Microgrooves Encourage Endothelial Cell Adhesion and Organization on Shape-Memory Polymer Surfaces

Govindarajan, Tina; Shandas, Robin

doi:10.1021/acsabm.8b00833

Cited by 15 publications

(11 citation statements)

References 69 publications

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“…Literature shows a large variety of ranges and architectures affecting the EC alignment. ECs have been aligned by using: nanofibrils made of 30-50 nm collagen I fibers [13]; micropatterns mostly made of fibronectin with 2.5 μm-100 μm stripes [14,15,16]; microgrooves with ridges and grooves ranging from 200 nm to 10 μm and depths of 50 nm to 5 μm [17,18,19,20,21]; and fewer topographies with sinusoidal features with 20 μm wavelength and 6.6 μm amplitude [22]. Previously, we have shown that directional gradients can be used for screening the morphological and phenotypical response of osteoblasts [23,24], adipose tissue-derived stromal cells (ASCs) [25], and myoblasts [26].…”

Section: Introductionmentioning

confidence: 99%

Topography-driven alterations in endothelial cell phenotype and contact guidance

Suarez

Ham

Brinker

et al. 2020

Heliyon

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Understanding how endothelial cell phenotype is affected by topography could improve the design of new tools for tissue engineering as many tissue engineering approaches make use of topography-mediated cell stimulation. Therefore, we cultured human pulmonary microvascular endothelial cells (ECs) on a directional topographical gradient to screen the EC vascular-like network formation and alignment response to nano to microsized topographies. The cell response was evaluated by microscopy. We found that ECs formed unstable vascular-like networks that aggregated in the smaller topographies and flat parts whereas ECs themselves aligned on the larger topographies. Subsequently, we designed a mixed topography where we could explore the network formation and proliferative properties of these ECs by live imaging for three days. Vascular-like network formation continued to be unstable on the topography and were only produced on the flat areas and a fibronectin coating did not improve the network stability. However, an instructive adipose tissue-derived stromal cell (ASC) coating provided the correct environment to sustain the vascular-like networks, which were still affected by the topography underneath. It was concluded that large microsized topographies inhibit vascular endothelial network formation but not proliferation and flat and nano/microsized topographies allow formation of early networks that can be stabilized by using an ASC instructive layer.

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Section: Introductionmentioning

confidence: 99%

Topography-driven alterations in endothelial cell phenotype and contact guidance

Suarez

Ham

Brinker

et al. 2020

Heliyon

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“…Therefore, microgrooves are widely used for cell research. [ 58–61 ] Besides, scientists have investigated dynamic wettability [ 62 ] and microoptical devices [ 63 ] on SMP microgrooves.…”

Section: Development Of Smp Micro/nanopatternsmentioning

confidence: 99%

“…Therefore, microgrooves are widely used for cell research. [58][59][60][61] Besides, scientists have investigated dynamic wettability [62] and microoptical devices [63] on SMP microgrooves. In order to explore the impact of topographic transitions on cells, Davis et al [64] first proposed a thermally responsive cell culture system based on SMP microgrooves.…”

Section: Microgroovesmentioning

confidence: 99%

“…Shape memory micro/nanopatterns have greatly promoted the development and application of polymer materials. This review mainly introduces various shapes of SMP micro/ [58][59][60][61][62][63][64][65][66][67]94,112] Array of micropillars Acrylate-based SMP, PCL, PDLLA, epoxy-based SMP, hydrogel Thermal-induced, magnetic induced, light-induced Replica molding, thermalembossing, laser ablation Biomedicine/smart wettability/ microoptics/smart adhesive [29,30,63,[68][69][70][71][72][73]95,96,99,113,117,118,[121][122][123][124][125][126][127][128][129][130][131][132][133]142,144] Microwells PCL, PCO Thermal-induced Thermal-embossing, breath figure…”

Section: Development and Challengesmentioning

confidence: 99%

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Application and Development of Shape Memory Micro/Nano Patterns

Liu

Chen

et al. 2021

Small

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Shape memory polymers (SMPs) are a class of smart materials that change shape when stimulated by environmental stimuli. Different from the shape memory effect at the macro level, the introduction of micro‐patterning technology into SMPs strengthens the exploration of the shape memory effect at the micro/nano level. The emergence of shape memory micro/nano patterns provides a new direction for the future development of smart polymers, and their applications in the fields of biomedicine/textile/micro‐optics/adhesives show huge potential. In this review, the authors introduce the types of shape memory micro/nano patterns, summarize the preparation methods, then explore the imminent and potential applications in various fields. In the end, their shortcomings and future development direction are also proposed.

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“…[ 174 ] Similar results were discovered on polyurethane‐based shape memory polymers (SMPs) where microgrooves were found to increase surface hydrophobicity which helped to align endothelial cells along the microchannels. [ 175 ] PIII is another surface modification technique where ion‐induced carbonization and oxidation helps to covalently immobilize biomolecules to the surface. [ 176 ] PIII applied to polyurethane enhanced surface wettability and promoted saturation of collagen attachment.…”

Section: Bare or Surface‐modified Stentsmentioning

confidence: 99%

Designing Better Cardiovascular Stent Materials: A Learning Curve

Cockerill

See

Young

et al. 2020

Adv Funct Materials

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Cardiovascular stents are life‐saving devices and one of the top ten medical breakthroughs of the 21st century. Decades of research and clinical trials have taught us about the effects of material (metal or polymer), design (geometry, strut thickness, and the number of connectors), and drug‐elution on vasculature mechanics, hemocompatibility, biocompatibility, and patient health. Recently developed novel bioresorbable stents are intended to overcome common issues of chronic inflammation, in‐stent restenosis, and stent thrombosis associated with permanent stents, but there is still much to learn. Increased knowledge and advanced methods in material processing have led to new stent formulations aimed at improving the performance of their predecessors but often comes with potential tradeoffs. This review aims to discuss the advantages and disadvantages of stent material interactions with the host within five areas of contrasting characteristics, such as 1) metal or polymer, 2) bioresorbable or permanent, 3) drug elution or no drug elution, 4) bare or surface‐modified, and 5) self‐expanding or balloon‐expanding perspectives, as they relate to pre‐clinical and clinical outcomes and concludes with directions for future studies.

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Microgrooves Encourage Endothelial Cell Adhesion and Organization on Shape-Memory Polymer Surfaces

Cited by 15 publications

References 69 publications

Topography-driven alterations in endothelial cell phenotype and contact guidance

Topography-driven alterations in endothelial cell phenotype and contact guidance

Application and Development of Shape Memory Micro/Nano Patterns

Designing Better Cardiovascular Stent Materials: A Learning Curve

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