Microfluidic fabrication of hydrogel microparticles with MOF-armoured multi-enzymes for cascade biocatalytic reactions

Abstract

Uniform hydrogel microparticles with ZIF-8 nanoparticles for molecular co-confinement of cascade enzymes are developed by microfluidics to achieve enhanced stability and reusability under harsh conditions.

Biomedically-relevant metal organic framework-hydrogel composites

“…Recently, Zhang et al , developed a simple and efficient strategy to create multi-enzyme-loaded MOF–hydrogel composite (GOx&HRP@ZIF-8/polyacrylamide) microparticles, for biocatalytic cascade reactions by the utilization of droplet microfluidic techniques. 91 Uniform polyacrylamide precursor droplets formed by microfluidics, which contain ZIF-8 particle incorporating cascade enzymes, were used as templates to create the hydrogel microparticles. The resulting MOF–hydrogel composite microparticles exhibited enhanced stability for the cascade reaction after treatment in harsh conditions, as well as good recyclability.…”

Advances in metal–organic framework-based hydrogel materials: preparation, properties and applications

“…Chu and coworkers reported an efficient strategy to prepare hydrogel particles with MOF nanoparticles for the molecular co-confinement of cascade enzymes. 327 The research group employed droplet microfluidic techniques for microparticle synthesis with controllable production of microparticles and emulsions. Precursor droplets containing MOF-armored cascade enzymes formed through microfluidics were utilized as templates to fabricate hydrogel microparticles.…”

Metal–organic gels: recent advances in their classification, characterization, and application in the pharmaceutical field

“…In the MOGs formed by MOFs and polymers, the MOF nanoparticles are trapped in the spaces between the polymer gel chains. 12 A variety of natural polymers (such as chitosan, 42 soybean hull polysaccharides, 43,44 gelatin, 45,46 silk fibroin, 47 pectin, 48,49 sodium alginate, [50][51][52] and starch 53 ), and synthetic polymers (such as carboxymethyl chitosan, 54 gelatin methacrylate, 55,56 polyacrylamide, 57,58 polyvinyl alcohol, 59 poloxamer, 60 and carboxymethylcellulose 61 ) is often used as the gelation agent (Table 1). This review further divides the synthetic strategy of these MOGs into three categories including ''MOF-first'', 62 ''polymer-first'', and ''one-pot'' (Fig.…”