Microgrooved fibrillar collagen membranes as scaffolds for cell support and alignment

Vernon, Robert B.; Gooden, Michel D.; Lara, Stephanie; Wight, Thomas N.

doi:10.1016/j.biomaterials.2004.08.011

Cited by 88 publications

(76 citation statements)

References 34 publications

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“…6). It has been known for some time that cell orientation can be controlled by means of a chemically patterned surface (Nakanishi et al, 2008), a grooved surface (Vernon et al, 2005), or the use of filamentous materials (Huang et al, 2006). For C2C12 myoblasts, restriction of cell migration must be moderate because numerous myoblasts must fuse to form a myotube, and thus we employed minor grooves in which cells can migrate over the grooves but align in parallel with the grooves.…”

Section: Discussionmentioning

confidence: 99%

Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability

Fujita¹,

Shimizu²,

Nagamori³

2009

Biotech & Bioengineering

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We have succeeded in fabricating a cell sheet-polymer film complex involving a temperature-sensitive polymer that has enough mechanical strength that can be manipulated even by forceps. The polymer film can be removed by lowering the temperature after transplantation, demonstrating its potential use in regenerative medicine. Recently, tissue engineering involving cell sheets was developed, tissues being fabricated by layering of these cell sheets. This technique promises high density cell packing, which is important for native cell functions, and successful heart therapy using cardiac cell sheets has been reported. On the other hand, the fabrication of a large tissue using cell sheets is difficult because of fragility of the cell sheets. Here, we have developed a novel method in which cells are attached to a temperature-sensitive poly-N-isopropylacrylamide film mixed with laminin and collagen IV, and report that the cell sheet-polymer film complex can be manipulated with forceps. A cell sheet can be removed from the polymer film by lowering the temperature after the manipulation. We have utilized this technique for the primary myocardium and fabricated a physiologically active multi-layered cardiac cell sheet. By applying a micropattern to this polymer film, we have succeeded in making a skeletal muscle cell sheet in which myotubes are oriented in the desired direction. Overall, we showed that this method is useful for cell sheet manipulation, morphogenesis, and transplantation.

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Section: Discussionmentioning

confidence: 99%

Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability

Fujita¹,

Shimizu²,

Nagamori³

2009

Biotech & Bioengineering

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“…They fabricated three scaffolds from the photo-cross-linkable elastomer poly-glycerol-sebacate, with changes in fibre alignment of nonaligned versus aligned scaffolds. 54 fabricated thin (0.1-5.3 mm) planar and tubular CMs from the air-dried hydrogels of native, fibrillar type I collagen. They showed that the planar CMs supported robust attachment, spreading, and proliferation of HDFs and human umbilical artery smooth muscle cells.…”

Section: Fibre Alignmentmentioning

confidence: 99%

“…The collagen hydrogels were air-dried into microgrooved templates and subsequently removed in the form of grooved CMs with the potential to align cells. Transformation of the fibrillar collagen hydrogels into the grooved CMs with the capacity to support cell attachment and induce cell alignment may prove to be useful for a variety of applications in tissue engineering 54 . Kapoor et al 55 have studied the role of microtopographical features on the cytomorphology, alignment, proliferation and gene expression of tenocytes.…”

Section: Fibre Alignmentmentioning

confidence: 99%

Advances in injured tendon engineering with emphasis on the role of collagen implants

Oryan¹,

Moshiri²,

Sharifi³

2012

Hard Tissue

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IntroductionTendon injuries represent a significant clinical challenge to orthopaedic surgeons and investigators. Recently, many investigators claimed the beneficial effects of tissue engineering technologies in full-thickness tendon and ligament injuries both in vivo and in vitro. In this regard, several fabrication technologies with various types of biomaterials have been specially developed in recent years. In the present review, designing, fabrication and application of tissue-engineered products accentuating the role of collagen bioimplants in tendon tissue engineering is discussed. Conclusion Tissue engineering methods have not been able to differentiate the unimodal small-sized newly generated collagen fibrils from those of multi-modally differentiated fibrillar sizes of various diameters that are present in the normal tendons of a mature animal.

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“…Microstructured grooves were used previously in 2D to direct orientation and elongation of fibroblasts [10][11][12][13]. The process was dependent upon the orientation of actin cytoskeleton [12] that also oriented along the direction of the grooves.…”

Section: Introductionmentioning

confidence: 99%

Interactive effects of surface topography and pulsatile electrical field stimulation on orientation and elongation of fibroblasts and cardiomyocytes

Au¹,

Cheng²,

Chowdhury³

et al. 2007

Biomaterials

178

166

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In contractile tissues such as myocardium, functional properties are directly related to the cellular orientation and elongation. Thus, tissue engineering of functional cardiac patches critically depends on our understanding of the interaction between multiple guidance cues such as topographical, adhesive or electrical. The main objective of this study was to determine the interactive effects of contact guidance and electrical field stimulation on elongation and orientation of fibroblasts and cardiomyocytes, major cell populations of the myocardium. Polyvinyl surfaces were abraded using lapping paper with grain size 1 to 80μm, resulting in V-shaped abrasions with the average abrasion peak-to-peak width in the range from 3 to 13μm, and the average depth in the range from 140nm to 700nm (AFM). The surfaces with abrasions 13μm wide and 700nm deep, exhibited the strongest effect on neonatal rat cardiomyocyte elongation and orientation as well as statistically significant effect on orientation of fibroblasts, thus they were utilized for electrical field stimulation. Electrical field stimulation was performed using a regime of relevance for heart tissue in vivo as well as for cardiac tissue engineering. Stimulation (square pulses, 1ms duration, 1Hz, 2.3V/cm or 4.6V/cm) was initiated 24hr after cell seeding and maintained for additional 72hr. The cover slips were positioned between the carbon rod electrodes so that the abrasions were either parallel or perpendicular to the field lines. Non-abraded surfaces were utilized as controls. Field stimulation did not affect cell viability (live/dead staining). The presence of a well developed contractile apparatus in neonatal rat cardiomyocytes (staining for cardiac Troponin I and actin filaments) was identified in the groups cultivated on abraded surfaces in the presence of field stimulation. Overall we observed that i) fibroblast and cardiomyocyte elongation on non-abraded surfaces was significantly enhanced by electrical field stimulation ii) electrical field stimulation promoted orientation of fibroblasts in the direction perpendicular to the field lines when the abrasions were also placed perpendicular to the field lines and iii) topographical cues were a significantly stronger determinant of cardiomyocyte orientation than the electrical field stimulation. The orientation and elongation response of cardiomyocytes was completely abolished by inhibition of actin polymerization (Cytochalasin D) and only partially by inhibition of phosphatidyl-inositol 3 kinase (PI3K) pathway (LY294002).

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Microgrooved fibrillar collagen membranes as scaffolds for cell support and alignment

Cited by 88 publications

References 34 publications

Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability

Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability

Advances in injured tendon engineering with emphasis on the role of collagen implants

Interactive effects of surface topography and pulsatile electrical field stimulation on orientation and elongation of fibroblasts and cardiomyocytes

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