The Microgravity Platform (MP) of the Chinese Space Station is locked and released by Lock-or-Release (L/R) mechanism on both sides. In order to ensure the safety and reliability of the MP under the vibration environment during the rocket launch, the L/R mechanism must output the appropriate locking torque value. Based on the structural characteristics of the Scientific Experiment Cabinet (SEC), this paper proposes a method of evaluating locking torque by combining theory with experiment, and the relationship between locking force and locking torque of L/R mechanism is proved that the locking force on both sides can reach 2000 N at 25 Nm driving torque. Finally, it is verified by vibration test that the locking torque obtained by this method can effectively guarantee the safety and reliability of the MP under vibration environment.