Microfluidic PCR Chips

Chen, Jingdong; Chen, Di; Yuan, Tao; Chen, Xiang

doi:10.5101/nbe.v3i4.p203-210

Cited by 6 publications

(4 citation statements)

References 78 publications

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“…Microfluidics as a contemporary technology offers a multitude of application possibilities by manipulating fluids in microchannels and is becoming increasingly important not only in point-of-care testing, analysis, biotechnology, and chemistry but above all for the formulation of NP-based pharmaceuticals and personalized medicine [ 18 , 19 ]. Nowadays, reams of microfluidic chips are available with all kinds of geometries that control the fluids and the mixing of fluids either actively or in a passive manner [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Optimized Encapsulation of the FLAP/PGES-1 Inhibitor BRP-187 in PVA-Stabilized PLGA Nanoparticles Using Microfluidics

et al. 2020

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The dual inhibitor of the 5-lipoxygenase-activating protein (FLAP) and the microsomal prostaglandin E2 synthase-1 (mPGES-1), named BRP-187, represents a promising drug candidate due to its improved anti-inflammatory efficacy along with potentially reduced side effects in comparison to non-steroidal anti-inflammatory drugs (NSAIDs). However, BRP-187 is an acidic lipophilic drug and reveals only poor water solubility along with a strong tendency for plasma protein binding. Therefore, encapsulation in polymeric nanoparticles is a promising approach to enable its therapeutic use. With the aim to optimize the encapsulation of BRP-187 into poly(lactic-co-glycolic acid) (PLGA) nanoparticles, a single-phase herringbone microfluidic mixer was used for the particle preparation. Various formulation parameters, such as total flow rates, flow rate ratio, the concentration of the poly(vinyl alcohol) (PVA) as a surfactant, initial polymer concentration, as well as presence of a co-solvent on the final particle size distribution and drug loading, were screened for best particle characteristics and highest drug loading capacities. While the size of the particles remained in the targeted region between 121 and 259 nm with low polydispersities (0.05 to 0.2), large differences were found in the BRP-187 loading capacities (LC = 0.5 to 7.29%) and drug crystal formation during the various formulations.

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Section: Introductionmentioning

confidence: 99%

Optimized Encapsulation of the FLAP/PGES-1 Inhibitor BRP-187 in PVA-Stabilized PLGA Nanoparticles Using Microfluidics

et al. 2020

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“…They are significantly changing the way of processing chemical and biological analysis [5,6], cell and gene analysis [7,8], drug discovery and clinical diagnostics [9,10], material synthesis [11,12] and environmental monitoring [13,14]. Among them, the applications in biology and medicine research and related areas are almost the most extensive and profound.…”

Section: Introductionmentioning

confidence: 99%

Progress of Microfluidics for Biology and Medicine

Chen

Xie

et al. 2013

Nano-Micro Lett.

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Microfluidics has been considered as a potential technology to miniaturize the conventional equipments and technologies. It offers advantages in terms of small volume, low cost, short reaction time and highthroughput. The applications in biology and medicine research and related areas are almost the most extensive and profound. With the appropriate scale that matches the scales of cells, microfluidics is well positioned to contribute significantly to cell biology. Cell culture, fusion and apoptosis were successfully performed in microfluidics. Microfluidics provides unique opportunities for rare circulating tumor cells isolation and detection from the blood of patients, which furthers the discovery of cancer stem cell biomarkers and expands the understanding of the biology of metastasis. Nucleic acid amplification in microfluidics has extended to single-molecule, high-throughput and integration treatment in one chip. DNA computer which is based on the computational model of DNA biochemical reaction will come into practice from concept in the future. In addition, microfluidics offers a versatile platform for protein-protein interactions, protein crystallization and high-throughput screening. Although microfluidics is still in its infancy, its great potential has already been demonstrated and will provide novel solutions to the high-throughput applications.

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“…However, chemiluminescence-based detection is also known to have certain shortcomings, such as, poor selectivity and low emission intensity generated by the chemical reactions catalyzed by enzymes in small volumes, which is due to the low efficiency in transforming chemical energy into light [6]. Several strategies were developed to enhance the chemiluminescence emission of chemical reactions catalyzed by enzymes (i.e., enzymatic reactions) [7]. For example, Diaz et al have demonstrated the use of fluorescein has an enhancer on the chemiluminescence of luminol with horseradish peroxidase (HRP) [8].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of the Chemiluminescence Response of Enzymatic Reactions by Plasmonic Surfaces for Biosensing Applications

et al. 2015

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We report the enhancement of chemiluminescence response of horseradish peroxidase (HRP) in bioassays by plasmonic surfaces, which are comprised of (i) silver island films (SIFs) and (ii) metal thin films (silver, gold, copper, and nickel, 1 nm thick) deposited onto glass slides. A model bioassay, based on the interactions of avidin-modified HRP with a monolayer of biotinylated poly(ethylene-glycol)-amine, was employed to evaluate the ability of plasmonic surfaces to enhance chemiluminescence response of HRP. Chemiluminescence response of HRP in model bioassays were increased up to ~3.7-fold as compared to the control samples (i.e. glass slides without plasmonic nanoparticles), where the largest enhancement of the chemiluminescence response was observed on SIFs with high loading. These findings allowed us to demonstrate the use of SIFs (high loading) for the detection of a biologically relevant target protein (glial fibrillary acidic protein or GFAP), where the chemiluminescence response of the standard bioassay for GFAP was enhanced up to ~50% as compared to bioassay on glass slides.

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Microfluidic PCR Chips

Cited by 6 publications

References 78 publications

Optimized Encapsulation of the FLAP/PGES-1 Inhibitor BRP-187 in PVA-Stabilized PLGA Nanoparticles Using Microfluidics

Optimized Encapsulation of the FLAP/PGES-1 Inhibitor BRP-187 in PVA-Stabilized PLGA Nanoparticles Using Microfluidics

Progress of Microfluidics for Biology and Medicine

Enhancement of the Chemiluminescence Response of Enzymatic Reactions by Plasmonic Surfaces for Biosensing Applications

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