Microfluidic enrichment of bacteria coupled to contact-free lysis on a magnetic polymer surface for downstream molecular detection

Burklund, Alison; Petryk, James D.; Hoopes, P. Jack; Zhang, John X. J.

doi:10.1063/5.0011908

Cited by 12 publications

(6 citation statements)

References 38 publications

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“…The device could effectively lyse mammalian and bacteria cells, but it does not rupture the cell walls of some intractable microorganisms, according to the results. Burklund et al demonstrated a microfluidic device, which enabled highly controlled, on-chip heating for the lysis of bacteria cells [ 98 ]. The presented chip utilized an AC external magnetic field (AMF).…”

Section: Cell Lysis Methodsmentioning

confidence: 99%

Review of Microfluidic Methods for Cellular Lysis

et al. 2021

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Cell lysis is a process in which the outer cell membrane is broken to release intracellular constituents in a way that important information about the DNA or RNA of an organism can be obtained. This article is a thorough review of reported methods for the achievement of effective cellular boundaries disintegration, together with their technological peculiarities and instrumental requirements. The different approaches are summarized in six categories: chemical, mechanical, electrical methods, thermal, laser, and other lysis methods. Based on the results derived from each of the investigated reports, we outline the advantages and disadvantages of those techniques. Although the choice of a suitable method is highly dependent on the particular requirements of the specific scientific problem, we conclude with a concise table where the benefits of every approach are compared, based on criteria such as cost, efficiency, and difficulty.

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Section: Cell Lysis Methodsmentioning

confidence: 99%

Review of Microfluidic Methods for Cellular Lysis

et al. 2021

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“…Hedde et al also showed how 3D particle sorting enables the detection and isolation of E. coli from whole blood within minutes for clinically relevant fluid volumes (1-10 mL) [92]. Other combinations of microsystems coupling high throughput bacterial immunomagnetic capture to non-contact cell lysis using an alternating current magnetic field allow bacterial detection in the range of 10 2 CFU/mL with a flow rate of 50 mL/h [95].…”

Section: Microdroplets and 3d Particle Countermentioning

confidence: 99%

Technique Evolutions for Microorganism Detection in Complex Samples: A Review

et al. 2022

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Rapid detection of microorganisms is a major challenge in the medical and industrial sectors. In a pharmaceutical laboratory, contamination of medical products may lead to severe health risks for patients, such as sepsis. In the specific case of advanced therapy medicinal products, contamination must be detected as early as possible to avoid late production stop and unnecessary costs. Unfortunately, the conventional methods used to detect microorganisms are based on time-consuming and labor-intensive approaches. Therefore, it is important to find new tools to detect microorganisms in a shorter time frame. This review sums up the current methods and represents the evolution in techniques for microorganism detection. First, there is a focus on promising ligands, such as aptamers and antimicrobial peptides, cheaper to produce and with a broader spectrum of detection. Then, we describe methods achieving low limits of detection, thanks to Raman spectroscopy or precise handling of samples through microfluids devices. The last part is dedicated to techniques in real-time, such as surface plasmon resonance, preventing the risk of contamination. Detection of pathogens in complex biological fluids remains a scientific challenge, and this review points toward important areas for future research.

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“…The limitation of magnetic nanoparticles is their slow response to external magnetic stimuli, which means that they need to form larger magnetic complexes by using high nanoparticle concentrations or have long incubation times (Burklund et al, 2020). Poncelet et al (2021) Frontiers in Bioengineering and Biotechnology frontiersin.org concentration was 10 3 CFU/ml in environmental water samples.…”

Section: Loading the Samplementioning

confidence: 99%

Advances in the use of nanomaterials for nucleic acid detection in point-of-care testing devices: A review

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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The outbreak of the coronavirus (COVID-19) has heightened awareness of the importance of quick and easy testing. The convenience, speed, and timely results from point-of-care testing (POCT) in all vitro diagnostic devices has drawn the strong interest of researchers. However, there are still many challenges in the development of POCT devices, such as the pretreatment of samples, detection sensitivity, specificity, and so on. It is anticipated that the unique properties of nanomaterials, e.g., their magnetic, optical, thermal, and electrically conductive features, will address the deficiencies that currently exist in POCT devices. In this review, we mainly analyze the work processes of POCT devices, especially in nucleic acid detection, and summarize how novel nanomaterials used in various aspects of POCT products can improve performance, with the ultimate aims of offering new ideas for the application of nanomaterials and the overall development of POCT devices.

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Microfluidic enrichment of bacteria coupled to contact-free lysis on a magnetic polymer surface for downstream molecular detection

Cited by 12 publications

References 38 publications

Review of Microfluidic Methods for Cellular Lysis

Review of Microfluidic Methods for Cellular Lysis

Technique Evolutions for Microorganism Detection in Complex Samples: A Review

Advances in the use of nanomaterials for nucleic acid detection in point-of-care testing devices: A review

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