2016
DOI: 10.3390/mi7070123
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Microfluidic Approaches for Manipulating, Imaging, and Screening C. elegans

Abstract: The nematode C. elegans (worm) is a small invertebrate animal widely used in studies related to fundamental biological processes, disease modelling, and drug discovery. Due to their small size and transparent body, these worms are highly suitable for experimental manipulations. In recent years several microfluidic devices and platforms have been developed to accelerate worm handling, phenotypic studies and screens. Here we review major tools and briefly discuss their usage in C. elegans research.

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Cited by 58 publications
(40 citation statements)
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“…Worms that are sufficiently immobilized by anesthetics will maintain a straight body, and a cover slip can be used to gently slide over the worms until they lay on their lateral side. To optimize our methods further, microfluidic devices could be used to straighten the animals and ensure entire muscle fibres on one of the lateral sides can be visualized [80].…”
Section: Limitations Of the Current Studymentioning
confidence: 99%
“…Worms that are sufficiently immobilized by anesthetics will maintain a straight body, and a cover slip can be used to gently slide over the worms until they lay on their lateral side. To optimize our methods further, microfluidic devices could be used to straighten the animals and ensure entire muscle fibres on one of the lateral sides can be visualized [80].…”
Section: Limitations Of the Current Studymentioning
confidence: 99%
“…1, 016102 (2017) multi-step fabrication and bonding techniques and may not be practical beyond two layers of membranes. 38,41,45 The cost of consumables in the lPADs is much lower compared to those in agarose plates or polymeric microfluidic devices. For example, using wax printing, it is possible to fabricate approximately 100 paper-based lPADs (4 cm 2 of device area) in an 8.5 in.…”
Section: -6mentioning
confidence: 99%
“…35,36 In the field of neurobiology, individual worms can be rapidly immobilized in microfluidic chambers where laser nanosurgery can be performed on their axons. 37 These and many more examples [38][39][40][41] show how microfluidics can be leveraged to physically or chemically manipulate worms' microcosm, along with large-scale genetic and behavioral screening to predict the role of underlying genes affecting an observed behavior.…”
Section: Introductionmentioning
confidence: 99%
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“…[18, 19] The study of nanomaterials in C. elegans and a diversity of organisms using a collaborative (multi-center) approach can facilitate a cost-effective yet robust evaluation of the risk posed by nanomaterials in the environment and humans. [20, 21]…”
Section: Introductionmentioning
confidence: 99%