Nanomechanics of phospholipid LB film studied layer by layer with AFM

Li, Yinli; Zhu, Changjiang; Zhu, Jiang; Liang, Hsin-Yi; Chen, Dong; Zhao, Huiling; Liu, Bo

doi:10.1186/s13065-014-0071-2

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…The adhesive response for erucamide monolayer was likely affected by the underlying silica surface, thus slightly higher than that obtained for the bilayers or multilayers, for which the adhesive properties became dominated by the erucamide layers. This is consistent with report by Balzer et al in their AFM adhesion study on different layers of thin polymer films supported by silica [60], as well as the study by Li et al on the adhesion of Langmuir-Blodgett phospholipid layers atop mica [61]. The adhesion of erucamide multilayers appeared to depend on the local curvature, with a higher value obtained on the bilayer edge than on its top, resulting in pronounced adhesive response peak (marked as an asterisk) in the adhesive response profile (Figure 8D), possibly as a consequence of a higher adhesive response between the silicon AFM tip and the exposed erucamide hydrophilic headgroups at the edges of the multilamellar structure (Figure 8F).…”

Section: Multilayer Structure At Higher Erucamide Concentrationssupporting

confidence: 93%

Heads or tails: Nanostructure and molecular orientations in organised erucamide surface layers

Gubala

Fox

Harniman

et al. 2021

Journal of Colloid and Interface Science

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Section: Multilayer Structure At Higher Erucamide Concentrationssupporting

confidence: 93%

Heads or tails: Nanostructure and molecular orientations in organised erucamide surface layers

Gubala

Fox

Harniman

et al. 2021

Journal of Colloid and Interface Science

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“…AFM-based SMFS stands out among various single-molecule techniques [74][75][76][77] because of its high detection rate, easy operation, and wide application in measuring weak inter-or intramolecule interactions [78][79][80]. Through SMFS technique, multiple properties, such as elasticity and viscosity [81], can be analyzed in detail. At the same time, SMFS is analytic technique applied not only to measure mechanical properties of various proteins but also to manipulate single-molecule at pico-Newton scale [78], for example, probing the helical structure, unfolding -fold structure [64], and measuring intermolecular interactions.…”

Section: Principle Of Afm-based Smfsmentioning

confidence: 99%

Characterization of Inter- and Intramolecular Interactions of Amyloid Fibrils by AFM-Based Single-Molecule Force Spectroscopy

Liang

Zhao

et al. 2016

Journal of Nanomaterials

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Amyloids are fibrous protein aggregates defined by shared specific structural features. Abnormal accumulation of amyloid in organs leads to amyloidosis, which results in various neurodegenerative diseases. Atomic force microscopy (AFM) has proven to be an excellent tool investigating amyloids; it has been extensively utilized to characterize its morphology, assembly process, and mechanical properties. This review summarizes studies which applied AFM to detect the inter- and intramolecular interactions of amyloid fibrils and classified the influencing factors of amyloid’s nanomechanics in detail. The characteristics of amyloid fibrils driven by inter- and intramolecular interactions, including various morphologies of amyloid fibrils, self-assembly process, and the aggregating pathway, are described. Successful examples where AFM provided abundant information about inter- and intramolecular interactions of amyloid fibrils in different environments are presented. Direct force measurement of intra- or intermolecular interactions utilizing an AFM-based tool, single-molecular force spectroscopy (SMFS), is introduced. Some mechanical information such as elasticity, adhesiveness, and strength was obtained by stretching amyloid fibrils. This review helps researchers in understanding the mechanism of amyloidogenesis and exploring the properties of amyloid using AFM techniques.

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“…Thus, SPBs are prone to be affected by their underlying substrates; it has been shown that physical properties of SPBs such as gel-to-fluid transition temperature and lateral diffusion of phospholipid molecules are influenced by their supporting substrates. The significant effects of substrate on the mechanical properties of SPBs have also been reported. − For example, the extent of deformation in an SPB (which could affect its integrity) upon interaction with an external object (such as NPs or macromolecules) is determined by its stiffness, which can be influenced by the degree of interaction of the bilayer with its underlying substrate. Although there are few reports on the effect of substrate properties such as chemical composition, hydrophobicity, and roughness on spontaneous formation of SPBs, the effect of substrate interactions of SPBs and their influence on membrane stability have been mostly neglected in studies where SPBs were used as model cell membranes .…”

Section: Introductionmentioning

confidence: 99%

Toward More Free-Floating Model Cell Membranes: Method Development and Application to Their Interaction with Nanoparticles

Yousefi

Wargenau

Tufenkji

2016

ACS Appl. Mater. Interfaces

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Identifying the mechanisms of nanoparticle (NP) interactions with cell membranes is key to understanding potential NP cytotoxicity and applications as nanocarriers for targeted drug delivery. To elucidate these mechanisms of interaction, supported phospholipid bilayers (SPBs) are commonly used as models of cell membranes. However, SPBs are soft thin films, and, as such, their properties can be significantly affected by the underlying substrate. Free-floating cell membranes would be best modeled by weakly adhered SPBs; thus, we propose a method for tailoring the interfacial interaction of an electrically charged SPB-substrate system based on modulations in the solution chemistry. Using the dissipation signal of the quartz crystal microbalance with dissipation monitoring (QCM-D), we show that the method can be used to tailor SPB-substrate interactions without the loss of its structural integrity. To demonstrate the application of the method, SPBs are exposed to cationic and anionic polystyrene latex NPs. These studies reveal that the bilayer response to the modulations in the interfacial interaction with its underlying substrate can be used as a sensitive tool to probe the integrity of SPBs upon exposure to NPs. As expected, anionic NPs tend to impart no significant damage to the anionic bilayers, whereas cationic NPs can be detrimental to bilayer integrity. This is the first report of a QCM-D based method to probe bilayer integrity following exposure to NPs. Importantly, the degree of SPB interaction with its underlying substrate is shown to be a critical factor in the kinetics of bilayer disruption by cationic NPs, whereby weakly adhered bilayers are prone to significantly faster breakup. Since free-floating cell membranes are better represented by a weakly adhered SPB, the results of this work critically influence paradigms in experimental studies involving SPBs as models for cell membranes.

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Nanomechanics of phospholipid LB film studied layer by layer with AFM

Cited by 5 publications

References 38 publications

Heads or tails: Nanostructure and molecular orientations in organised erucamide surface layers

Heads or tails: Nanostructure and molecular orientations in organised erucamide surface layers

Characterization of Inter- and Intramolecular Interactions of Amyloid Fibrils by AFM-Based Single-Molecule Force Spectroscopy

Toward More Free-Floating Model Cell Membranes: Method Development and Application to Their Interaction with Nanoparticles

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