Manganese-enhanced magnetic resonance microscopy of mineralization

Chesnick, Ingrid E.; Todorov, Todor I.; Centeno, José A.; Newbury, Dale E.; Small, John A.; Potter, Kimberlee

doi:10.1016/j.mri.2006.11.002

Cited by 3 publications

(4 citation statements)

References 45 publications

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“…The manganese content of Mn-treated DIV4 (965±56 ppm) and DIV11 (950±28 ppm) were comparable ( p =NS) and significantly higher than that of DIV18 (710±32 ppm; p <0.001) and DIV25 (688±38 ppm; p <0.001) specimens. Notably, these manganese contents were lower than what was previously reported for calvariae treated continuously with 1 mM doses of MnCl 2 [6]. …”

Section: Resultscontrasting

confidence: 71%

“…In an earlier publication, we established that manganese added to culture medium is taken up by primary osteoblasts and is deposited in the mineral phase found on the surface of organ culture calvariae [6]. The manganese taken up by the cells was detectable by MRI due to the effective relaxation sink afforded by manganese ions bound to cytoplasmic proteins.…”

Section: Discussionmentioning

confidence: 99%

“…We would like to present an alternative approach to the study of bone mineralization, namely, manganese-enhanced MRI (MEMRI). In our earlier work, we found that the addition of manganese to the culture medium can sensitize the MRI technique to newly deposited manganese-containing mineral formed during the mineralization process [4–6]. In this work, we propose to use MEMRI to map the spatial variation in mineralizing activity for calvarial skull bones maintained in organ culture.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, radioactive 54 Mn 2+ can be taken up and transported by olfactory neurons [8], providing ample evidence to support of the use of paramagnetic Mn 2+ as a surrogate for Ca 2+ for studying axonal transport in dedicated neuronal pathways [9–11], calcium-stimulated insulin secretion by pancreatic β cells [12,13], and cardiac function in stimulated myocytes [14–16]. Our group has shown that manganese can be used to sensitize the MRI technique to the mineralization process because it can be taken up by voltage-gated calcium channels on osteoblasts [6]. However, the putative toxicity of high doses (1mM) of manganese precludes its use in studying the dynamics of the mineralization process.…”

Section: Introductionmentioning

confidence: 99%

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Spatial mapping of mineralization with manganese-enhanced magnetic resonance imaging

Chesnick

Centeno

Todorov

et al. 2011

Bone

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Paramagnetic manganese can be employed as a calcium surrogate to sensitize the magnetic resonance imaging (MRI) technique to the processing of calcium during the bone formation process. At low doses, after just 48 h of exposure, osteoblasts take up sufficient quantities of manganese to cause marked reductions in the water proton T1 values compared with untreated cells. After just 24 h of exposure, 25 μM MnCl2 had no significant effect on cell viability. However, for mineralization studies 100 μM MnCl2 was used to avoid issues of manganese depletion in calvarial organ cultures and a post-treatment delay of 48 h was implemented to ensure that manganese ions taken up by osteoblasts is deposited as mineral. All specimens were identified by their days in vitro (DIV). Using inductively coupled plasma optical emission spectroscopy (ICP-OES), we confirmed that Mn-treated calvariae continued to deposit mineral in culture and that the mineral composition was similar to that of age-matched controls. Notably there was a significant decrease in the manganese content of DIV18 compared with DIV11 specimens, possibly relating to less manganese sequestration as a result of mineral maturation. More importantly, quantitative T1 maps of Mn-treated calvariae showed localized reductions in T1 values over the calvarial surface, indicative of local variations in the surface manganese content. This result was verified with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). We also found that ΔR1 values, calculated by subtracting the relaxation rate of Mn-treated specimens from the relaxation rate of age-matched controls, were proportional to the surface manganese content and thus mineralizing activity. From this analysis, we established that mineralization of DIV4 and DIV11 specimens occurred in all tissue zones, but was reduced for DIV18 specimens because of mineral maturation with less manganese sequestration. In DIV25 specimens, active mineralization was observed for the expanding superficial surface and ΔR1 values were increased due to the mineralization of small, previously unmineralized areas. Our findings support the use of manganese-enhanced MRI (MEMRI) to study well-orchestrated mineralizing events that occur during embryonic development. In conclusion, MEMRI is more sensitive to the study of mineralization than traditional imaging approaches.

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Section: Resultscontrasting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spatial mapping of mineralization with manganese-enhanced magnetic resonance imaging

Chesnick

Centeno

Todorov

et al. 2011

Bone

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Multifarious roles of metal elements in bone mineralization

Niu

Cao

et al. 2023

Applied Materials Today

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Bone-Seeking Probes for Optical and Magnetic Resonance Imaging

Kubíček

Lukeš

2010

Future Med. Chem.

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In clinical practice the imaging of bone tissue is based almost exclusively on x-ray or radiochemical methods. Alternative methods, such as MRI and optical imaging, can provide not only anatomical, but also physiological information, due to their ability to reflect the properties of body fluids (temperature, pH and concentration of ions). In this article we review bone targeting probes for MRI and fluorescence imaging. As bone targeting is mainly associated with phosphonate and bisphosphonate derivatives, we also focus on their sorption behavior. Also discussed in detail is the limitation of using bone-targeting probes for MRI and optical imaging mainly due to their long-time retention in bone tissue and the low permeability of tissues for light.

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Manganese-enhanced magnetic resonance microscopy of mineralization

Cited by 3 publications

References 45 publications

Spatial mapping of mineralization with manganese-enhanced magnetic resonance imaging

Spatial mapping of mineralization with manganese-enhanced magnetic resonance imaging

Multifarious roles of metal elements in bone mineralization

Bone-Seeking Probes for Optical and Magnetic Resonance Imaging

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