Microfluidic Generation of Gradient Hydrogels to Modulate Hematopoietic Stem Cell Culture Environment

Mahadik, Bhushan; Wheeler, Tobias D.; Skertich, Luke J.; Kenis, Paul J. A.; Harley, Brendan A.C.

doi:10.1002/adhm.201300263

Cited by 99 publications

(92 citation statements)

References 63 publications

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“…Mahadik et al show a microfluidic device which creates opposing gradients of two cell types [39]. This will be a useful tool to determine for example the optimal cell ratio of niche cells and stem cells.…”

Section: Gradientsmentioning

confidence: 99%

Microfluidic 3D cell culture: from tools to tissue models

Duinen

Trietsch

Joore

et al. 2015

Current Opinion in Biotechnology

442

328

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The transition from 2D to 3D cell culture techniques is an important step in a trend towards better biomimetic tissue models. Microfluidics allows spatial control over fluids in micrometer-sized channels has become a valuable tool to further increase the physiological relevance of 3D cell culture by enabling spatially controlled co-cultures, perfusion flow and spatial control over of signaling gradients. This paper reviews most important developments in microfluidic 3D culture since 2012. Most efforts were exerted in the field of vasculature, both as a tissue on its own and as part of cancer models. We observe that the focus is shifting from tool building to implementation of specific tissue models. The next big challenge for the field is the full validation of these models and subsequently the implementation of these models in drug development pipelines of the pharmaceutical industry and ultimately in personalized medicine applications.

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Section: Gradientsmentioning

confidence: 99%

Microfluidic 3D cell culture: from tools to tissue models

Duinen

Trietsch

Joore

et al. 2015

Current Opinion in Biotechnology

442

328

View full text Add to dashboard Cite

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“…The LSK faction was collect in PBS + 25% FBS on ice and immediately used [19,29,46,[67][68][69][70].…”

Section: Hematopoietic Stem Cell Isolationmentioning

confidence: 99%

Mesenchymal stromal cell remodeling of a gelatin hydrogel microenvironment defines an artificial hematopoietic stem cell niche

Gilchrist

Lee

et al. 2018

Preprint

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We identify a combination of an initially low-diffusivity (autocrine dominated) hydrogel and a 1:1 HSCP:MSC seeding ratio as conducive to enhanced HSC population maintenance. Further, gene expression and serial mechanical testing data suggests that MSC-mediated matrix remodeling is significant for the long-term HSC culture, reducing HSC autocrine feedback and potentially enhancing MSC-mediated paracrine signaling over 7-day culture in vitro. This work demonstrates the design of an HSC culture system that couples initial hydrogel properties, MSC co-culture, and concepts of dynamic reciprocity mediated by MSC remodeling to achieve enhanced HSC maintenance.

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“…Tissue-engineered approaches to the HSC niche are frequently based on hydrogels [32,[37][38][39][40]. However, the use of solid scaffolds with higher mechanical properties allows more complex approaches.…”

Section: Discussionmentioning

confidence: 99%

Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

et al. 2017

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Endosteal-like extracellular matrix expression on melt electrospun written scaffolds, Acta Biomaterialia (2016), doi: http://dx.doi.org/10. 1016/j.actbio.2016.12.040 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. models that mimic the endosteal microenvironment enable researchers to discover the causes and improve treatments for blood and immune-related diseases. The aim of this study was to establish a physiologically relevant in vitro model using 3D printed scaffolds to assess the contribution of human cells to the formation of a construct that mimics human endosteum. Melt electrospun written scaffolds were used to compare the suitability of primary human osteoblasts (hOBs) and placenta-derived mesenchymal stem cells (plMSCs) in ( when compared to HSCs maintained using tissue culture plastic. This 3D testing platform represents an endosteal bone-like platform and warrants future investigation for the maintenance and expansion of human HSCs.

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Microfluidic Generation of Gradient Hydrogels to Modulate Hematopoietic Stem Cell Culture Environment

Cited by 99 publications

References 63 publications

Microfluidic 3D cell culture: from tools to tissue models

Microfluidic 3D cell culture: from tools to tissue models

Mesenchymal stromal cell remodeling of a gelatin hydrogel microenvironment defines an artificial hematopoietic stem cell niche

Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

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