Microfabrication of a biomimetic arcade-like electrospun scaffold for cartilage tissue engineering applications

Girão, André F.; Semitela, Ângela; Pereira, Alexandre Luiz; Completo, António; Marques, Paula A.A.P.

doi:10.1007/s10856-020-06407-4

Cited by 19 publications

(22 citation statements)

References 48 publications

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“…Although the small pore sizes of the electrospun meshes were insufficient to ensure cell migration through the layers, they were sufficient to assess the survivability of chondrocytes exposed to electrospraying and integration on multilayer constructions, as previously demonstrated. 27,30,31…”

Section: Discussionmentioning

confidence: 99%

Multi-layered electrospinning and electrospraying approach: Effect of polymeric supplements on chondrocyte suspension

et al. 2021

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Articular cartilage was expected to be one of the first tissues to be successfully engineered, but replicating the complex fibril architecture and the cellular distribution of the native cartilage has proven difficult. While electrospinning has been widely used to reproduce the depth-dependent fibre architecture in 3D scaffolds, the chondrocyte-controlled distribution remains an unsolved problem. To incorporate cells homogeneously through the depth of scaffolds, a combination of polymer electrospinning and cell seeding is necessary. A multi-layer approach alternating between polymer electrospinning with chondrocyte electrospraying can be a solution. Still, the success of this process is related to the survival rate of the electrosprayed chondrocytes embedded within the electrospun mesh. In this regard, the present study investigated the impact of the multi-layered process and the supplementation of the electrospray chondrocyte suspension with different concentrations of Gelatin and Alginate on the viability of electrosprayed chondrocytes embedded within a Polycaprolactone/Gelatin electrospun mesh and on the mechanical properties of the resulting meshes. The addition of Gelatin in the chondrocyte suspension did not increase significantly ( p > 0.05) the percentage of viable electrosprayed chondrocytes (25%), while 3 wt% Alginate addition led to a significant ( p < 0.05) increase in chondrocyte viability (50%) relative to the case without polymer supplement (15%). Furthermore, the addition of both polymer supplements increased the mechanical properties of the multi-layer construct. These findings imply that this multi-layered approach can be applied to cartilage TE allowing for automated chondrocyte integration during scaffolds creation.

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Section: Discussionmentioning

confidence: 99%

Multi-layered electrospinning and electrospraying approach: Effect of polymeric supplements on chondrocyte suspension

et al. 2021

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“…Natural polymers can be blended with synthetic polymers to improve mechanical strength, porous scaffolds are better for chondrocyte infiltration [39]. The knee cartilage withstands a maximum compressive force of 0.84 to 3 MPa in a person weighing 70 kg [31].…”

Section: Cartilage Tissue Engineeringmentioning

confidence: 99%

“…The use of hydrogels is popular due to their similarity between its hydrated structures and the hydrophilic environment of the cartilaginous ECM, new approach is about the combination of hydrogels with fibers as reinforcement that improves tensile strength [37], [39]. Li et.…”

Section: Cartilage Tissue Engineeringmentioning

confidence: 99%

“…The scaffolds mimicked the hydrated ECM structure of cartilage through an interconnected microporous structure, increased compressive strength (207 +/-45 kPa), that resulted good cartilage substitute due to promotes chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) [37]. These scaffolds showed improved mechanical properties in compression, and imitate EMC of cartilage both in vitro with chondrocytes and in vivo with rabbits (Table 2) [31], [37]- [39].…”

Section: Cartilage Tissue Engineeringmentioning

confidence: 99%

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Wet Electrospinning and its Applications: A Review

Suaza

Henao

2022

TecnoL.

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In wet electrospinning, a natural or synthetic polymer solution is deposited on a non-solvent liquid coagulant used as collector. This technique can create 3D nanofiber scaffolds with better properties (e.g., porosity and high surface area) than those of traditional 2D scaffolds produced by standard electrospinning. Thanks to these characteristics, wet electrospinning can be employed in a wide range of tissue engineering and industrial applications. This review aims to broaden the panorama of this technique, its possible fields of action, and its range of common materials. Moreover, we also discuss its future trends. In this study, we review papers on this method published between 2017 and 2021 to establish the state of the art of wet electrospinning and its most important applications in cardiac, cartilage, hepatic, wound dressing, skin, neural, bone, and skeletal muscle tissue engineering. Additionally, we examine its industrial applications in water purification, air filters, energy, biomedical sensors, and textiles. The main results of this review indicate that 3D scaffolds for tissue engineering applications are biocompatible; mimic the extracellular matrix (ECM); allow stem cell viability and differentiation; and have high porosity, which provides greater cell infiltration compared to 2D scaffolds. Finally, we found that, in industrial applications of wet electrospinning: (1) additives improve the performance of pure polymers; (2) the concentration of the solution influences porosity and fiber packing; (3) flow rate, voltage, and distance modify fiber morphology; (4) the surface tension of the non-solvent coagulant on which the fibers are deposited has an effect on their porosity, compaction, and mechanical properties; and (5) deposition time defines scaffold thickness.

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“…Through a layer-by-layer technique, the self-assembled NM was controlled at the molecular level. This is very different from the conventional three-dimensional (3D) print or electrospinning multilayer structure fabrication technique, which was performed at a millimeter or submillimeter levels. , We incorporated transforming growth factor β-1 (TGF-β1, a chondrogenesis growth factor) into the JBNT nanomatrix and successfully encapsulated it in the inner layers of the NM fibers (Figure b). TGF-β1 has exhibited the ability to stimulate chondrogenic differentiation and chondrocyte proliferation and functions (such as proteoglycan synthesis). , Incorporation of TGF-β1 into the inner layer can prevent TGF-β1 from leaking to undesired areas and improve its effectiveness.…”

Section: Introductionmentioning

confidence: 99%

Controlled Self-Assembly of DNA-Mimicking Nanotubes to Form a Layer-by-Layer Scaffold for Homeostatic Tissue Constructs

Zhou

Zhang

Lee

et al. 2021

ACS Appl. Mater. Interfaces

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Various biomaterial scaffolds have been developed for improving stem cell anchorage and function in tissue constructs for in vitro and in vivo uses. Growth factors are typically applied to scaffolds to mediate cell differentiation. Conventionally, growth factors are not strictly localized in the scaffolds; thus, they may leak into the surrounding environment, causing undesired side effects on tissues or cells. Hence, there is a need for improved tissue construct strategies based on highly localized drug delivery and a homeostatic microenvironment. This study developed an injectable nanomatrix (NM) scaffold with a layer-by-layer structure inside each nanosized fiber of the scaffold based on controlled selfassembly at the molecular level. The NM was hierarchically assembled from Janus base nanotubes (JBNTs), matrilin-3, and transforming growth factor β-1 (TGF-β1) via bioaffinity. JBNTs, which form the NM backbone, are novel DNA-inspired nanomaterials that mimic the natural helical nanostructures of collagens. The chondrogenic factor, TGF-β1, was enveloped in the inner layer inside the NM fibers to prevent its release. Matrilin-3 was incorporated into the outer layer to create a cartilage-mimicking microenvironment and to maintain tissue homeostasis. Interestingly, human mesenchymal stem cells (hMSCs) had a strong preference to anchor along the NM fibers and formed a localized homeostatic microenvironment. Therefore, this NM has successfully generated highly organized structures via molecular self-assembly and achieved localized drug delivery and stem cell anchorage for homeostatic tissue constructs.

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Microfabrication of a biomimetic arcade-like electrospun scaffold for cartilage tissue engineering applications

Cited by 19 publications

References 48 publications

Multi-layered electrospinning and electrospraying approach: Effect of polymeric supplements on chondrocyte suspension

Multi-layered electrospinning and electrospraying approach: Effect of polymeric supplements on chondrocyte suspension

Wet Electrospinning and its Applications: A Review

Controlled Self-Assembly of DNA-Mimicking Nanotubes to Form a Layer-by-Layer Scaffold for Homeostatic Tissue Constructs

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