Microevolution of Sabin 1 Strain in Vitro and Genetic Stability of Oral Poliovirus Vaccine

Rezapkin, Gennady V.; Chumakov, Konstantin; Lu, Zhengbin; Ran, Yuxin; Dragunsky, Eugenia; Levenbook, Inessa S.

doi:10.1006/viro.1994.1353

Cited by 39 publications

(58 citation statements)

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“…The obtained nucleotide sequences were compared with those of Sabin vaccine strains determined by direct sequencing of PCR products (51,54,61). Multiple alignments of these sequences were carried out with the program CLUSTAL W, version 1.83 (62).…”

Section: Comparative Analysis Of Nucleotide and Corresponding Amino Amentioning

confidence: 99%

“…Some of the amino acid residues involved in antigenicity changes, for instance, 1090 and 1099 in type 1, 3075 and 3077 in type 2, and 3077 in type 3, do not seem to directly participate in at least the first steps of the virionreceptor interaction, as judged by crystallographic data (6,21,22). Importantly, the type 1 and type 3 Sabin-specific amino acid residues exhibiting a strong tendency to change in vaccinees appear to be quite stable upon passaging in tissue cultures (51,54), though relevant changes may take place under certain conditions (46,47). To reconcile the hypothesis on the optimization of receptor recognition as a cause of changes in the antigenic sites with the stability of these sites in tissue culture, one might consider the possibility that the requirements for optimal virion-receptor interactions in tissue culture and in gut may be different.…”

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confidence: 98%

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Antigenic Evolution of Vaccine-Derived Polioviruses: Changes in Individual Epitopes and Relative Stability of the Overall Immunological Properties

Yakovenko

Cherkasova

Rezapkin

et al. 2006

J Virol

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The Sabin oral poliovirus vaccine (OPV) readily undergoes changes in antigenic sites upon replication in humans. Here, a set of antigenically altered descendants of the three OPV serotypes (76 isolates) was characterized to determine the driving forces behind these changes and their biological implications. The amino acid residues of OPV derivatives that lie within or close to the known antigenic sites exhibited a marked tendency to be replaced by residues characteristic of homotypic wild polioviruses, and these changes may occur very early in OPV evolution. The specific amino acid alterations nicely correlated with serotype-specific changes in the reactivity of certain individual antigenic sites, as revealed by the recently devised monoclonal antibody-based enzyme-linked immunosorbent assay. In comparison to the original vaccine, small changes, if any, in the neutralizing capacity of human or rabbit sera were observed in highly diverged vaccine polioviruses of three serotypes, in spite of strong alterations of certain epitopes. We propose that the common antigenic alterations in evolving OPV strains largely reflect attempts to eliminate fitness-decreasing mutations acquired either during the original selection of the vaccine or already present in the parental strains. Variability of individual epitopes does not appear to be primarily caused by, or lead to, a significant immune evasion, enhancing only slightly, if at all, the capacity of OPV derivatives to overcome immunity in human populations. This study reveals some important patterns of poliovirus evolution and has obvious implications for the rational design of live viral vaccines.

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Section: Comparative Analysis Of Nucleotide and Corresponding Amino Amentioning

confidence: 99%

mentioning

confidence: 98%

Antigenic Evolution of Vaccine-Derived Polioviruses: Changes in Individual Epitopes and Relative Stability of the Overall Immunological Properties

Yakovenko

Cherkasova

Rezapkin

et al. 2006

J Virol

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“…The neurovirulence of the isolates was analyzed by the MAPREC test, which was used to estimate the ratio of viruses containing genes of a virulent nature in a virus population. In the case of type 1 poliovirus, the stipulated cut-off of the percentage of 480-A ϩ 525-C for passing or failing the monkey neurovirulence test is approximately 5% (24). As shown in Table 1, the 480-A ϩ 525-C content of nine strains, G3-11, G4-2, G4-12, G17-21, G18-5, G26-11, G28-3, G28-9, and G42-7, was demonstrated to be over 80%, which was almost the same level as for type 1 wild strain Mahoney.…”

Section: Resultsmentioning

confidence: 99%

“…To determine specific mutations at genome positions 480 and 525, MAPREC was performed according to the methods of Chumakov et al (5, 7) and Rezapkin et al (24). Briefly, viral RNA was extracted directly from 0.5 ml of virus suspension by phenol extraction with 1% sodium dodecyl sulfate (SDS).…”

Section: Virus Isolation Sampling Was Performed Twice Monthly Betweementioning

confidence: 99%

“…PCR amplification using sense and antisense primers as described previously (7,24) and Taq DNA polymerase (AmpliTaq; Perkin-Elmer/Cetus) was conducted so as to create recognition sites for restriction enzyme AluI (for detection of virulence-associated base A at position 480) and ScrF1 (for detection of virulence-associated base C at position 525). After treatment of the amplified DNA product with the restriction enzyme, the digested material was separated by electrophoresis in a polyacrylamide gel.…”

Section: Virus Isolation Sampling Was Performed Twice Monthly Betweementioning

confidence: 99%

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Neurovirulence of Type 1 Polioviruses Isolated from Sewage in Japan

Horie¹,

Yoshida

Matsuura

et al. 2002

Appl Environ Microbiol

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Sixteen type 1 poliovirus strains were isolated from a sewage disposal plant located downstream of the Oyabe River in Japan between October 1993 and September 1995. The isolates were intratypically differentiated as vaccine-derived strains. Neutralizing antigenicity analysis with monoclonal antibodies and estimation of neurovirulence by mutant analysis by PCR and restriction enzyme cleavage (MAPREC) were performed for 13 type 1 strains of these isolates. The isolates were classified into three groups. Group I (five strains) had a variant type of antigenicity and neurovirulent phenotype. Group II (four strains) had the vaccine type of antigenicity and neurovirulent phenotype. Group III (four strains) had the vaccine type of antigenicity and an attenuated phenotype. Furthermore, it was demonstrated that the virulent isolates were neutralized by human sera obtained after oral poliomyelitis vaccine (OPV) administration, and the sera of rats immunized with inactivated poliovirus vaccine. Although vaccination was effective against virulent polioviruses, virulent viruses will continue to exist in the environment as long as OPV is in use.

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Comparative study of poliovirus excretion after vaccination of infants with two oral polio vaccines prepared on vero cells or on primary monkey kidney cells

et al. 1997

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A comparative study was designed to assess the bioequivalence of 2 oral poliovaccines (OPV) produced on 2 different cell systems: primary monkey kidney (PMK) cells and the Vero cell line. The Vero cell line has been used to overcome the problem of obtaining a regular supply of high quality monkeys that are devoid of latent viruses. For this study, 9 children were vaccinated with PMK-OPV and 12 children with Vero-OPV. The comparison covered poliovirus excretion, reversion of polioviruses in the 5'-noncoding region, and immunogenicity. Major molecular markers in the 5'-noncoding region related to neurovirulence already had been identified at position 480 for type 1, position 481 for type 2, and position 472 for type 3 poliovirus. Two nucleic-acid based methods were designed for studying these positions: a RT-PCR followed by sequencing, which required preliminary culture and cloning; and a type-specific nested PCR followed by sequencing, which enabled direct detection and genotyping of polioviruses. Twenty-eight stool specimens were analyzed by this second method with no PCR inhibition problem. The use of Vero cell line did not modify the global pattern of poliovirus excretion, reversion frequency, or seroconversion. These results provide additional support for the use of the well-characterized Vero cell line in OPV manufacturing.

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Microevolution of Sabin 1 Strain in Vitro and Genetic Stability of Oral Poliovirus Vaccine

Cited by 39 publications

References 0 publications

Antigenic Evolution of Vaccine-Derived Polioviruses: Changes in Individual Epitopes and Relative Stability of the Overall Immunological Properties

Antigenic Evolution of Vaccine-Derived Polioviruses: Changes in Individual Epitopes and Relative Stability of the Overall Immunological Properties

Neurovirulence of Type 1 Polioviruses Isolated from Sewage in Japan

Comparative study of poliovirus excretion after vaccination of infants with two oral polio vaccines prepared on vero cells or on primary monkey kidney cells

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