Microencapsulation to reduce mechanical loss of microspheres: implications in myocardial cell therapy

Abstract

We concluded that suboptimal functional improvement in this animal model starts at 1.5 × 10(6) cells and does not respond to escalating cell doses. Improving mechanical retention is possible by increasing the size of the injectate. Microencapsulation could be used to encapsulate donor cells and facilitate functional improvement in cellular heart failure therapy.

Mesenchymal stromal cells as universal donor cells

“…It is also important to note that there is typically a low retention yield when MSCs are directly injected within the myocardium, or given intravenously. This is mostly attributed to the mechanical loss secondary to leakage and washout or the passage through the lungs [11]. This could also partially explain the different results sometimes observed among different studies.…”

Intramyocardial sustained delivery of placental growth factor using nanoparticles as a vehicle for delivery in the rat infarct model

“…This result is comparable to the authors' previous study that examined LVFS for a control group that received only culture medium at 7 weeks. 7 This comparison proves that the nanoparticles used had no negative effect on the heart. For group II, LVFS decreased initially as a response to the MI but started to increase after 2 days till it plateaued at 1 week.…”

“…7 Briefly, rats were anesthetized using 5% isoflurane, intubated, and mechanically ventilated at 80 breaths/minute. Via a left thoracotomy (through the fourth intercostal space), the left anterior descending coronary artery was permanently ligated 2 mm from its origin with a 7/0 polypropylene suture (Ethicon Inc, Somerville, NJ).…”

“…Possible causes for this include the modes of delivery, local enzymatic degradation, differences between animal models, and the "washing-out" mechanism of the contracting myocardium. 7 One novel approach to dealing with these problems is to use nanoparticles for drug delivery. Nanodelivery systems deal with materials and systems whose structures and components exhibit novel and significantly improved physical, chemical, and biological properties due to their nanoscale size.…”

Characterization of a bioactive fiber scaffold with entrapped HUVECs in coaxial electrospun core-shell fiber

“…[1][2][3] Cells can release therapeutic proteins/ biomolecules to mitigate such diseases with delivery controlled by physiological cues via cell surface receptors. 4,5 In such systems, non-autologous cells require entrapment by a semi-permeable membrane to shield them from the immune system.…”