Microfluidic 3D printing polyhydroxyalkanoates-based bionic skin for wound healing

Abstract

Biomimetic scaffolds with extracellular matrix (ECM)-mimicking structure have been widely investigated in wound healing applications, while insufficient mechanical strength and limited biological activity remain major challenges. Here, we present a microfluidic 3D printing biomimetic polyhydroxyalkanoates-based scaffold with excellent mechanical properties and hierarchical porous structures for enhanced wound healing. This scaffold is composed of poly(3-hydroxybutyrate-4-hydroxybutyrate) (P34HB) and polycaprolactone (PCL), endowing it with excellent tensile strength (2.99 MPa) and degradability (80% of weight loss within 7 days). The ECM-mimicking hierarchical porous structure allows bone marrow mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs) to proliferate and adhere on the scaffolds. Besides, anisotropic composite scaffolds loaded with BMSCs and HUVECs can significantly promote re-epithelization, collagen deposition and capillary formation in rat wound defects, indicating their satisfactory in vivo tissue regenerative activity. These results indicate the feasibility of polyhydroxyalkanoates-based biomimetic scaffolds for skin repair and regeneration, which also provide a promising therapeutic strategy in diverse tissue engineering applications.

Resorbable Nanomatrices from Microbial Polyhydroxyalkanoates: Design Strategy and Characterization

“…Evaluation of the dynamics of skin defect healing using wound planimetry, histological techniques, and molecular detection of angiogenesis factors, such as inflammation, type I collagen, and keratin 10 and 14, showed more effective wound healing under all types of experimental wound dressings based on BC/P(3HB-co-4HB) compared with the control, which used a commercial wound dressing, namely VoskoPran, based on beeswax and medicinal ointments (Biotekfarm, Russia). In [ 114 ], a positive assessment was also presented for P(3HB-co-4HB) and polycaprolactone copolymers, from a mixture of which biomimetic scaffolds were obtained by microfluidic 3D printing, carrying bone marrow stem cells and endothelial cells. These tissue-engineered scaffolds had excellent mechanical properties and a hierarchical porous structure, providing accelerated wound healing and promoting re-epithelialization, collagen deposition, and capillary formation in model wound defects in laboratory rats.…”

Ultrasound‐trigged micro/nanorobots for biomedical applications

“…The MNRs actuated by bubble oscillation have excellent maneuverability and on-demand controllability. Ascribing to the tremendous development of 3D printing, which allows fabrication of nano-precision objects, [98][99][100][101][102][103][104] more and more sophisticated MNRs have merged. Whereas, the lifetime and stability of the trapped bubbles play a critical role in the prolonged propulsion of MNRs.…”

Structural and Functional Adaptive Artificial Bone: Materials, Fabrications, and Properties

“…[18][19][20] Biopolymers, including poly (L-lactide) (PLLA) and poly (ε-caprolactone) (PCL), possess good biosafety, biodegradability, and formability, but their strength is insufficient, and their degradation products are acidic, easily resulting in aseptic inflammatory reaction. [21][22][23][24][25] Until now, a single kind of material is not sufficient to meet the properties requirements of bone scaffold.…”