Microfluidics for Combating Antimicrobial Resistance

Liu, Zhengzhi; Banaei, Niaz; Ren, Kangning

doi:10.1016/j.tibtech.2017.07.008

Cited by 36 publications

(35 citation statements)

References 59 publications

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“…The red colour intensity increased with time (Figure 4a), and the colour intensity levels variability was found to be 5% (%RSD) between the 15 microchannels and 9% (%RSD) between the microchips (n = 3). Figure 4c shows the graph of colour intensity change at different times (3,6,12,18,24,30, 60 and 120 s) for the 15 microchannels in a single microchip. Biosensors 2020, 10, x FOR PEER REVIEW 5 of 8 Furthermore, the presence of β-lactamase, an indicator of antimicrobial resistance [23,24] was tested by dipping nitrocefin-coated microchannels into a β-lactamase solution (Abcam, recombinant Escherichia coli β-lactamase protein) and DI water samples.…”

Section: Characterization Of Reagent Cross-linking Inside the Microchmentioning

confidence: 99%

“…The red colour intensity increased with time ( Figure 4a), and the colour intensity levels variability was found to be 5% (%RSD) between the 15 microchannels and 9% (%RSD) between the microchips (n = 3). Figure 4c shows the graph of colour intensity change at different times (3,6,12,18,24,30, 60 and 120 s) for the 15 microchannels in a single microchip. A smartphone (iPhone XR) was used to capture images of the microchip under external light, which could add variations in the light signal of the different microchannels.…”

Section: Characterization Of Reagent Cross-linking Inside the Microchmentioning

confidence: 99%

“…The reagents can be pre-coated on to the surface of the channels, and results are obtained via a portable detector/smartphone by merely dipping the device into the patient sample, after a short incubation. Therefore, this type of system can lead to the development of the ever-promised simple point-of-care (POC) devices sought after by the scientific community for decades [2,3].…”

Section: Introductionmentioning

confidence: 99%

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Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics

Hassan

Zhang

2020

Biosensors

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Point-of-care (POC) diagnostics enables the diagnosis and monitoring of patients from the clinic or their home. Ideally, POC devices should be compact, portable and operatable without the requirement of expertise or complex fluid mechanical controls. This paper showcases a chip-and-dip device, which works on the principle of capillary-driven flow microfluidics and allows analytes' detection by multiple microchannels in a single microchip via smartphone imaging. The chip-and-dip device, fabricated with inexpensive materials, works by simply dipping the reagents-coated microchip consisting of microchannels into a fluidic sample. The sample is loaded into the microchannels via capillary action and reacts with the reagents to produce a colourimetric signal. Unlike dipstick tests, this device allows the loading of bacterial/pathogenic samples for antimicrobial testing. A single device can be coated with multiple reagents, and more analytes can be detected in one sample. This platform could be used for a wide variety of assays. Here, we show the design, fabrication and working principle of the chip-and-dip flow device along with a specific application consisting in the determination of β-lactamase activity and cortisol. The simplicity, robustness and multiplexing capability of the chip-and-dip device will allow it to be used for POC diagnostics.

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Section: Characterization Of Reagent Cross-linking Inside the Microchmentioning

confidence: 99%

“…The red colour intensity increased with time ( Figure 4a), and the colour intensity levels variability was found to be 5% (%RSD) between the 15 microchannels and 9% (%RSD) between the microchips (n = 3). Figure 4c shows the graph of colour intensity change at different times (3,6,12,18,24,30, 60 and 120 s) for the 15 microchannels in a single microchip. A smartphone (iPhone XR) was used to capture images of the microchip under external light, which could add variations in the light signal of the different microchannels.…”

Section: Characterization Of Reagent Cross-linking Inside the Microchmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics

Hassan

Zhang

2020

Biosensors

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“…This information regarding the persister cells can help in the development of the new strategies for the treatment of such diseases. Micro luidic systems have also been effectively used in the screening of the individual bacterial cell for the formation of some precise chemicals and the biomechanical characteristics which might be associated with the AMR and cannot be possible using the current methods (Liu et al, 2017).…”

Section: To Get To Know More About Bacteriamentioning

confidence: 99%

A Comprehensive Review on Current Progressions in Combating The Antimicrobial Resistance

Sain¹,

Manohar²,

Gowda³

2020

ijrps

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Anti-microbial resistance (AMR) is the ability of a microbe to resist the effect of antibiotics. It has become the most important cause of concern throughout the world. AMR may develop a variety of mechanisms, including resistance, weakness or due to a certain therapeutic failure. The three major causes of resistance were identified to be over usage of anti-microbials, over-prescription, increased agricultural usage. The mechanism which leads to the development of AMR were found to be Metabolic Inactive Persister cells biofilm formation, Swarming, Obligate intracellular microbes, Reduced uptake and efflux pumps, Modification of the antibiotic binding site, Production of competitive inhibitors. Research is still being carried out and few methods successfully delay or inhibit the progress of Resistance namely: Photodynamic therapy, which works on the principle of photosensitization, results in formation of ROS which destroy pathogenic cells, Vaccines, which strengthen the defence system to fight against foreign microorganisms efficiently, Antibiotic adjuvants, work by inhibiting any kind of mechanism related to the resistance increasing the drug’s antibacterial efficiency, Nano antibiotics which acts by interfering with the mechanisms for the development of the resistance and microfluidics give a better understanding of the bacteria and its superior antimicrobial susceptibility testing. Hence, in this review the above mechanisms of antimicrobial resistance as well the latest AMR combating strategies are been discussed.

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“…The outlook of the POC devices is to provide quick analysis which can lead towards the quicker clinical decisions and hence allow the early start of treatments for patients. These earlier well-informed treatments will have better chances to improve the health of the patients as compared to the less-informed treatments while waiting for the central lab results [4,5].…”

Section: Introductionmentioning

confidence: 99%

Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels

Hassan

Zhang

2020

Micromachines

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Miniaturized quantitative assays offer multiplexing capability in a microfluidic device for high-throughput applications such as antimicrobial resistance (AMR) studies. The detection of these multiple microchannels in a single microfluidic device becomes crucial for point-of-care (POC) testing and clinical diagnostics. This paper showcases an optical flow cell for detection of parallel microchannels in a microfluidic chip. The flow cell operates by measuring the light intensity from the microchannels based on Beer-Lambert law in a linearly moving chip. While this platform could be tailored for a wide variety of applications, here we show the design, fabrication and working principle of the device. β-lactamase, an indicator of bacterial resistance to β-lactam antibiotics, especially in milk, is shown as an example. The flow cell has a small footprint and uses low-powered, low-cost components, which makes it ideally suited for use in portable devices that require multiple sample detection in a single chip.

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Microfluidics for Combating Antimicrobial Resistance

Cited by 36 publications

References 59 publications

Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics

Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics

A Comprehensive Review on Current Progressions in Combating The Antimicrobial Resistance

Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels

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