Microcontact printing of proteins on oxygen plasma-activated poly(methyl methacrylate)

Schmalenberg, Kristine E.; Buettner, Helen M.; Uhrich, Kathryn E.

doi:10.1016/j.biomaterials.2003.08.048

Cited by 109 publications

(96 citation statements)

References 34 publications

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“…Some groups have also investigated the aging behaviour of oxygen plasma treated PMMA surfaces for different periods of times. Both Schmalenberg et al 8 and Tsougeni et al 9,23 reported that the hydrophobic recovery begun almost immediately after relatively short exposure (30 s-5 min). On the other hand, longer treatments (20 min-60 min) were reported to make the surface superhydrophilic for several days.…”

Section: B Pmmamentioning

confidence: 99%

“…[6][7][8][9]21,23,36 Several studies 9,21,23 have demonstrated that longer treatment times induce more hydrophilic surfaces. Similar results have been observed for higher plasma powers as compared with lower ones, 6 implicating that the higher the plasma dose the lower the measured contact angle.…”

Section: B Pmmamentioning

confidence: 99%

“…In biomedical applications, plasma hydrophilization can improve the biocompatibility of polymers [1][2][3] as well as affect the attachment density of cells [4][5][6][7] and the adsorption of proteins. 8,9 Capillary filling of polymer microfluidic channels [10][11][12][13] and structured substrates 9,14,15 is also enhanced by hydrophilization, since most polymers in their native form are either hydrophobic or only slightly hydrophilic. Furthermore, plasma hydrophilization can be used for improved adhesion and bonding of polymers.…”

Section: Introductionmentioning

confidence: 99%

“…The polymers chosen for the study were: (1) polydimethylsiloxane (PDMS), (2) polymethylmethacrylate (PMMA), (3) polycarbonate (PC), (4) polyethylene (PE), (5) polypropylene (PP), (6) polystyrene (PS), (7) epoxy polymer SU-8, (8) hybrid polymer ORMOCOMP, (9) polycaprolactone (PCL), and (10) a branched poly(e-caprolactone/D,L-lactide)copolymer with 70/30 monomer ratio (P(CL/DLLA)).…”

Section: Introductionmentioning

confidence: 99%

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Oxygen and nitrogen plasma hydrophilization and hydrophobic recovery of polymers

Jokinen

Suvanto²,

Franssila³

2012

Biomicrofluidics

168

118

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Plasma hydrophilization and subsequent hydrophobic recovery are studied for ten different polymers of microfabrication interest: polydimethylsiloxane (PDMS), polymethylmethacrylate, polycarbonate, polyethylene, polypropylene, polystyrene, epoxy polymer SU-8, hybrid polymer ORMOCOMP, polycaprolactone, and polycaprolactone/D,L-lactide (P(CL/DLLA)). All polymers are treated identically with oxygen and nitrogen plasmas, in order to make comparisons between polymers as easy as possible. The primary measured parameter is the contact angle, which was measured on all polymers for more than 100 days in order to determine the kinetics of the hydrophobic recovery for both dry stored and rewashed samples. Clear differences and trends are observed both between different polymers and between different plasma parameters.

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Section: B Pmmamentioning

confidence: 99%

Section: B Pmmamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Oxygen and nitrogen plasma hydrophilization and hydrophobic recovery of polymers

Jokinen

Suvanto²,

Franssila³

2012

Biomicrofluidics

168

118

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“…Significantly, it has been shown elsewhere that denaturation of the α 6 β 1 laminin binding domain, which is recognized by the AL-4 antibody, is sufficient to arrest neuronal cell attachment and neurite extension in the absence of other laminin sub-domains [18]. Curiously, others have demonstrated the successful outgrowth of neurons on μCP laminins, but only in cases where polar groups were introduced into the stamp material, onto the pattern substratum, or directly into the laminin molecule [38,39], indicating the importance of electrostatic interfacial interactions on laminin structure and resulting activity.…”

Section: Laminin Activity Dependence On Epitope Presentationmentioning

confidence: 99%

The differential influence of colocalized and segregated dual protein signals on neurite outgrowth on surfaces

2007

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We present an in vitro micropatterning approach in which the density and spatial presentation of two separate protein layers can be independently controlled to form cell stripe assays through (1) the simultaneous application of microcontact printing (μCP) and microfluidic network patterning (μFN) to generate alternating stripes of pure single protein layers or (2) through μCP onto a pre-adsorbed homogeneous protein layer to generate alternating single and dual protein stripes. This approach enabled the creation of choice boundaries in which protein-protein interactions were limited and the effects of spatially segregated or colocalized dual protein signals on model primary neuronal behavior could be readily interrogated and compared on both glass and tissue culture polystyrene substrates. Dorsal root ganglion (DRG) cell body attachment was dictated largely by non-specific cell adhesion interactions and interactions between the guidance molecules laminin and aggrecan were insufficient to explain aggrecan inhibition on neurite outgrowth. The presentation of a specific laminin epitope stabilized by interactions with aggrecan and destabilized by μCP was a strong predictor of neurite promoting activity. These observations provide evidence that aggrecan is intrinsically inhibitory and that laminin-aggrecan interactions do not diminish laminin growth promoting properties.

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Immobilization and Patterning of Biomolecules on Surfaces

Rożkiewicz¹,

Ravoo²,

Reinhoudt³

2010

The Supramolecular Chemistry of Organic‐Inorganic Hybrid Materials

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Microcontact printing of proteins on oxygen plasma-activated poly(methyl methacrylate)

Cited by 109 publications

References 34 publications

Oxygen and nitrogen plasma hydrophilization and hydrophobic recovery of polymers

Oxygen and nitrogen plasma hydrophilization and hydrophobic recovery of polymers

The differential influence of colocalized and segregated dual protein signals on neurite outgrowth on surfaces

Immobilization and Patterning of Biomolecules on Surfaces

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