Microgrooved collagen-based corneal scaffold for promoting collective cell migration and antifibrosis

Xiong, Sijia; Gao, Huichang; Qin, Lanfeng; Jia, Yong‐Guang; Gao, Meng; Ren, Li

doi:10.1039/c9ra04009a

Cited by 21 publications

(15 citation statements)

References 70 publications

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“…The L929 cells on the flat substrate appear in a strap-like shape, as Liu reported on a poly (vinyl alcohol)/poly (ethylene glycol) surface [ 44 ]. Cells cultured on the line array were oriented in alignment along the space formed between two struts, which was similar to the phenomena previously reported [ 45 ], appearing in a strip shape. When adhered to the ST scaffold with small pores and thin struts, the cells showed a less elongated and more spread-out microfilament cytoskeleton with many filopodia related to the strut ( Figure S2 ).…”

Section: Resultssupporting

confidence: 83%

“…between two struts, which was similar to the phenomena previously reported [45], appearing in a strip shape. When adhered to the ST scaffold with small pores and thin struts, the cells showed a less elongated and more spread-out microfilament cytoskeleton with many filopodia related to the strut (Figure S2).…”

Section: Modulation Of Cell Behavior On Different Microstructuressupporting

confidence: 90%

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Modulation of Cell Behavior by 3D Biocompatible Hydrogel Microscaffolds with Precise Configuration

et al. 2021

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Three-dimensional (3D) micronano structures have attracted much attention in tissue engineering since they can better simulate the microenvironment in vivo. Two-photon polymerization (TPP) technique provides a powerful tool for printing arbitrary 3D structures with high precision. Here, the desired 3D biocompatible hydrogel microscaffolds (3D microscaffold) with structure design referring to fibroblasts L929 have been fabricated by TPP technology, particularly considering the relative size of cell seed (cell suspension), spread cell, strut and strut spacing of scaffold. Modulation of the cell behavior has been studied by adjusting the porosity from 69.7% to 89.3%. The cell culture experiment results reveal that the obvious modulation of F-actin can be achieved by using the 3D microscaffold. Moreover, cells on 3D microscaffolds exhibit more lamellipodia than those on 2D substrates, and thus resulting in a more complicated 3D shape of single cell and increased cell surface. 3D distribution can be also achieved by employing the designed 3D microscaffold, which would effectively improve the efficiency of information exchange and material transfer. The proposed protocol enables us to better understand the cell behavior in vivo, which would provide high prospects for the further application in tissue engineering.

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Section: Resultssupporting

confidence: 83%

Section: Modulation Of Cell Behavior On Different Microstructuressupporting

confidence: 90%

Modulation of Cell Behavior by 3D Biocompatible Hydrogel Microscaffolds with Precise Configuration

et al. 2021

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“…[ 1–5 ] However, traditional approaches mainly rely on functional biomaterials seeded with cells, supplemented with bioactive factors, or in situ recruitment of cells after transplantation. [ 6–9 ] These methods are insufficient to make a finely tuned complex milieu for providing multiscale 3D architectures, such as curved cornea with crisscross internal collagenous pattern or a chamber‐shaped helical cardiac chambers.…”

Section: Introductionmentioning

confidence: 99%

Light‐Activated Decellularized Extracellular Matrix‐Based Bioinks for Volumetric Tissue Analogs at the Centimeter Scale

Kim

Kang

Cui

et al. 2021

Adv Funct Materials

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Tissue engineering requires not only tissue‐specific functionality but also a realistic scale. Decellularized extracellular matrix (dECM) is presently applied to the extrusion‐based 3D printing technology. It has demonstrated excellent efficiency as bioscaffolds that allow engineering of living constructs with elaborate microarchitectures as well as the tissue‐specific biochemical milieu of target tissues and organs. However, dECM bioinks have poor printability and physical properties, resulting in limited shape fidelity and scalability. In this study, new light‐activated dECM bioinks with ruthenium/sodium persulfate (dERS) are introduced. The materials can be polymerized via a dityrosine‐based cross‐linking system with rapid reaction kinetics and improved mechanical properties. Complicated constructs with high aspect ratios can be fabricated similar to the geometry of the desired constructs with increased shape fidelity and excellent printing versatility using dERS. Furthermore, living tissue constructs can be safely fabricated with excellent tissue regenerative capacity identical to that of pure dECM. dERS may serve as a platform for a wider biofabrication window through building complex and centimeter‐scale living constructs as well as supporting tissue‐specific performances to encapsulated cells. This capability of dERS opens new avenues for upscaling the production of hydrogel‐based constructs without additional materials and processes, applicable in tissue engineering and regenerative medicine.

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“…The drugs like steroids, hormones, antibiotics can be loaded onto the films by hydrogen bonding, covalent bonding or simply by entrapment [50]. Such films can be used for the treatment of corneal tissue infections [53], liver cancer etc. In one of the studies, the collagen films loaded with BMPs have shown direct osteoinduction without the formation of cartilage when placed in close contact with osteogenic cells [54].…”

Section: Collagen Filmsmentioning

confidence: 99%

“…Its mechanical properties can be improved by crosslinking and composting. To maintain corneal transparency, micropatterned collagen scaffolds have been reported which can regulate cellular behaviour [53]. The source and age of collagen play a significant role in the final physical properties of scaffolds like the structure, optical properties, biocompatibility, etc.…”

Section: Corneal Tissue Engineeringmentioning

confidence: 99%

A Guide to Collagen Sources, Applications and Current Advancements

Bhadra

Sakpal

Patil

et al. 2021

Systematic Bioscience and Engineering

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The increase in use of collagen products in almost every sector of utility has stipulated the rising demand for collagen. It is an essential fibrous glycoprotein, which is abundantly present in the extracellular matrix and connective tissues in many living organisms. Owing to its unique characteristics such as biocompatibility, bioavailability and weak antigenicity it has gained major interest in the food, pharmaceutical, cosmetic, biomedical and leather industries. The collagen-based composites possess an enhanced capacity to dissipate mechanical energy, strength and stiffness making it the most promising biomolecule for its multifaceted applications. Recently, synthetic biology platforms are getting wide attention for the production of non-native collagen alternatives to meet the rising demand for collagen worldwide. In the initial part, this review aims to explain different sources, structures, biosynthesis of collagen and its types followed by the emerging applications of collagen as a next-generation biomaterial in the later part. The article emphasises the synthesis of non-native collagen using novel expression systems like plant and algae in addition to bacteria and yeast with industrial consideration. In the conclusion section, challenges and opportunities of using synthetic biology tools are described.

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Microgrooved collagen-based corneal scaffold for promoting collective cell migration and antifibrosis

Abstract: Microgrooved collagen membrane can effectively promote the epithelialization of corneal epithelial cells and inhibit the fibrosis of corneal stromal cells.

Cited by 21 publications

References 70 publications

Modulation of Cell Behavior by 3D Biocompatible Hydrogel Microscaffolds with Precise Configuration

Modulation of Cell Behavior by 3D Biocompatible Hydrogel Microscaffolds with Precise Configuration

Light‐Activated Decellularized Extracellular Matrix‐Based Bioinks for Volumetric Tissue Analogs at the Centimeter Scale

A Guide to Collagen Sources, Applications and Current Advancements

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