Microfluidic sedimentation cytometer for milk quality and bovine mastitis monitoring

García-Cordero, José L.; Barrett, Louise M.; O’Kennedy, Richard; Ricco, Antonio J.

doi:10.1007/s10544-010-9459-5

Cited by 38 publications

(31 citation statements)

References 27 publications

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“…With dimensions similar to standard compact discs (CDs), centrifugal microfluidic discs are automated, disposable, and portable diagnostic tools for point of care settings (Garcia-Cordero et al 2010a;Haeberle et al 2006;Lee et al 2011;Madou et al 2006;Steigert et al 2006;Xi et al 2010). Rotor-based microfluidic systems are plastic discs that have embedded micro-features, such as channels and chambers, designed to hold and manipulate small volumes of fluids.…”

Section: Introductionmentioning

confidence: 99%

Thermo-pneumatic pumping in centrifugal microfluidic platforms

Abi-Samra

Clime

Kong

et al. 2011

Microfluid Nanofluid

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The pumping of fluids in microfluidic discs by centrifugal forces has several advantages, however, centrifugal pumping only permits unidirectional fluid flow, restricting the number of processing steps that can be integrated before fluids reach the edge of the disc. As a solution to this critical limitation, we present a novel pumping technique for the centrifugal microfluidic disc platform, termed the thermo-pneumatic pump (TPP), that enables fluids to be transferred the center of a rotating disc by the thermal expansion of air. The TPP is easy to fabricate as it is a structural feature with no moving components and thermal energy is delivered to the pump via peripheral infrared (IR) equipment, enabling pumping while the disc is in rotation. In this report, an analytical model for the operation of the TPP is presented and experimentally validated. We demonstrate that the experimental behavior of the pump agrees well with theory and that flow rates can be controlled by changing how well the pump absorbs IR energy. Overall, the TPP enables for fluids to be stored near the edge of the disc and transferred to the center on demand, offering significant advantages to the microfluidic disc platform in terms of the handling and storage of liquids.

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Section: Introductionmentioning

confidence: 99%

Thermo-pneumatic pumping in centrifugal microfluidic platforms

Abi-Samra

Clime

Kong

et al. 2011

Microfluid Nanofluid

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“…Beyond that, species-specific diseases such as bovine mastitis must be monitored, where ELISA can be used for early diagnosis [217]. One of the most recent and commercially available examples is a microfluidic sedimentation cytometer for milk quality and bovine mastitis monitoring, reported by Garcia-Cordero et al [218]. Their detection system consists of 12 independent microfluidic devices, essentially flattened funnel structures, fabricated on the footprint of a single plastic compact disc (CD).…”

Section: Examples From Veterinary Medicinementioning

confidence: 99%

Immunodiagnostics and Immunosensor Design

Gubala¹,

Klein²,

Templeton³

et al. 2016

IUPAC Standards Online

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This work compiles information on the principles of diagnostic immunochemical methods and the recent advances in this field. It presents an overview of modern techniques for the production of diagnostic antibodies, their modification with the aim of improving their diagnostic potency, the different types of immunochemical detection systems, and the increasing diagnostic applications for human health that include specific disease markers, individualized diagnosis of cancer subtypes, therapeutic and addictive drugs, food residues, and environmental contaminants. A special focus lies in novel developments of immunosensor techniques, promising approaches to miniaturized detection units and the associated microfluidic systems. The trends towards high-throughput systems, multiplexed analysis, and miniaturization of the diagnostic tools are discussed. It is also made evident that progress in the last few years has largely relied on novel chemical approaches.

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“…Flow cytometry is a powerful technique for the measurement and analysis of various characteristics of cell samples, and is used in applications ranging from immunophenotyping [1,2] to gene diagnosis [3,4], bacterial analysis [5], clinical hematology diagnosis [6][7][8], and even food industry applications [9]. Accordingly, various discrete polychromatic detection systems [10] and spectral analysis systems [11,12] have been developed in recent decades.…”

Section: Introductionmentioning

confidence: 99%

Chip-Based Cytometry Illuminated by a Blade-Shape Continuous Light for Multispectral Detection

Lin

2016

Applied Sciences

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A high performance diascopic illumination configuration is presented for the simultaneous detection of cells and particles with different sizes and different fluorescence labels in a microchannel. In the proposed approach, the cells/particles are illuminated by an objective-type dark-field condenser equipped with a low-cost tungsten light source and are then characterized by extracting the side-scatter, absorbance, and fluorescence signals from the spectra obtained by a ultraviolet-visible-near infrared (UV-Vis-NIR) spectrometer. A modified computation model is adopted to improve the capability for discriminating more fluorescence dyes simultaneously. The feasibility of the proposed detection configuration is demonstrated by counting and classifying a mixed sample of green, red, and crimson fluorescent-labeled particles and non-labeled particles with various dimensions. The suitability of the proposed system for real-world cytometry applications is then evaluated by classifying a mixed bio-sample comprising of gastric epithelial (AGS) cells stained with Trypan-blue and Erythrosin-bluish dye, respectively. The results show that the cytometer enables the efficient detection, identification, and classification of mixed bio-samples without the need for spatial filters or delicate optical components. Consequently, the proposed system has significant potential for high-performance micro-flow cytometry applications.

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Microfluidic sedimentation cytometer for milk quality and bovine mastitis monitoring

Cited by 38 publications

References 27 publications

Thermo-pneumatic pumping in centrifugal microfluidic platforms

Thermo-pneumatic pumping in centrifugal microfluidic platforms

Immunodiagnostics and Immunosensor Design

Chip-Based Cytometry Illuminated by a Blade-Shape Continuous Light for Multispectral Detection

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