MicroCT quantification of in vitro bone resorption of neonatal murine calvaria exposed to IL-1 or PTH

Stock, Stuart R.; Ignatiev, K.; Foster, Shirley A.; Forman, L.A.; Stern, Paula H.

doi:10.1016/j.jsb.2004.03.003

Cited by 29 publications

(16 citation statements)

References 39 publications

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“…mCT allows the qualitative and quantitative assessment of the spatial and temporal mineralization of bone formation. The availability of 3D analysis techniques, coupled to specific image processing methods, opens up new possibilities for the analysis of bone structures 90,91 Recent reports outline the use of mCT for the monitorization of the in vivo bone remodelling process. 92 In Figure 6, mCT-reconstructed 3D images of rat calvarial bone are shown.…”

Section: Radiological Techniquesmentioning

confidence: 99%

Rodent models in bone-related research: the relevance of calvarial defects in the assessment of bone regeneration strategies

2011

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In vivo research with animal models has been a preferred experimental system in bone-related biomedical research since, by approximation, it allows relevant data gathering regarding physiological and pathological conditions that could be of use to establish more effective clinical interventions. Animal models, and more specifically rodent models, have been extensively used and have contributed greatly to the development and establishment of a wide range of translational approaches aiming to regenerate the bone tissue. In this regard, the calvarial defect model has found great application in basic and applied research, nonetheless the controversial rationalization for the use of critical size defects -defects that are unable to report spontaneous healing -or subcritical size defects in the proposed applications. Accordingly, this work aims to review the advantages and limitations of the use of rodent models in biomedical bone-related research, emphasizing the problematic issues of the use of calvarial critical and subcritical size defects. Additionally, surgical protocols for the establishment of both defects in rat calvarial bone, as well as the description and exemplification of the most frequently used techniques to access the bone tissue repair, are portrayed.

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Section: Radiological Techniquesmentioning

confidence: 99%

Rodent models in bone-related research: the relevance of calvarial defects in the assessment of bone regeneration strategies

2011

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“…An alternative approach is to study intact bones in a developing embryo with X-ray microtomography (XMT) [5,6]. In XMT images, contrast arises from differences in the X-ray absorption of elements in the absorbing phase.…”

Section: Introductionmentioning

confidence: 99%

Manganese-enhanced magnetic resonance microscopy of mineralization

Chesnick

Todorov

Centeno

et al. 2007

Magnetic Resonance Imaging

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“…21 For laboratory sources, the resolution is typically of the order of 10 µm if applied to (biomineralized) objects with a size of millimeters to centimeters. [22][23][24][25] For synchrotron radiation sources, the resolution is of the order of 1-3 µm for millimeter-sized samples. 18,[25][26][27] A resolution of 700 nm was also recently reported.…”

Section: Basic Principles Of Microcomputer Tomography (µCt)mentioning

confidence: 99%

X-ray Microcomputer Tomography for the Study of Biomineralized Endo- and Exoskeletons of Animals

Neues

Epple

2008

Chem. Rev.

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MicroCT quantification of in vitro bone resorption of neonatal murine calvaria exposed to IL-1 or PTH

Cited by 29 publications

References 39 publications

Rodent models in bone-related research: the relevance of calvarial defects in the assessment of bone regeneration strategies

Rodent models in bone-related research: the relevance of calvarial defects in the assessment of bone regeneration strategies

Manganese-enhanced magnetic resonance microscopy of mineralization

X-ray Microcomputer Tomography for the Study of Biomineralized Endo- and Exoskeletons of Animals

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