This paper experimentally investigates the acoustic properties of an orifice with bias flow under medium and high sound level excitation. Orifices with two different edge configurations were tested. The study includes a wide range of bias flow velocities, various acoustic excitation levels and different frequencies. The nonlinear acoustic scattering matrix was identified by a finely controled two-source method. Aeroacoustic modal analysis was introduced based on eigenvaluedecomposition. Acoustic properties, such as impedance, nonlinear scattering matrix and the eigenvalues were compared and discussed. Experimental results also show that bias flow makes the acoustic properties more complex compared to the no bias flow case, especially when the velocity ratio between acoustic particle velocity and mean flow velocity is near unity.