Microcystin-LR bioconcentration induces antioxidant responses in the digestive gland of two marine bivalves Crassostrea gigas and Mytilus edulis

Kim, Won Jin; Shin, Yun Kyung; Lee, Do-Hee; Kim, Youn-Jung; Kim, Jang K.; Rhee, Jae-Sung

doi:10.1016/j.aquatox.2017.05.003

Cited by 34 publications

(15 citation statements)

References 54 publications

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“…Dose-dependent increases in CAT and SOD in response to chlorothalonil could be interesting indicators of antioxidant defenses and cellular stress, as they are the first line of defense against oxygen toxicity [30, 41]. Strong enzymatic sensitivity of CAT and SOD has been reported in bivalves.…”

Section: Discussionmentioning

confidence: 99%

“…Chemicals in bivalve body and seawater have been periodically monitored by the National Institute of Fisheries Science (South Korea), as the bivalves are economically important. Adult oysters (≈ 95.4 ± 16.2 mm in length) and blue mussels (≈ 104.4 ± 14.5 mm in length) were reared in filtered seawater aerated by the automated culture system of the Southeast Sea Fisheries Research Institute of the National Institute of Fisheries Science (Tongyeong, South Korea) for 20 days before experiment [40, 41]. The acclimated conditions of the bivalves were maintained at 30 practical salinity units (psu), 20 °C, and 16:8 h light:dark photoperiod.…”

Section: Methodsmentioning

confidence: 99%

“…After chlorothalonil exposure at different concentrations, total glutathione (GSH) content and the activity of several enzymes (catalase, CAT; superoxide dismutase, SOD; glutathione peroxidase, GPx; glutathione reductase, GR) were measured according to our previous study, with slight modifications (e.g., buffer volume, employed basic instruments) [40, 41]. Gill tissue was dissected from three pooled bivalve specimens per group and was independently analyzed.…”

Section: Methodsmentioning

confidence: 99%

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Chlorothalonil induces oxidative stress and reduces enzymatic activities of Na+/K+-ATPase and acetylcholinesterase in gill tissues of marine bivalves

et al. 2019

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Chlorothalonil is a thiol-reactive antifoulant that disperses widely and has been found in the marine environment. However, there is limited information on the deleterious effects of chlorothalonil in marine mollusks. In this study, we evaluated the effects of chlorothalonil on the gill tissues of the Pacific oyster, Crassostrea gigas and the blue mussel, Mytilus edulis after exposure to different concentrations of chlorothalonil (0.1, 1, and 10 μg L −1 ) for 96 h. Following exposure to 1 and/or 10 μg L −1 of chlorothalonil, malondialdehyde (MDA) levels significantly increased in the gill tissues of C . gigas and M . edulis compared to that in the control group at 96 h. Similarly, glutathione (GSH) levels were significantly affected in both bivalves after chlorothalonil exposure. The chlorothalonil treatment caused a significant time- and concentration-dependent increase in the activity of enzymes, such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR), in the antioxidant defense system. Furthermore, 10 μg L −1 of chlorothalonil resulted in significant inhibitions in the enzymatic activity of Na + /K + -ATPase and acetylcholinesterase (AChE). These results suggest that chlorothalonil induces potential oxidative stress and changes in osmoregulation and the cholinergic system in bivalve gill tissues. This information will be a useful reference for the potential toxicity of chlorothalonil in marine bivalves.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Chlorothalonil induces oxidative stress and reduces enzymatic activities of Na+/K+-ATPase and acetylcholinesterase in gill tissues of marine bivalves

et al. 2019

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“…Recent laboratory studies have found that the toxic species associated with HABs and their secreted toxins could alter antioxidant responses and lead to cellular damage in marine organisms ( Estrada et al, 2007 ; Amado and Monserrat, 2010 ; Fabioux et al, 2015 ; Kim et al, 2017 ). For example, PST-exposed clam Ruditapes philippinarum displayed significantly altered antioxidant enzyme activity and increased LPO levels ( Choi et al, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

Integrative Biomarker Assessment of the Influence of Saxitoxin on Marine Bivalves: A Comparative Study of the Two Bivalve Species Oysters, Crassostrea gigas, and Scallops, Chlamys farreri

et al. 2018

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Harmful algae blooms have expanded greatly in recent decades, and their secreted toxins pose a severe threat to human health and marine ecosystems. Saxitoxin (STX) is a main paralytic shellfish poison naturally produced by marine microalgae of the genus Alexandrium. Despite numerous studies have assessed the impacts of STX on marine bivalves, comparative in vivo study on the toxicity of STX on bivalves with distinct accumulation ability (such as oysters and scallops) has been seldom investigated. The aim of this study was to identify whether distinct sensitivity exists between oysters, Crassostrea gigas, and scallops, Chlamys farreri under the same amount of STX exposure using multiple biomarker responses. The responses of different biochemical markers including oxidative stress markers (catalase, superoxide dismutase, glutathione S-transferase, and lipid peroxidation) and immunotoxicity biomarkers (hemocyte phagocytosis rate, reactive oxidative species production, and DNA damages) were evaluated in bivalves after 12, 48, and 96 h of exposure to STX. The integrated biomarker responses value combined with two-way ANOVA analysis suggested that STX posed slightly severer stress on scallops than oysters for the extended period of time. This study provided preliminary results on the usefulness of a multi-biomarker approach to assess the toxicity associated with STX exposure in marine bivalves.

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“…Regarding estuarine and marine bivalve species, a question still remains whether these exhibit the same behavior and tolerance as their freshwater relatives, to feeding on toxic cyanobacteria and exposure to cyanotoxins. Studies have shown that estuarine and marine bivalve species such as Mytilus galloprovincialis and Ruditapes philippinarum modulate their detoxification and oxidative-stress defense systems after exposure to MCs [23,24]. Indeed, contamination of estuarine and coastal areas derived from the flow of contaminated freshwaters from inland to the sea have been reported in different geographical regions [25][26][27], constituting a potential thread to marine ecosystems.…”

Section: Introductionmentioning

confidence: 99%

Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

Oliveira

Díez-Quijada

Turkina

et al. 2020

Toxins

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Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by the exposure to freshwater cyanobacteria and cyanotoxins. So far, most of the knowledge has been gathered from freshwater bivalve molluscs. This work aimed to infer the sensitivity of the marine mussel Mytilus galloprovincialis to single as well as mixed toxic cyanobacterial cultures and the underlying molecular responses mediated by toxic cyanobacteria. For this purpose, a mussel exposure experiment was outlined with two toxic cyanobacteria species, Microcystis aeruginosa and Chrysosporum ovalisporum at 1 × 105 cells/mL, resembling a natural cyanobacteria bloom. The estimated amount of toxins produced by M. aeruginosa and C. ovalisporum were respectively 0.023 pg/cell of microcystin-LR (MC-LR) and 7.854 pg/cell of cylindrospermopsin (CYN). After 15 days of exposure to single and mixed cyanobacteria, a depuration phase followed, during which mussels were fed only non-toxic microalga Parachlorella kessleri. The results showed that the marine mussel is able to filter toxic cyanobacteria at a rate equal or higher than the non-toxic microalga P. kessleri. Filtration rates observed after 15 days of feeding toxic microalgae were 1773.04 mL/ind.h (for M. aeruginosa), 2151.83 mL/ind.h (for C. ovalisporum), 1673.29 mL/ind.h (for the mixture of the 2 cyanobacteria) and 2539.25 mL/ind.h (for the non-toxic P. kessleri). Filtering toxic microalgae in combination resulted in the accumulation of 14.17 ng/g dw MC-LR and 92.08 ng/g dw CYN. Other physiological and biochemical endpoints (dry weight, byssus production, total protein and glycogen) measured in this work did not change significantly in the groups exposed to toxic cyanobacteria with regard to control group, suggesting that mussels were not affected with the toxic microalgae. Nevertheless, proteomics revealed changes in metabolism of mussels related to diet, specially evident in those fed on combined cyanobacteria. Changes in metabolic pathways related with protein folding and stabilization, cytoskeleton structure, and gene transcription/translation were observed after exposure and feeding toxic cyanobacteria. These changes occur in vital metabolic processes and may contribute to protect mussels from toxic effects of the toxins MC-LR and CYN.

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Microcystin-LR bioconcentration induces antioxidant responses in the digestive gland of two marine bivalves Crassostrea gigas and Mytilus edulis

Cited by 34 publications

References 54 publications

Chlorothalonil induces oxidative stress and reduces enzymatic activities of Na+/K+-ATPase and acetylcholinesterase in gill tissues of marine bivalves

Chlorothalonil induces oxidative stress and reduces enzymatic activities of Na+/K+-ATPase and acetylcholinesterase in gill tissues of marine bivalves

Integrative Biomarker Assessment of the Influence of Saxitoxin on Marine Bivalves: A Comparative Study of the Two Bivalve Species Oysters, Crassostrea gigas, and Scallops, Chlamys farreri

Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

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