Microfluidic proportional flow controller

Prentice-Mott, Harrison; Toner, Mehmet; Irimia, Daniel

doi:10.1088/0960-1317/20/11/115020

Cited by 5 publications

(7 citation statements)

References 23 publications

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“…Biosensing surfaces functionalized with alkanethiol SAMs have been reported to suffer from non-specific binding due to non-immobilized bio receptors and imperfectly formed monolayers on the polycrystalline gold grain structure of the sensor surfaces [ 49 ]. Several techniques have been tried to control this, especially through de-activating the head group with ethanolamine-HCl [ 50 , 51 , 52 ]. Blocking with ethanolamine-HCl was conducted in our work, although non-specific binding was still observed.…”

Section: Resultsmentioning

confidence: 99%

Detection of the Inflammation Biomarker C-Reactive Protein in Serum Samples: Towards an Optimal Biosensor Formula

Fakanya

Tothill

2014

Biosensors

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The development of an electrochemical immunosensor for the biomarker, C-reactive protein (CRP), is reported in this work. CRP has been used to assess inflammation and is also used in a multi-biomarker system as a predictive biomarker for cardiovascular disease risk. A gold-based working electrode sensor was developed, and the types of electrode printing inks and ink curing techniques were then optimized. The electrodes with the best performance parameters were then employed for the construction of an immunosensor for CRP by immobilizing anti-human CRP antibody on the working electrode surface. A sandwich enzyme-linked immunosorbent assay (ELISA) was then constructed after sample addition by using anti-human CRP antibody labelled with horseradish peroxidase (HRP). The signal was generated by the addition of a mediator/substrate system comprised of 3,3,5',5'-Tetramethylbenzidine dihydrochloride (TMB) and hydrogen peroxide (H2O2). Measurements were conducted using chronoamperometry at −200 mV against an integrated Ag/AgCl reference electrode. A CRP limit of detection (LOD) of 2.2 ng·mL−1 was achieved in spiked serum samples, and performance agreement was obtained with reference to a commercial ELISA kit. The developed CRP immunosensor was able to detect a diagnostically relevant range of the biomarker in serum without the need for signal amplification using nanoparticles, paving the way for future development on a cardiac panel electrochemical point-of-care diagnostic device.

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Section: Resultsmentioning

confidence: 99%

Detection of the Inflammation Biomarker C-Reactive Protein in Serum Samples: Towards an Optimal Biosensor Formula

Fakanya

Tothill

2014

Biosensors

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“…Here A inner is the surface area of the inner beams. Figure 7 plots the modeled capacitance as a function of separation S. It also plots the inverse of C(S) − C par which compensates for the last term in (5). It is visible that after removing the parasitic terms, the inverse of the capacitance scales nearly linearly with the separation.…”

Section: Electrical Modelmentioning

confidence: 99%

A piezoelectric micro control valve with integrated capacitive sensing for ambulant blood pressure waveform monitoring

Groen¹,

Wu²,

Brookhuis³

et al. 2014

J. Micromech. Microeng.

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We have designed and characterized a MEMS microvalve with built-in capacitive displacement sensing and fitted it with a miniature piezoelectric actuator to achieve active valve control. The integrated displacement sensor enables high bandwidth proportional control of the gas flow through the valve. This is an essential requirement for non-invasive blood pressure waveform monitoring based on following the arterial pressure with a counter pressure. Using the capacitive sensor, we demonstrate negligible hysteresis in the valve control characteristics. Fabrication of the valve requires only two mask steps for deep reactive ion etching (DRIE) and one release etch.

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“…Eschewing the more common serpentine channel and parallel heater configuration, Chen et al presented a racetrack-shaped continuous flow design with tiled temperature zones, yielding 30 s/cycle and PCR in under 15 min [ 74 ], a design later parallelized for high throughput (15–46 s/cycle) [ 132 ] and integrated with purification, separation, and LIF detection functionalities [ 133 ]. In another highly integrated glass and PDMS design, Jha and team detailed a low power (< 3 W) continuous flow PCR device with electrochemical cell lysis, CE separation, and amperometric detection capabilities [ 134 ].…”

Section: Background: Thermal Cycling Technologiesmentioning

confidence: 99%

The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR

et al. 2015

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Advances in molecular biology, microfluidics, and laboratory automation continue to expand the accessibility and applicability of these methods beyond the confines of conventional, centralized laboratory facilities and into point of use roles in clinical, military, forensic, and field-deployed applications. As a result, there is a growing need to adapt the unit operations of molecular biology (e.g., aliquoting, centrifuging, mixing, and thermal cycling) to compact, portable, low-power, and automation-ready formats. Here we present one such adaptation, the rotary zone thermal cycler (RZTC), a novel wheel-based device capable of cycling up to four different fixed-temperature blocks into contact with a stationary 4-microliter capillary-bound sample to realize 1-3 second transitions with steady state heater power of less than 10 W. We demonstrate the utility of the RZTC for DNA amplification as part of a highly integrated rotary zone PCR (rzPCR) system that uses low-volume valves and syringe-based fluid handling to automate sample loading and unloading, thermal cycling, and between-run cleaning functionalities in a compact, modular form factor. In addition to characterizing the performance of the RZTC and the efficacy of different online cleaning protocols, we present preliminary results for rapid single-plex PCR, multiplex short tandem repeat (STR) amplification, and second strand cDNA synthesis.

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Microfluidic proportional flow controller

Cited by 5 publications

References 23 publications

Detection of the Inflammation Biomarker C-Reactive Protein in Serum Samples: Towards an Optimal Biosensor Formula

Detection of the Inflammation Biomarker C-Reactive Protein in Serum Samples: Towards an Optimal Biosensor Formula

A piezoelectric micro control valve with integrated capacitive sensing for ambulant blood pressure waveform monitoring

The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR

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