it is involved in several biological processes.ResultsHere, the nanomechanical properties such as indentation force, adhesion force and DMT (Derjaguin-Müller-Toporov) modulus of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) Langmuir-Blodgett (LB) films were analyzed layer by layer with Atomic Force Microscope (AFM) under deionized water condition.ConclusionsThe penetration distances in the indentation force curves are equal to the thicknesses of phospholipid films, and the yield forces of DSPC LB films in deionized water are smaller than that of similar lipid films in buffered solutions due to the influence of ions. Moreover, the DMT modulus of upper layer DSPC LB film is different from that of monolayer DSPC LB film due to the influence of their different substrates. Our results suggest that environment such as surrounding ions and substrate will strongly influence the measured nano-mechanical properties of the lipid bilayer, especially that of the down layer.Graphical AbstractA process about the exploration of nanomechanics of DSPC LB film.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nanomechanics of phospholipid LB film studied layer by layer with AFM

Zhu

et al. 2014

Chemistry Central Journal

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“…Different substrates might be used for SLB formation (i.e. glass, mica, silicon dioxide, polymers) depending on the nature of the study 4,[6][7][8] . Typical membrane studies rely on microscopy-based imaging techniques, such as TIRFM and AFM.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging

Matysik

Kraut

2014

JoVE

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Supported lipid bilayers (SLBs) are widely used as a model for studying membrane properties (phase separation, clustering, dynamics) and its interaction with other compounds, such as drugs or peptides. However SLB characteristics differ depending on the support used.Commonly used techniques for SLB imaging and measurements are single molecule fluorescence microscopy, FCS and atomic force microscopy (AFM). Because most optical imaging studies are carried out on a glass support, while AFM requires an extremely flat surface (generally mica), results from these techniques cannot be compared directly, since the charge and smoothness properties of these materials strongly influence diffusion. Unfortunately, the high level of manual dexterity required for the cutting and gluing thin slices of mica to the glass slide presents a hurdle to routine use of mica for SLB preparation. Although this would be the method of choice, such prepared mica surfaces often end up being uneven (wavy) and difficult to image, especially with small working distance, high numerical aperture lenses. Here we present a simple and reproducible method for preparing thin, flat mica surfaces for lipid vesicle deposition and SLB preparation. Additionally, our custom made chamber requires only very small volumes of vesicles for SLB formation. The overall procedure results in the efficient, simple and inexpensive production of high quality lipid bilayer surfaces that are directly comparable to those used in AFM studies. Video LinkThe video component of this article can be found at

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“…[1][2][3] Recently, PDMS with micropatterned surface topographies were exploited for applications in various unconventional lithographic methods, including soft lithography [4][5][6][7][8][9][10][11] [e.g., microcontact printing (mCP), [12][13][14] micromolding in capillaries (MIMIC) [15][16][17] and microtransfer molding (mTM) [18] ], lithographically controlled wetting [19,20] and lithographically controlled demixing, [21] which are extensively applied in bottom-up micro-and nanofabrication to generate patterns on surfaces of substrates. To date, PDMS stamps have been used to spatially pattern a broad range of materials, including thiols, [22] silanes, [23] nanoparticles, [24] metal thin films, [25] polymers, [26] proteins, [27] DNA [28] and bacteria, [29] onto material surfaces using mCP and MIMIC techniques. Some important classes of functional materials are currently processed by PDMS stamps.…”

Section: Introductionmentioning

confidence: 99%

A Simple Method for Fabricating Patterned Curvilinear Microstructures in Poly(dimethylsiloxane) by Selective Wetting

Tang

2013

ChemPhysChem

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The fabrication of patterned microstructures in poly(dimethylsiloxane) (PDMS) is a prerequisite for soft lithography. Herein, curvilinear surface relief microstructures in PDMS are fabricated through a simple three-stage approach combining microcontact printing (μCP), selective surface wetting/dewetting and replica molding (REM). First, using an original PDMS stamp (first-generation stamp) with linear relief features, a chemical pattern on gold substrate is generated by μCP using hexadecanethiol (HDT) as an ink. Then, by a dip-coating process, an ordered polyethylene glycol (PEG) polymer-dot array forms on the HDT-patterned gold substrate. Finally, based on a REM process, the PEG-dot array on gold substrate is used to fabricate a second-generation PDMS stamp with microcavity array, and the second-generation PDMS stamp is used to generate third-generation PDMS stamp with microbump array. These fabricated new-generation stamps are utilized in μCP and in micromolding in capillaries (MIMIC), allowing the generation of surface micropatterns which cannot be obtained using the original PDMS stamp. The method will be useful in producing new-generation PDMS stamps, especially for those who want to use soft lithography in their studies but have no access to the microfabrication facilities.

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Microcontact Printing for Creation of Patterned Lipid Bilayers on Tetraethylene Glycol Self-Assembled Monolayers

Cited by 12 publications

References 40 publications

Nanomechanics of phospholipid LB film studied layer by layer with AFM

Nanomechanics of phospholipid LB film studied layer by layer with AFM

Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging

A Simple Method for Fabricating Patterned Curvilinear Microstructures in Poly(dimethylsiloxane) by Selective Wetting

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