Microencapsulation of Lactobacillus casei YIT 9018 using a Microporous Glass Membrane Emulsification System

Abstract

Microporous glass (MPG) membrane emulsification was used for microencapsulation of Lactobacillus casei YIT 9018. Several process parameters of membrane emulsification were investigated for producing a stable emulsion. The droplet dia in the emulsion depended upon the membrane pore size. The monodispersed emulsion obtained by this technique resulted in well-formed microcapsules with a narrow particle size distribution. For artificial gastric acid and bile, the viable count of encapsulated cells was constant through the incubation time, while the count of nonencapsulated cells was significantly decreased. A storage stability test at different temperatures resulted in a viability of encapsulated cells 3 to 5 log cycles higher than the viability of nonencapsulated cells.

Microencapsulation of probiotic bacteria using pH-induced gelation of sodium caseinate and gellan gum

“…However, Song, Cho, and Park (2003) observed better resistance of L. casei YIT 9018 and only 4 log cfu reduction was recorded after 3 h of incubation at pH 1.2. A more pronounced lethal effect was reported by Ding and Shah (2009); when nine strains of lactobacilli and bifidobacteria were tested for acid resistance at pH 2.0, all strains were found to be badly affected, with an average log cfu reduction of 6.5e7.0 after 120 min of incubation.…”

“…As different researchers have used various concentrations and sources of bile salts, it is difficult to make any comparison with our finding. However, a lethal action of bile salts on probiotic bacteria has generally been observed (Ding & Shah, 2009;Guerin et al, 2003;Song et al, 2003). Guerin et al (2003) and Trindade and Grosso (2000) observed opposite results; free cells and encapsulated bifidobacteria cells showed higher viability after 3 h of incubation in the presence of bile salts.…”

Preparation of emulsions and particles by membrane emulsification for the food processing industry

“…Uniform emulsions and microcapsules containing viable cells (Lactobacillus casei) were produced using the membrane emulsification technique (Song et al, 2003). There has been considerable recent interest in probiotics for promotion of human health.…”

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

“…Thus, the process of particle formation has to be carefully selected. Droplet production can essentially be classified in to two types: single or series drop formation techniques (extrusion-laminar jet break-up [15]; microfluidic devices [21]), and bulk or parallel techniques (stirring [7,36]; Membrane Emulsification (ME) [20])). To-date, Song et al [20] is the only work reported for the encapsulation of cells using an SPG membrane.…”

“…Eudragit coated microparticles were produced for the colonic release of therapeutics [16,18] or proteins [19], but it has not yet been applied for the coating of encapsulated cells. A variety of materials have been tested for the encapsulation of living cells; alginate [4,6,8,12,15,[20][21][22][23] is the most common and versatile, chitosan [9,23], gelatin [22,24], cellulose [23,25], agarose [23,26], dextran [1], carrageenan [9,12,27], poly(lactide-co-glycolide) (PLGA) [1,23], Poly (Ethylene Glycol) [23,28] all have been used individually, and in blends [1,27]. Chitosan is a proven biocompatible natural polymer produced from natural sources (crustacean shells, fungi, and insects), which has been widely used for cell encapsulation and other pharmaceutical purposes [23,29].…”