Microencapsulation of lactase by W/O/W emulsion followed by complex coacervation: Effects of enzyme source, addition of potassium and core to shell ratio on encapsulation efficiency, stability and kinetics of release

Souza, Clitor J.F.; Comunian, Talita Aline; Kasemodel, Marcia Gabriela Consiglio; Favaro-Trindade, Carmen Sílvia

doi:10.1016/j.foodres.2018.12.053

Cited by 28 publications

(20 citation statements)

References 42 publications

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“…The high stability of the complex formed may be attributed to the ring structure formation, similar to that in a chelation reaction. Other factors that may be involved include electrostatic forces, van der Waals forces, and hydrogen bonding of the OH-groups of HA, although it remains difficult to determine their relative contributions [9,28,29].…”

Section: Desorption By Sodium Saltsmentioning

confidence: 99%

Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst

Ding¹,

Zhang²,

Wang³

et al. 2020

Materials

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The immobilization technology provides a potential pathway for enzyme recycling. Here, we evaluated the potential of using dextranase immobilized onto hydroxyapatite nanoparticles as a promising inorganic material. The optimal immobilization temperature, reaction time, and pH were determined to be 25 °C, 120 min, and pH 5, respectively. Dextranase could be loaded at 359.7 U/g. The immobilized dextranase was characterized by field emission gun-scanning electron microscope (FEG-SEM), X-ray diffraction (XRD), and Fourier-transformed infrared spectroscopy (FT-IR). The hydrolysis capacity of the immobilized enzyme was maintained at 71% at the 30th time of use. According to the constant temperature acceleration experiment, it was estimated that the immobilized dextranase could be stored for 99 days at 20 °C, indicating that the immobilized enzyme had good storage properties. Sodium chloride and sodium acetic did not desorb the immobilized dextranase. In contrast, dextranase was desorbed by sodium fluoride and sodium citrate. The hydrolysates were 79% oligosaccharides. The immobilized dextranase could significantly and thoroughly remove the dental plaque biofilm. Thus, immobilized dextranase has broad potential application in diverse fields in the future.

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Section: Desorption By Sodium Saltsmentioning

confidence: 99%

Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst

Ding¹,

Zhang²,

Wang³

et al. 2020

Materials

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“…The microcapsules have spherical shape, different diameters, and they are of the reservoir type, where the core is surrounded by wall material. Similar images have been reported by ; ; Souza, C. et al (2018). This demonstrated that the homogenization of the microparticles in the juice after the pasteurization did not cause major modifications or ruptures in their structures.…”

Section: Morphological Characterizationsupporting

confidence: 88%

Study of encapsulation and anticancer properties of plant extracts with probiotics

Holkem¹

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“…GM was generated through a two-step procedure. First, W/O primary emulsion was prepared according to the method described by Souza et al (2019) with slight modifications. Olive oil added with 10% (w/w) lipophilic emulsifier Span-80 was adopted as the oil fraction.…”

Section: Preparation Of Double W/o/w Emulsions and Glucoamylase Micro...mentioning

confidence: 99%

Preparation and characterization of glucoamylase microcapsules prepared by W/O/W type complex coacervation freeze drying

Zong

Tang

et al. 2022

Journal of Food Science

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Glucoamylase was often used in the brewing industry but was unstable to several environmental factors and reacted quickly to produce fermentable sugar, which limited its applications. Microencapsulation could effectively overcome the drawbacks. This study evaluated the feasibility of the preparation of glucoamylase microcapsules (GM) using W/O/W complex coacervation‐freeze‐drying method. The parameters of the microcapsules were optimized by the response surface optimization design: core‐wall ratio at 1:1, wall‐material concentration at 8%, and coagulation time for 20 min. Under current condition, the final microencapsulation efficiency reached 85.64 ± 1.60%. Glucoamylase could be slowly released for more than 96 h in the liquid state, and could react slowly for more than 336 h in the solid state. The optimized GM were incubated for 1 h, and the relative enzyme activity was better than that of free glucoamylase under high temperature conditions. The water capacity, solubility, morphology, differential scanning calorimetry, and Fourier transform infrared spectroscopy were conducted. Glucoamylase exhibited good sustained release characteristics. The microcapsules were more resistant to environmental stimuli and showed stronger robustness after optimization. Practical Application Saccharification enzymes are often used in the winemaking industry, and direct addition will cause the fermentation process to heat up too quickly, resulting in the inactivation of microorganisms and saccharification enzymes, affecting the efficiency of saccharification enzymes. Therefore, microcapsules are used to encapsulate the saccharification enzyme, and its efficacy is slowly released for a long time during the fermentation process.

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Microencapsulation of lactase by W/O/W emulsion followed by complex coacervation: Effects of enzyme source, addition of potassium and core to shell ratio on encapsulation efficiency, stability and kinetics of release

Cited by 28 publications

References 42 publications

Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst

Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst

Study of encapsulation and anticancer properties of plant extracts with probiotics

Preparation and characterization of glucoamylase microcapsules prepared by W/O/W type complex coacervation freeze drying

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