Microfluidics for the rapid detection of Escherichia coli O157:H7 using antibody-coated microspheres

Shopsin, Bo; Yu, Jiayuan; Sun, Yan; Wang, Qiao; Xu, Shengnan; Jia, Yichen; Lin, Song; Zhang, Yueying; Wang, Chen; Zhang, Yingbo; Zhang, Xiaojie

doi:10.1080/21655979.2020.1870805

Cited by 7 publications

(4 citation statements)

References 37 publications

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“…PDMS and glass were employed for the microchannel’s fabrication, using the soft lithography technique. The results were positive for the detection of E. coli bacteria with a LOD of 4.91 × 10 cfu/ μL for a 1 μL of the sample, and the analysis duration was 1.30 h [ 171 ]. Another study exploited the P-ELISA method in a paper-based microfluidic analytical device (μPADs) for the detection of various strains of E. coli in beef samples.…”

Section: Microfluidics and Locs In Food Safety Applicationsmentioning

confidence: 99%

Polymeric and Paper-Based Lab-on-a-Chip Devices in Food Safety: A Review

2023

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Food quality and safety are important to protect consumers from foodborne illnesses. Currently, laboratory scale analysis, which takes several days to complete, is the main way to ensure the absence of pathogenic microorganisms in a wide range of food products. However, new methods such as PCR, ELISA, or even accelerated plate culture tests have been proposed for the rapid detection of pathogens. Lab-on-chip (LOC) devices and microfluidics are miniaturized devices that can enable faster, easier, and at the point of interest analysis. Nowadays, methods such as PCR are often coupled with microfluidics, providing new LOC devices that can replace or complement the standard methods by offering highly sensitive, fast, and on-site analysis. This review’s objective is to present an overview of recent advances in LOCs used for the identification of the most prevalent foodborne and waterborne pathogens that put consumer health at risk. In particular, the paper is organized as follows: first, we discuss the main fabrication methods of microfluidics as well as the most popular materials used, and then we present recent literature examples for LOCs used for the detection of pathogenic bacteria found in water and other food samples. In the final section, we summarize our findings and also provide our point of view on the challenges and opportunities in the field.

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Section: Microfluidics and Locs In Food Safety Applicationsmentioning

confidence: 99%

Polymeric and Paper-Based Lab-on-a-Chip Devices in Food Safety: A Review

2023

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“…Microfluidic approaches have emerged as a way to fulfill a demand for portable and more affordable systems for biomedical purposes [30,31], offering high speed and accurate analysis, while enabling the use of small sample volumes [32]. Furthermore, they improve the target capture/detection as a result of the smaller diffusion distances and high surface-to-volume ratios, contributing to greater sensitivities [33].…”

Section: Introductionmentioning

confidence: 99%

A microfluidic platform combined with bacteriophage receptor binding proteins for multiplex detection of Escherichia coli and Pseudomonas aeruginosa in blood

Costa

Caneira²,

Chu³

et al. 2023

Sensors and Actuators B: Chemical

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“…It will represent a turning point for personalized medicine that will force a complete reassessment of antibiotherapy. Recently, the detection of E. coli was reported by high affinity sensors—such as immuno/aptasensors—that have a high specificity, but involve a more complicated protocol [ 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Proof of Concept for the Detection with Custom Printed Electrodes of Enterobactin as a Marker of Escherichia coli

Canciu

Cernat

Tertiş

et al. 2022

IJMS

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The rapid and decentralized detection of bacteria from biomedical, environmental, and food samples has the capacity to improve the conventional protocols and to change a predictable outcome. Identifying new markers and analysis methods represents an attractive strategy for the indirect but simpler and safer detection of pathogens that could replace existing methods. Enterobactin (Ent), a siderophore produced by Escherichia coli or other Gram-negative bacteria, was studied on different electrode materials to reveal its electrochemical fingerprint—very useful information towards the detection of the bacteria based on this analyte. The molecule was successfully identified in culture media samples and a future goal is the development of a rapid antibiogram. The presence of Ent was also assessed in wastewater and treated water samples collected from the municipal sewage treatment plant, groundwater, and tap water. Moreover, a custom configuration printed on a medical glove was employed to detect the target in the presence of another bacterial marker, namely pyocyanin (PyoC), that being a metabolite specific of another pathogen bacterium, namely Pseudomonas aeruginosa. Such new mobile and wearable platforms offer considerable promise for rapid low-cost on-site screening of bacterial contamination.

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Microfluidics for the rapid detection of Escherichia coli O157:H7 using antibody-coated microspheres

Cited by 7 publications

References 37 publications

Polymeric and Paper-Based Lab-on-a-Chip Devices in Food Safety: A Review

Polymeric and Paper-Based Lab-on-a-Chip Devices in Food Safety: A Review

A microfluidic platform combined with bacteriophage receptor binding proteins for multiplex detection of Escherichia coli and Pseudomonas aeruginosa in blood

Proof of Concept for the Detection with Custom Printed Electrodes of Enterobactin as a Marker of Escherichia coli

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