Microfluidic sampling system for tissue analytics

Hokkanen, Ari; Stuns, Ingmar; Schmid, Peter; Kokkonen, Annukka; Steinecker, A.; Budczies, Jan; Heimala, Päivi; Hakalahti, Leena

doi:10.1063/1.4931045

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…Kang et al, 2021 used a silicon-coated MN with Cr/Au by deposition [ 71 ]. In contrast, different types of lithography are commonly used in the case of hollow MNs [ 63 , 86 , 87 ]. Deep reactive ion etching (DRIE) and sacrificial layer sharpening are two other techniques that have been extensively researched in MNs and used in the investigated microfluidic devices [ 70 , 74 , 75 , 76 , 77 , 78 , 81 ].…”

Section: Resultsmentioning

confidence: 99%

“…The blunt-tip silicon MN chips were used to measure indicative cancer biomarkers from the tissues. This system is reported as able to be used to extract lipids from small biopsies [ 87 ].…”

Section: Resultsmentioning

confidence: 99%

“…( D ) Silicon microneedle structure showing the solvent injection and sample aspiration during microneedle extraction. Reprinted from [ 87 ]. Copyright © 2023, with permission from American Institute of Physics.…”

Section: Figurementioning

confidence: 99%

See 2 more Smart Citations

Microneedles in Advanced Microfluidic Systems: A Systematic Review throughout Lab and Organ-on-a-Chip Applications

et al. 2023

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Microneedles (MNs) have been widely used in biomedical applications for drug delivery and biomarker detection purposes. Furthermore, MNs can also be used as a stand-alone tool to be combined with microfluidic devices. For that purpose, lab- or organ-on-a-chip are being developed. This systematic review aims to summarize the most recent progress in these emerging systems, to identify their advantages and limitations, and discuss promising potential applications of MNs in microfluidics. Therefore, three databases were used to search papers of interest, and their selection was made following the guidelines for systematic reviews proposed by PRISMA. In the selected studies, the MNs type, fabrication strategy, materials, and function/application were evaluated. The literature reviewed showed that although the use of MNs for lab-on-a-chip has been more explored than for organ-on-a-chip, some recent studies have explored this applicability with great potential for the monitoring of organ models. Overall, it is shown that the presence of MNs in advanced microfluidic devices can simplify drug delivery and microinjection, as well as fluid extraction for biomarker detection by using integrated biosensors, which is a promising tool to precisely monitor, in real-time, different kinds of biomarkers in lab- and organ-on-a-chip platforms.

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Section: Resultsmentioning

confidence: 99%

“…The blunt-tip silicon MN chips were used to measure indicative cancer biomarkers from the tissues. This system is reported as able to be used to extract lipids from small biopsies [ 87 ].…”

Section: Resultsmentioning

confidence: 99%

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Microneedles in Advanced Microfluidic Systems: A Systematic Review throughout Lab and Organ-on-a-Chip Applications

et al. 2023

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“…Rapidly evolving technologies that use phloem-specific promoters to express tagged capture molecules, such as the ribosome TRAP system and live cell imaging methods, are currently the best options for sampling vascular tissues. Future methods to access the phloem translocation stream, such as the microfluidic sampling systems developed for breast cancer tissue or lab-on-a-chip organoid systems that combine tissue-specific cell growth with sampling, have the potential to revolutionize our ability to experiment with and understand phloem functions and responses during virus infection (152,153). Such next-generation sampling methods will be key to future advances in understanding how viruses usurp the vascular phloem.…”

Section: Conclusion and Future Challengesmentioning

confidence: 99%

Viral Hacks of the Plant Vasculature: The Role of Phloem Alterations in Systemic Virus Infection

et al. 2020

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For plant viruses, the ability to load into the vascular phloem and spread systemically within a host is an essential step in establishing a successful infection. However, access to the vascular phloem is highly regulated, representing a significant obstacle to virus loading, movement, and subsequent unloading into distal uninfected tissues. Recent studies indicate that during virus infection, phloem tissues are a source of significant transcriptional and translational alterations, with the number of virus-induced differentially expressed genes being four- to sixfold greater in phloem tissues than in surrounding nonphloem tissues. In addition, viruses target phloem-specific components as a means to promote their own systemic movement and disrupt host defense processes. Combined, these studies provide evidence that the vascular phloem plays a significant role in the mediation and control of host responses during infection and as such is a site of considerable modulation by the infecting virus. This review outlines the phloem responses and directed reprograming mechanisms that viruses employ to promote their movement through the vasculature. Expected final online publication date for the Annual Review of Virology, Volume 7 is September 29, 2020. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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“…Microscale and nanoscale fluidic manipulation and sampling is a general problem in many different areas, from single cell analysis , to materials synthesis , to microfluidics. , Approaches such as microfluidics generally rely upon top-down designs but can be challenging in complex environments where surface reactivity or material compatibility are concerns. Biological cells provide an alternative design motif, using bottom-up clathrin protein assembly to dynamically bud spherical vesicles from the 2D membrane for environmental sampling.…”

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confidence: 99%

Self-Assembly of Mesoscale Artificial Clathrin Mimics

et al. 2017

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Fluidic control and sampling in complex environments is an important process in biotechnology, materials synthesis, and microfluidics. An elegant solution to this problem has evolved in nature through cellular endocytosis, where the dynamic recruitment, self-assembly, and spherical budding of clathrin proteins allows cells to sample their external environment. Yet despite the importance and utility of endocytosis, artificial systems which can replicate this dynamic behavior have not been developed. Guided by clathrin's unusual structure, we created simplified metallic microparticles that capture the three-legged shape, particle curvature, and interfacial attachment characteristics of clathrin. These artificial clathrin mimics successfully recreate biomimetic analogues of clathrin's recruitment, assembly, and budding, ultimately forming extended networks at fluid interfaces and invaginating immiscible phases into spheres under external fields. Particle curvature was discovered to be a critical structural motif, greatly limiting irreversible aggregation and inducing the legs' selective tip-to-tip attraction. This architecture provides a template for a class of active self-assembly units to drive structural and dimensional transformations of liquid-liquid interfaces and microscale fluidic sampling.

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Microfluidic sampling system for tissue analytics

Cited by 6 publications

References 14 publications

Microneedles in Advanced Microfluidic Systems: A Systematic Review throughout Lab and Organ-on-a-Chip Applications

Microneedles in Advanced Microfluidic Systems: A Systematic Review throughout Lab and Organ-on-a-Chip Applications

Viral Hacks of the Plant Vasculature: The Role of Phloem Alterations in Systemic Virus Infection

Self-Assembly of Mesoscale Artificial Clathrin Mimics

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