Microfluidic techniques for high throughput single cell analysis

Reece, Amy E.; Xia, Bingzhao; Jiang, Zhongliang; Noren, Benjamin; McBride, Ralph; Oakey, John

doi:10.1016/j.copbio.2016.02.015

Cited by 127 publications

(69 citation statements)

References 74 publications

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“…Generally, it can be divided into active and passive methods. Active sorting needs external forces that can be generated by optical, magnetic, electric, or acoustic fields or piezoelectric actuators, while passive separation utilizes the internal dynamics such as specific geometric structures . Here, we summarize the major sorting methods and their characteristics in microfluidic flow cytometry, as shown in Table .…”

Section: Flow Control In Microfluidic Flow Cytometrymentioning

confidence: 99%

New advances in microfluidic flow cytometry

Gong

Fan

et al. 2018

Electrophoresis

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In recent years, researchers are paying the increasing attention to the development of portable microfluidic diagnostic devices including microfluidic flow cytometry for the point‐of‐care testing. Microfluidic flow cytometry, where microfluidics and flow cytometry work together to realize novel functionalities on the microchip, provides a powerful tool for measuring the multiple characteristics of biological samples. The development of a portable, low‐cost, and compact flow cytometer can benefit the health care in underserved areas such as Africa or Asia. In this article, we review recent advancements of microfluidics including sample pumping, focusing and sorting, novel detection approaches, and data analysis in the field of flow cytometry. The challenge of microfluidic flow cytometry is also examined briefly.

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Section: Flow Control In Microfluidic Flow Cytometrymentioning

confidence: 99%

New advances in microfluidic flow cytometry

Gong

Fan

et al. 2018

Electrophoresis

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“…For most bioresearch, the primary goal of cell lysis is to conduct a subsequent omics analysis, that is why we put these two topics in the same section. Actually, microfluidic chip has had lots of applications in genomics and proteomics, here, we illustrate a few representative examples.…”

Section: Advanced Microfluidic Applicationsmentioning

confidence: 99%

Advances in Microfluidics Applied to Single Cell Operation

Zhu

Chu

Wang

2018

Biotechnology Journal

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The field of microbiology have traditionally been concerned with and focused on studies at the population level. Microfluidic platforms have emerged as important tools for biology research at a small scale, even down to a single cell level. The spatial and temporal control of cells and stimuli transported by microfluidic channels in well-designed microsystems realized the studies of specific cells in a controlled microenvironment. The true cellular physiology responses, which are obtained mostly by inference from population-level data, could be revealed in this way. Nowadays, significant applications like cell culture, analysis, sorting, genomics, and proteomics at the single cell level have been achieved in microfluidic chips. Highly integrated microfluidic systems with complete bio-analytic functions are also coming forth and of great promise for single cell related physiology, biomedical, and high throughput screening research. Herein, the leads of technologies applied to single cell operation are reviewed. Challenges and potentials of these works are also summarized, to highlight fields for further research.

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“…However, the throughput of SFL is ultimately limited by exposure area and polymerization kinetics 33,34 . Microfluidic emulsification, a two-phase microfluidic technique capable of generating monodisperse aqueous droplets in continuous oil phase has improved microgel fabrication frequency to kHz 26 , thus providing a potential tool for high throughput single cell encapsulation or screening 35-37 . Sustained cell viability following photoencapsulation is a critical parameter for therapeutic cell delivery applications.…”

Section: Introductionmentioning

confidence: 99%

A microfluidic-based cell encapsulation platform to achieve high long-term cell viability in photopolymerized PEGNB hydrogel microspheres

et al. 2017

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Cell encapsulation within photopolymerized polyethylene glycol (PEG)-based hydrogel scaffolds has been demonstrated as a robust strategy for cell delivery, tissue engineering, regenerative medicine, and developing in vitro platforms to study cellular behavior and fate. Strategies to achieve spatial and temporal control over PEG hydrogel mechanical properties, chemical functionalization, and cytocompatibility have advanced considerably in recent years. Recent microfluidic technologies have enabled the miniaturization of PEG hydrogels, thus enabling the fabrication of miniaturized cell-laden vehicles. However, rapid oxygen diffusive transport times on the microscale dramatically inhibit chain growth photopolymerization of polyethylene glycol diacrylate (PEGDA), thus decreasing the viability of cells encapsulated within these microstructures. Another promising PEG-based scaffold material, PEG norbornene (PEGNB), is formed by a step-growth photopolymerization and is not inhibited by oxygen. PEGNB has also been shown to be more cytocompatible than PEGDA and allows for orthogonal addition reactions. The step-growth kinetics, however, are slow and therefore challenging to fully polymerize within droplets flowing through microfluidic devices. Here, we describe a microfluidic-based droplet fabrication platform that generates consistently monodisperse cell-laden water-in-oil emulsions. Microfluidically generated PEGNB droplets are collected and photopolymerized under UV exposure in bulk emulsions. In this work, we compare this microfluidic-based cell encapsulation platform with a vortex-based method on the basis of microgel size, uniformity, post-encapsulation cell viability and long-term cell viability. Several factors that influence post-encapsulation cell viability were identified. Finally, long-term cell viability achieved by this platform was compared to a similar cell encapsulation platform using PEGDA. We show that this PEGNB microencapsulation platform is capable of generating cell-laden hydrogel microspheres at high rates with well-controlled size distributions and high long-term cell viability.

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Microfluidic techniques for high throughput single cell analysis

Cited by 127 publications

References 74 publications

New advances in microfluidic flow cytometry

New advances in microfluidic flow cytometry

Advances in Microfluidics Applied to Single Cell Operation

A microfluidic-based cell encapsulation platform to achieve high long-term cell viability in photopolymerized PEGNB hydrogel microspheres

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