Microfluidic Chip Fabrication and Method to Detect Influenza

Abstract

Fast and effective diagnostics play an important role in controlling infectious disease by enabling effective patient management and treatment.

Current Approaches for Diagnosis of Influenza Virus Infections in Humans

“…Bhattacharyya and Klapperich fabricated a plastic microfluidic solid phase extraction device to isolate viral RNA from mammalian cells infected with the influenza A (H1N1) virus [ 37 ]. In a recent report, Klapperich’s group described a disposable microchip integrated with solid-phase extraction and RT-PCR modalities capable of extracting and amplifying influenza A RNA directly from clinical specimens in less than three hours [ 38 ]. A continuous-flow microfluidic RT-PCR chip and disposable electrical printed (DEP) chips have been employed for rapid amplification and sensing of a swine-origin influenza virus.…”

Detection of Pathogenic Microorganisms by Microfluidics Based Analytical Methods

“…Microfluidic chips integrated with PCR and sample preparation can work as point-of-care devices for rapid and accurate microbial analysis. Cao et al developed a microfluidic chip that is functionalized with SPE and reverse transcription (RT)-PCR for the detection of viruses. , The system consumed low amount of reagents, was more portable than benchtop RT-PCR, and more sensitive than rapid immunoassay tests. It was used to detect influenza A directly from clinical specimens with concentrations as low as 10 3 copies/mL in 3 h. Microfluidic chips integrated with PCR/RT-PCR could also be applied to differentiate subtypes of viruses.…”

An overview of influenza A virus detection methods: from state-of-the-art of laboratories to point-of-care strategies

“…164 Gene chip technology is a high-throughput molecular biology assay that can detect nucleic acid sequences of multiple pathogens simultaneously, including inuenza A virus. 167,168 The technology is highly sensitive and specic and can be used for rapid screening of inuenza A virus infections for pathogenic diagnosis and epidemiological surveillance. 167,169,170 Kessler et al extracted viral RNA from viral samples of patients with inuenza viruses to identify the entire microarray of single genes, which can be detected in a short period of time and completed the typing.…”

“…167,168 The technology is highly sensitive and specic and can be used for rapid screening of inuenza A virus infections for pathogenic diagnosis and epidemiological surveillance. 167,169,170 Kessler et al extracted viral RNA from viral samples of patients with inuenza viruses to identify the entire microarray of single genes, which can be detected in a short period of time and completed the typing. 171 The simplicity and high information load of microarray-based method will make it a powerful auxiliary tool for global inuenza virus surveillance.…”