Microliter ultrafast centrifuge platform for size-based particle and cell separation and extraction using novel omnidirectional spiral surface acoustic waves

Zhang, Naiqing; Zuniga-Hertz, Juan Pablo; Zhang, Elizabeth Yan; Gopesh, Tilvawala; Fannon, McKenzie J.; Wang, Jiaying; Wen, Yue; Patel, Hemal H.; Friend, James

doi:10.1039/d0lc01012j

Cited by 45 publications

(32 citation statements)

References 40 publications

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“…On the other side, surface acoustic wave (SAW) actuators have been extensively studied for manipulation of liquid particularly with volumes of microlitres or even smaller [37][38][39] . SAW devices are commonly composed of interdigitated electrodes that are patterned on a piezoelectric substrate.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, surface acoustic wave (SAW) actuators have been extensively studied for the manipulation of liquids particularly with volumes of microlitres or even smaller. [37][38][39] SAW devices are commonly composed of interdigitated electrodes that are patterned on a piezoelectric substrate. They have been widely used in acoustofluidics to manipulate droplets for sample preparation, sorting, patterning, mixing, pumping, jetting, and nebulisation.…”

Section: Introductionmentioning

confidence: 99%

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An integrated platform for metamaterial-based sensing and surface acoustic wave-based acoustofluidics utilising circular interdigital transducers

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An integrated platform for metamaterial-based sensing and surface acoustic wave-based acoustofluidics utilising circular interdigital transducers

et al. 2022

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“…Surface acoustic waves in the frequency range of 10's MHz to 10's GHz [150,[197][198][199][200][201] have gained increased attention due to their extensive value in manipulation, separation, and concentration of organisms, [202][203][204] cells, [205][206][207][208][209][210][211][212][213][214][215][216][217][218] particles, [140,212,215,[219][220][221][222][223][224][225][226][227][228][229] and nanomaterials. [230][231][232] Recently, surface acoustic waves have been leveraged to develop various technologies for sonoporation.…”

Section: High-frequency Surface Acoustic Wave-based Technologiesmentioning

confidence: 99%

Sonoporation: Past, Present, and Future

Rich

Tian

Huang

2021

Adv Materials Technologies

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only contribute to fundamental studies in areas of biology, bioengineering, and medicine, but also benefit several life-changing technologies, such as cell-based therapies, protein therapeutics, vaccines, as well as the reprogramming of cells. [22] Among the current delivery approaches, the viral vector-based approach is often considered the gold standard for the delivery of nucleic acids. [22] However, due to notable weaknesses such as biohazards, inconsistent results, and labor-intensive manufacturing, [22][23][24] physical permeabilization approaches are being pursued, [1][2][3][4][5][6][7][8][9][10][11][12]22] including electroporation, [25,26] hydroporation, [27][28][29][30][31][32] mechanoporation, [25,[32][33][34][35][36][37][38][39][40] and sonoporation. [41,42] These approaches are noted as advanced due to their high throughputs, cost-effectiveness, and universal nature to deliver a variety of cargos regardless of cell type. [3] Of the physical permeabilization approaches, sonoporation wields great potential and has been demonstrated as an efficacious technology for delivering a variety of functional cargos, such as biomolecular drugs, proteins, and plasmids, to different types of cells, such as cancer, immune, and stem cells. [43][44][45][46][47][48] Sonoporation was discovered in the 1980s, a golden age of intracellular delivery, in which quite a few physical permeabilization methods were invented. [49][50][51][52][53][54][55][56][57][58] Sonoporation enhances the intracellular delivery of cargos into cells by employing acoustic waves to disrupt their membranes. [22,[59][60][61][62] Traditional sonoporation approaches typically rely on ultrasound-induced bubble dynamic behaviors, such as inertial cavitation, noninertial (stable) cavitation, and bubble translation. [49][50][51][52] However, the bubble-based sonoporation approaches usually require special contrast agents, [63][64][65][66][67][68] which adds a new dimension of complexity and cost to the use of sonoporation. Moreover, bubble-based approaches have a high chance of inducing irreversible cell damage, lowering the cell viability, as well as reducing the effectiveness of delivered cargos. [69][70][71][72][73] Therefore, several novel non-bubble-based sonoporation approaches, such as those based on acoustic radiation force and acoustic streaming-induced shear force, are being developed to overcome the limitations of bubble-based sonoporation approaches. Although there are several review papers on the mechanisms and applications of bubble-based sonoporation, [61,62,[74][75][76][77][78][79][80][81][82][83][84][85][86][87][88] they have neglected some of the newly developed mechanisms. Therefore, this review article covers recent innovations in bubble-based and especially in non-bubble-based sonoporation. The following sections review the sonoporation mechanisms, A surge of research in intracellular delivery technologies is underway with the increased innovations in cell-based therapies and cell reprogramming. Particularly, physical cell membrane p...

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“…Shilton et al 32 proposed to enrich particles using focused SAWs rapidly. Instead of straight IDTs, Zhang et al 33 designed an omnidirectional spiral SAW device and achieved particle enrichment utilizing the induced planar recirculating acoustic streaming in a droplet. Gu et al 20 demonstrated the enrichment and separation of nanoparticles in 1 min using the entanglement of the SAW actuation and spinning droplet.…”

Section: Introductionmentioning

confidence: 99%

Rapid Enrichment of Submicron Particles within a Spinning Droplet Driven by a Unidirectional Acoustic Transducer

et al. 2021

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Efficient and rapid particle enrichment at the submicron scale is essential for research in biomedicine and biochemistry. Here, we demonstrate an acoustofluidic method for submicron particle enrichment within a spinning droplet driven by a unidirectional transducer. The unidirectional transducer generates intense sound energy with relatively low attenuation. Droplets placed offset in the wave propagation path on a polydimethylsiloxane film undergo strong pressure gradients, deforming into an ellipsoid shape and spinning at high speed. Benefitting from the drag force induced by the droplet spin and acoustic streaming and the radial force induced by the droplet compression and expansion, the submicron particles in the liquid droplet quickly enrich toward the central area following a spiral trajectory. Through numerical calculations and experimental processes, we have demonstrated the possible mechanism responsible for particle enrichment. The application of biological sample processing has also been exploited. This study anticipates that the strategy based on the spinning droplet and particle enrichment method will be highly desirable for many applications.

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Microliter ultrafast centrifuge platform for size-based particle and cell separation and extraction using novel omnidirectional spiral surface acoustic waves

Abstract: Acoustically centrifuge and extract cells from blood samples using 152-Y-rotated lithium niobate and spiral electrodes with an extraction syringe.

Cited by 45 publications

References 40 publications

An integrated platform for metamaterial-based sensing and surface acoustic wave-based acoustofluidics utilising circular interdigital transducers

An integrated platform for metamaterial-based sensing and surface acoustic wave-based acoustofluidics utilising circular interdigital transducers

Sonoporation: Past, Present, and Future

Rapid Enrichment of Submicron Particles within a Spinning Droplet Driven by a Unidirectional Acoustic Transducer

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