The use of washing machines to wash textiles gradually breaks down synthetic fibers like polyethylene terephthalate (PET) or polyester (PES) in diverse clothing materials, a process that is growing in notoriety because it generates microplastics (MPs). In this study, we investigated the emission of microfibers, including both microplastic fibers (MPFs) and natural fibers (MFs), from top-loading washing machines. Our investigation focused on four popular textiles with prevalent weave structures (plain, satin, and twill): (i) PES, (ii) tetron cotton (TC), (iii) chief value cotton (CVC), and (iv) cotton (CO) fabrics. This study also examined the effects of textile weight and detergent dosage on MF emissions. After washing, MFs were collected through filtration, and their concentrations were determined using micro-Fourier Transform Interferometry (μFTIR). The results showed varying concentrations of MFs in the washing effluent depending on the type of textile. Specifically, CVC exhibited the highest emission at 4022 particles/L, followed by TC, PES, and CO at 2844 particles/L, 2382 particles/L, and 2279 particles/L, respectively. The hydrophobic nature of PES makes this type of textile prone to rapid degradation in detergent-rich environments, leading to high MF emissions. Additionally, the mechanical properties of textiles, such as tensile and bending strengths, may play a crucial role in the generation of MFs in washing machines. Textiles made of CO with twill weaves demonstrated superior strength and correlated with lower emissions of MFs. In comparison, textiles made of CVC and satin weave exhibited lower mechanical properties, which could explain their high emissions of MFs. Finally, the MF emissions of textiles composed of PES and TC, which are plain weaved, could be attributed to their intermediate mechanical properties compared with those of CVC and CO.