Microfluidic electric parallel egg-laying assay and application to in-vivo toxicity screening of microplastics using C. elegans

Youssef, Khaled; Archonta, Daphne; Kubiseski, Terrance J.; Tandon, Anurag; Rezai, Pouya

doi:10.1016/j.scitotenv.2021.147055

Cited by 14 publications

(9 citation statements)

References 54 publications

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“…In this regard, Youssef et al examined the effects of glucose and PS MPs at concentrations of 100 mg/L and 1000 mg/L on the egg-laying of Caenorhabditis elegans (C. elegans), a worm species, using a microfluidic system. This microfluidic chip was designed with eight channels that could be monitored by means of fluorescence imaging ( Figure 4 d–f) [ 76 ]. The results showed that PS MPs at a concentration of 1000 mg/L significantly reduced the egg-laying efficiency of the worms and caused reductions in body size.…”

Section: Microfluidics As a Toxicity Screening Platform For Mpsmentioning

confidence: 99%

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Microfluidics as a Ray of Hope for Microplastic Pollution

2023

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Microplastic (MP) pollution is rising at an alarming rate, imposing overwhelming problems for the ecosystem. The impact of MPs on life and environmental cycles has already reached a point of no return; yet global awareness of this issue and regulations regarding MP exposure could change this situation in favor of human health. Detection and separation methods for different MPs need to be deployed to achieve the goal of reversing the effect of MPs. Microfluidics is a well-established technology that enables to manipulate samples in microliter volumes in an unprecedented manner. Owing to its low cost, ease of operation, and high efficiency, microfluidics holds immense potential to tackle unmet challenges in MP. In this review, conventional MP detection and separation technologies are comprehensively reviewed, along with state-of-the-art examples of microfluidic platforms. In addition, we herein denote an insight into future directions for microfluidics and how this technology would provide a more efficient solution to potentially eradicate MP pollution.

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Section: Microfluidics As a Toxicity Screening Platform For Mpsmentioning

confidence: 99%

“…( f ) The experimental process was demonstrated, and fluorescence imaging (green: GFP neurons and NW1229 worms; red: worms that had ingested MPs) was utilized. Reprinted with permission from [ 76 ]. Copyright 2021, Elsevier.…”

Section: Figurementioning

confidence: 99%

Microfluidics as a Ray of Hope for Microplastic Pollution

2023

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“…This can be advantageous in cases where multiple worms need to be imaged simultaneously, and a large field of view (FOV) is required, such as in toxicity or chemotaxis assays, or when using SWCNTs as tracking markers for C. elegans nematodes in an automated detection system. [147,[167][168][169] Detecting and imaging the DPPE-PEG-SWCNTs presented a distinct challenge. Their intake by the worms was less often detectable, since they remained as individual particles or as very small bundles in the order of 1 μm, and could only be identified with the higher magnification setups.…”

Section: In Vivo Swcnt Imagingmentioning

confidence: 99%

“…Numerous microfluidics‐based solutions were previously proposed for confining worms in a controlled space, where each platform served different purposes and varied in fabrication complexity and experimental operation. [ 136–149 ] In one well‐exploited example, the “Worm Spa” microfluidic device, was used in several applications such as surveillance of C. elegans response to environmental cues, serotonin promotion of feeding dynamics, and longitudinal imaging. [ 150–153 ]…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal Tracking of Near‐Infrared Fluorescent Single‐Walled Carbon Nanotubes inC. ElegansNematodes Confined in a Microfluidics Platform

Sharaga,

Hendler‐Neumark,

Kamber

et al. 2023

Adv Materials Technologies

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Caenorhabditis elegans (C. elegans) nematodes are a powerful model organism for diverse biological and biomedical studies, benefiting from their genetic similarities to humans, small size, and transparency. However, fluorescence imaging of C. elegans can be challenging due to the strong autofluorescence in the visible range, which obscures the signal of common fluorescent proteins or dyes. Single‐walled carbon nanotubes (SWCNTs) fluoresce in the near‐infrared (NIR) range, where there is no autofluorescence background. Herein, a platform is developed for in vivo NIR imaging of C. elegans gastrointestinal tract using biocompatible SWCNTs functionalized by single‐stranded DNA or phospholipid‐polyethylene glycol (PEG). The SWCNTs serve as fluorescent tracking probes within the worm gut, following internalization along with food intake. A microfluidic confinement device is employed to ensure an anesthetics‐free feeding environment, allowing spatiotemporal control over the SWCNTs intake imaging. Furthermore, improvements in spectral colocalization, real‐time detection of intracorporeal SWCNT dynamics, and digestive trajectory tracking are demonstrated. Owing to the unique optical properties of SWCNTs and the confinement of the worms in the microfluidics system, the proposed platform facilitates advanced in vivo imaging of C. elegans in both the visible and NIR regions, opening numerous avenues for advancing research of C. elegans and other microscopic model organisms.

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“…Microfluidic analysis is a new subject in the field of chemical analysis, medical diagnosis, safety detection, sample treatment, and micro-reaction [13][14][15][16][17][18][19][20][21]. It is a comprehensive science integrating chip manufacturing, microfluidic mechanics, material transport physics, chemical reaction dynamics, spectroscopy, and modern informatics [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Smartphone-coupled three-layered paper-based microfluidic chips demonstrating stereoscopic capillary-driven fluid transport towards colorimetric detection of pesticides

Chen

Yang

et al. 2022

Anal Bioanal Chem

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The existence of pesticide residues in the hydrosphere, biosphere, and anthroposphere can cause acute or chronic diseases and deteriorate the environment. Therefore, efficient detection of pesticide residues is of great significance to prevent food poisoning, control food pollution, and protect human lives by recognizing their distribution and concentration. Herein, a novel smartphone-coupled three-layered paper-based microfluidic chip is proposed as a facile platform to detect the pesticides. The stereoscopic capillary-driven fluid transport is enabled by the three-layered microfluidic chip configuration. The detection mechanism is based on the enzyme inhibition reaction and the chromatic reaction. The detection results are obtained by a smartphone and figured out by colorimetric quantitative analysis. Taking advantages of the above merits, we demonstrate the utilization of this smartphone-coupled three-layered paper-based microfluidic chip for the effective analysis of typical pesticides (profenofo and methomyl). The linear ranges of profenofo and methomyl are 0.27-2.1 μmol L −1 and 0.14-1.85 μmol L −1 , respectively. The corresponding limits of detection in the chips are 55 nM and 34 nM, respectively. The paper-based chips are also highly cost-effective with a total cost of 0.082 ¥ per piece. It can be anticipated that this technique will open new avenues for the mass fabrication of paper-based microfluidic chips and provide state-of-the-art methods in the field of analytical chemistry.

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Microfluidic electric parallel egg-laying assay and application to in-vivo toxicity screening of microplastics using C. elegans

Cited by 14 publications

References 54 publications

Microfluidics as a Ray of Hope for Microplastic Pollution

Microfluidics as a Ray of Hope for Microplastic Pollution

Spatiotemporal Tracking of Near‐Infrared Fluorescent Single‐Walled Carbon Nanotubes inC. ElegansNematodes Confined in a Microfluidics Platform

Smartphone-coupled three-layered paper-based microfluidic chips demonstrating stereoscopic capillary-driven fluid transport towards colorimetric detection of pesticides

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