Microfluidic systems for hydrodynamic trapping of cells and clusters

Luan, Qiyue; Macaraniag, Celine; Zhou, Jian; Papautsky, Ian

doi:10.1063/5.0002866

Cited by 56 publications

(37 citation statements)

References 155 publications

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“…Trapping rate is the core indicator to evaluate functional performance of microwell platform, which is mainly depending on the geometry of microwell. In principle, the optimum size of the microwell to accommodate an individual cell is about 1 of depth/diameter ratio (Luan et al 2020 ). Tang et al designed a microwell chip with 200,000 wells (depth/diameter ratio: 20 μm/25 μm = 0.8) to screening metabolically active tumor cells.…”

Section: Platforms and Integrated Systems For Single-cell Nucleic Acid Analysismentioning

confidence: 99%

Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

Liu

Dong

et al. 2021

Microfluid Nanofluid

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Single-cell nucleic acid analysis aims at discovering the genetic differences between individual cells which is well known as the cellular heterogeneity. This technology facilitates cancer diagnosis, stem cell research, immune system analysis, and other life science applications. The conventional platforms for single-cell nucleic acid analysis more rely on manual operation or bulky devices. Recently, the emerging microfluidic technology has provided a perfect platform for single-cell nucleic acid analysis with the characteristic of accurate and automatic single-cell manipulation. In this review, we briefly summarized the procedure of single-cell nucleic acid analysis including single-cell isolation, single-cell lysis, nucleic acid amplification, and genetic analysis. And then, three representative microfluidic platforms for single-cell nucleic acid analysis are concluded as valve-, microwell-, and droplet-based platforms. Furthermore, we described the state-of-the-art integrated single-cell nucleic acid analysis systems based on the three platforms. Finally, the future development and challenges of microfluidics-based single-cell nucleic acid analysis are discussed as well.

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Section: Platforms and Integrated Systems For Single-cell Nucleic Acid Analysismentioning

confidence: 99%

Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

Liu

Dong

et al. 2021

Microfluid Nanofluid

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“…Since during the baking process PC melt gradually flows into microfeatures to fill the mold shape, it may be possible to intentionally obtain rounded, partially-filled features by interrupting the process. One potential application of this is formation of microwells with rounded bottom for culture of cell spheroids or patient-derived organoids [24]. Having wells with rounded U-shaped bottom rather than the traditional flat bottom ones has the advantage of promoting cell-to-cell adhesion and formation of spherical cell aggregates (spheroids).…”

Section: Fabrication Of Rounded Featuresmentioning

confidence: 99%

“…Having wells with rounded U-shaped bottom rather than the traditional flat bottom ones has the advantage of promoting cell-to-cell adhesion and formation of spherical cell aggregates (spheroids). Previously, we had resorted to the use of 3D printing to generate U-shaped wells [24], but were limited by printer resolution (~25 µm) and stepped cross-sectional profile. Outside cell culture, rounded features can be used as micro lenses in optical applications [25].…”

Section: Fabrication Of Rounded Featuresmentioning

confidence: 99%

Polycarbonate Masters for Soft Lithography

et al. 2021

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Fabrication of microfluidic devices by soft lithography is by far the most popular approach due to its simplicity and low cost. The approach relies on casting of elastomers, such as polydimethylsiloxane (PDMS), on masters fabricated from photoresists on silicon substrates. These masters, however, can be expensive, complicated to fabricate, and fragile. Here we describe an optimized replica molding approach to preserve the original masters by heat molding of polycarbonate (PC) sheets on PDMS molds. The process is faster and simpler than previously reported methods and does not result in a loss of resolution or aspect ratio for the features. The generated PC masters were used to successfully replicate a wide range of microfluidic devices, including rectangular channels with aspect ratios from 0.025 to 7.3, large area spiral channels, and micropost arrays with 5 µm spacing. Moreover, fabrication of rounded features, such as semi-spherical microwells, was possible and easy. Quantitative analysis of the replicated features showed variability of <2%. The approach is low cost, does not require cleanroom setting or hazardous chemicals, and is rapid and simple. The fabricated masters are rigid and survive numerous replication cycles. Moreover, damaged or missing masters can be easily replaced by reproduction from previously cast PDMS replicas. All of these advantages make the PC masters highly desirable for long-term preservation of soft lithography masters for microfluidic devices.

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“…Microfluidics are techniques for devices and systems that can control small-scale fluids, and various microfluidic devices have been actively applied in that they can simulate and analyze specific biological activities in small-sized devices using a small amount of specimen [ 10 ]. As a representative example, cell sorting, counting, and trapping miniaturized using microfluids have been developed as tools to replace existing research equipment at low cost [ 11 , 12 , 13 , 14 , 15 , 16 ]. In addition, several research groups have developed microfluidic cell culture devices that mimic human organs to explore specific cellular phenomena or to analyze the effect or toxicity of drugs [ 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

A Review of Advanced Impedance Biosensors with Microfluidic Chips for Single-Cell Analysis

et al. 2021

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Electrical impedance biosensors combined with microfluidic devices can be used to analyze fundamental biological processes for high-throughput analysis at the single-cell scale. These specialized analytical tools can determine the effectiveness and toxicity of drugs with high sensitivity and demonstrate biological functions on a single-cell scale. Because the various parameters of the cells can be measured depending on methods of single-cell trapping, technological development ultimately determine the efficiency and performance of the sensors. Identifying the latest trends in single-cell trapping technologies afford opportunities such as new structural design and combination with other technologies. This will lead to more advanced applications towards improving measurement sensitivity to the desired target. In this review, we examined the basic principles of impedance sensors and their applications in various biological fields. In the next step, we introduced the latest trend of microfluidic chip technology for trapping single cells and summarized the important findings on the characteristics of single cells in impedance biosensor systems that successfully trapped single cells. This is expected to be used as a leading technology in cell biology, pathology, and pharmacological fields, promoting the further understanding of complex functions and mechanisms within individual cells with numerous data sampling and accurate analysis capabilities.

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Microfluidic systems for hydrodynamic trapping of cells and clusters

Cited by 56 publications

References 155 publications

Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

Polycarbonate Masters for Soft Lithography

A Review of Advanced Impedance Biosensors with Microfluidic Chips for Single-Cell Analysis

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