Microfluidic image cytometry for measuring number and sizes of biological cells flowing through a microchannel using the micro-PIV technique

Hirono, Taisuke; Arimoto, Hidenobu; Okawa, Shinpei; Yamada, Yukio

doi:10.1088/0957-0233/19/2/025401

Cited by 19 publications

(14 citation statements)

References 19 publications

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“…The oneto-one correspondence of the two types of signals shows that the scattered signal is highly synchronous with the fluorescence signal. It is different from the microfluidic image cytometry (Olsen and Adrian 2000;Hirono et al 2008) using the acquired image information to determine the concentration of the particles in the suspension. However, the diameter of the scattering light detection fiber (50 lm in diameter) is only one-fourth of the fluorescence detection fiber (200 lm in diameter), the time period for a particle to pass the scatter light detection region is much shorter than that to pass the fluorescence detection region, and therefore scattered light signal response time (the width of the spikes) is shorter than fluorescence detection signal.…”

Section: Resultsmentioning

confidence: 99%

Optical microflow cytometer for particle counting, sizing and fluorescence detection

Chen

Wang

2008

Microfluid Nanofluid

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A novel on-chip optical flow cytometer concept is reported for fluorescence detection, enumeration, and sizing of microparticles in a poly-dimethylsiloxane (PDMS) microchip. The detection system integrates a pair of external optical fibers and other optical components for particle counting, sizing and fluorescence analysis in each measurement simultaneously. The scattered light signal indicates the total number and the size of the particles passing through the detection window, whereas the concurrent backward fluorescence signal shows only the number of fluorescence particles. In the experiments, microparticles of four different sizes with diameters ranging from 3.2 to 10.2 lm were discriminated and counted based on the fluorescence and scattered light intensity. The relative percentage of the fluorescence-labeled particles can be analyzed by the ratio of the events of fluorescence signals to forward scattered signals.

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Section: Resultsmentioning

confidence: 99%

Optical microflow cytometer for particle counting, sizing and fluorescence detection

Chen

Wang

2008

Microfluid Nanofluid

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“…In the present study, S is 1.0×10 −2 , much smaller than 1. This implies that the particles follow the flow substantially (Hirono et al, 2008). During the experiments, temperature and RH inside the air-conditioned room were maintained at 25°C and 45%, respectively.…”

Section: Micro-piv Techniquementioning

confidence: 99%

Effect of fluid viscosity on the liquid-feeding flow phenomena of a female mosquito

Kim

Seo

et al. 2012

Journal of Experimental Biology

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SUMMARYLiquid-sucking phenomena by the two-pump system of female mosquitoes were investigated to understand the feeding mechanism. In most previous experimental studies on liquid-feeding insects, the net increase of mass was divided by the feeding time and fluid density to evaluate the intake rate. However, this weighting method is not so precise for mosquitoes, because they are too lightweight to measure the gain of mass accurately. In this study, the intake rate of female mosquitoes feeding on various sucrose solutions was estimated using a micro-particle image velocimetry technique. As the sucrose concentration increased from 1% to 50%, the intake rate decreased from 17.3 to 5.8nls -1. In addition, the temporal volume variations of the two pump chambers were estimated based on the velocity and acceleration information of the flow at the center of the food canal of the proboscis. One pumping period was divided into four elementary phases, which are related to the different operational modes of the two pumps. According to the hypothetical model established in this study, the phase shift (α) between the two pump chambers increases from 14 to 28ms and the percentage of reverse flow to forward flow in a pumping period decreases from 7.6% to 1.7% with increasing viscosity. The developed analytical methodology thus aids in the study of an insect's feeding mechanism.

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“…Hirono group developed the image cytometry centrifugation (ICC) to count leukocytes (Yabusaki et al 1999(Yabusaki et al , 2000 and later extended their research to study theoretically and experimentally a microfluidic image cytometry by using micro-PIV flow visualization technique (Hirono et al 2008). In their research, numbers and sizes of particles flowing through a microchannel were measured simultaneously by image sequence analysis.…”

Section: Micro-piv Methodsmentioning

confidence: 99%

Methods for counting particles in microfluidic applications

Zhang

Chon

Pan

et al. 2009

Microfluid Nanofluid

122

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Microfluidic particle counters are important tools in biomedical diagnostic applications such as flow cytometry analysis. Major methods of counting particles in microfluidic devices are reviewed in this paper. The microfluidic resistive pulse sensor advances in sensitivity over the traditional Coulter counter by improving signal amplification and noise reduction techniques. Nanoporebased methods are used for single DNA molecule analysis and the capacitance counter is useful in liquids of low electrical conductivity and in sensing the changes of cell contents. Light-scattering and light-blocking counters are better for detecting larger particles or concentrated particles. Methods of using fluorescence detection have the capability for differentiating particles of similar sizes but different types that are labeled with different fluorescent dyes. The micro particle image velocimetry method has also been used for detecting and analyzing particles in a flow field. The general limitation of microfluidic particle counters is the low throughput which needs to be improved in the future. The integration of two or more existing microfluidic particle counting techniques is required for many practical on-chip applications.

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Microfluidic image cytometry for measuring number and sizes of biological cells flowing through a microchannel using the micro-PIV technique

Cited by 19 publications

References 19 publications

Optical microflow cytometer for particle counting, sizing and fluorescence detection

Optical microflow cytometer for particle counting, sizing and fluorescence detection

Effect of fluid viscosity on the liquid-feeding flow phenomena of a female mosquito

Methods for counting particles in microfluidic applications

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