Microcontact surface imprinting of affinity peptide for electrochemical impedimetric detection of neutrophil gelatinase-associated lipocalin

Yang, Jin Chul; Cho, Chae Hwan; Choi, Doo Young; Park, Jong Pil; Park, Jin‐Young

doi:10.1016/j.snb.2022.131916

Cited by 19 publications

(13 citation statements)

References 42 publications

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“…This trend continues until a saturation point is reached, usually around 35 μM, where most of the binding sites are occupied. At higher peptide concentrations, particularly beyond 50 μM, the presence of a high density of peptide molecules can promote peptide–peptide interactions. , These interactions can cause steric hindrance or aggregation of the peptides, resulting in reduced access to binding sites on the hydrogel. In summary, to preclude the nonspecific interaction and make the peptide-hydrogel fluorescent sensor more sensitive, 10% PEG 6 kDa and 35 μM BP4 were selected for subsequent experiments.…”

Section: Resultsmentioning

confidence: 99%

Affinity Peptide-Tethered Suspension Hydrogel Sensor for Selective and Sensitive Detection of Influenza Virus

Kim,

Jeong,

Hwang

et al. 2023

ACS Appl. Mater. Interfaces

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Influenza viruses are known to cause pandemic flu outbreaks through both inter-human and animal-to-human transmissions. Therefore, the rapid and accurate detection of such pathogenic viruses is crucial for effective pandemic control. Here, we introduce a novel sensor based on affinity peptide-immobilized hydrogel microspheres for the selective detection of influenza A virus (IAV) H3N2. To enhance the binding affinity performance, we identified novel affinity peptides using phage display and further optimized their design. The functional hydrogel microspheres were constructed using the drop microfluidic technique, employing a structure composed of natural (chitosan) and synthetic (poly(ethylene glycol) diacrylate and PEG 6 kDa) polymers with the activation of azadibenzocyclooctyne for the subsequent click chemistry reaction. The binding peptide-immobilized hydrogel microsphere (BP-Hyd) was characterized by field emission scanning electron microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy and exhibited selective detection capability for the IAV H3N2. To capture the detected IAV H3N2, a Cy3-labeled IAV hemagglutinin antibody was utilized. By incorporating the affinity peptide with hydrogel microspheres, we achieved quantitative and selective detection of IAV H3N2 with a detection limit of 1.887 PFU mL–1. Furthermore, the developed suspension sensor exhibited excellent reproducibility and showed reusability potential. Our results revealed that the BP-Hyd-based fluorescence sensor platform could be feasibly employed to detect other pathogens because the virus-binding peptides can be easily replaced with other peptides through phage display, enabling selective and sensitive binding to different targets.

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Section: Resultsmentioning

confidence: 99%

Affinity Peptide-Tethered Suspension Hydrogel Sensor for Selective and Sensitive Detection of Influenza Virus

Kim,

Jeong,

Hwang

et al. 2023

ACS Appl. Mater. Interfaces

Self Cite

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“…Moreover, it can provide additional features by integrating other functions. Therefore, synthesized MIPs have been used in a wide range of applications [ 62 ], including affinity separation [ 63 – 65 ], sensing [ 66 – 68 ], and drug delivery systems [ 27 , 69 ]. Surface imprinting can be divided into two steps: (i) immobilization of the protein template on the solid substrate surface and (ii) polymerization of a thin polymer layer around the immobilized template molecules [ 70 ].…”

Section: Strategies For the Preparation Of Molecularly Imprinted Poly...mentioning

confidence: 99%

Molecularly imprinted polymers (MIPs): emerging biomaterials for cancer theragnostic applications

et al. 2023

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Cancer is a disease caused by abnormal cell growth that spreads through other parts of the body and threatens life by destroying healthy tissues. Therefore, numerous techniques have been employed not only to diagnose and monitor the progress of cancer in a precise manner but also to develop appropriate therapeutic agents with enhanced efficacy and safety profiles. In this regard, molecularly imprinted polymers (MIPs), synthetic receptors that recognize targeted molecules with high affinity and selectivity, have been intensively investigated as one of the most attractive biomaterials for theragnostic approaches. This review describes diverse synthesis strategies to provide the rationale behind these synthetic antibodies and provides a selective overview of the recent progress in the in vitro and in vivo targeting of cancer biomarkers for diagnosis and therapeutic applications. Taken together, the topics discussed in this review provide concise guidelines for the development of novel MIP-based systems to diagnose cancer more precisely and promote successful treatment. Graphical Abstract Molecularly imprinted polymers (MIPs), synthetic receptors that recognize targeted molecules with high affinity and selectivity, have been intensively investigated as one of the most attractive biomaterials for cancer theragnostic approaches. This review describes diverse synthesis strategies to provide the rationale behind these synthetic antibodies and provides a selective overview of the recent progress in the in vitro and in vivo targeting of cancer biomarkers for diagnosis and therapeutic applications. The topics discussed in this review aim to provide concise guidelines for the development of novel MIP-based systems to diagnose cancer more precisely and promote successful treatment.

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“…Label-free electrochemical detection of biomolecules either uses the change in the square wave voltammetry (SWV) reduction peak current of a redox probe or monitors the change in real and imaginary impedance (EIS) during immunorecognition events. Moreover, SWV-EIS transductions are considered more sensitive than commercially available ELISA kits [ 25 , 26 , 27 , 28 ], while amperometric sensing (current signal measurements) [ 29 , 30 , 31 , 32 ] and differential pulse voltammetry (DPV) [ 33 , 34 , 35 ] are considered advantageous as low-cost technologies and in good agreement with ELISA performance. Herein, several electrochemical studies using the Cys/GA reagents are reported and discussed ( Table 2 ).…”

Section: Electrochemical Immunosensorsmentioning

confidence: 99%

Use of Cysteamine and Glutaraldehyde Chemicals for Robust Functionalization of Substrates with Protein Biomarkers—An Overview on the Construction of Biosensors with Different Transductions

Ionescu

2022

Biosensors

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Currently, several biosensors are reported to confirm the absence/presence of an abnormal level of specific human biomarkers in research laboratories. Unfortunately, public marketing and/or pharmacy accessibility are not yet possible for many bodily fluid biomarkers. The questions are numerous, starting from the preparation of the substrates, the wet/dry form of recognizing the (bio)ligands, the exposure time, and the choice of the running buffers. In this context, for the first time, the present overview summarizes the pre-functionalization of standard and nanostructured solid/flexible supports with cysteamine (Cys) and glutaraldehyde (GA) chemicals for robust protein immobilization and detection of biomarkers in body fluids (serum, saliva, and urine) using three transductions: piezoelectrical, electrochemical, and optical, respectively. Thus, the reader can easily access and compare step-by-step conjugate protocols published over the past 10 years. In conclusion, Cys/GA chemistry seems widely used for electrochemical sensing applications with different types of recorded signals, either current, potential, or impedance. On the other hand, piezoelectric detection via quartz crystal microbalance (QCM) and optical detection by surface plasmon resonance (LSPR)/surface-enhanced Raman spectroscopy (SERS) are ultrasensitive platforms and very good candidates for the miniaturization of medical devices in the near future.

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Microcontact surface imprinting of affinity peptide for electrochemical impedimetric detection of neutrophil gelatinase-associated lipocalin

Cited by 19 publications

References 42 publications

Affinity Peptide-Tethered Suspension Hydrogel Sensor for Selective and Sensitive Detection of Influenza Virus

Affinity Peptide-Tethered Suspension Hydrogel Sensor for Selective and Sensitive Detection of Influenza Virus

Molecularly imprinted polymers (MIPs): emerging biomaterials for cancer theragnostic applications

Use of Cysteamine and Glutaraldehyde Chemicals for Robust Functionalization of Substrates with Protein Biomarkers—An Overview on the Construction of Biosensors with Different Transductions

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