Micromechanical properties of single crystal hydroxyapatite by nanoindentation

Saber‐Samandari, Saeed; Gross, Kārlis Agris

doi:10.1016/j.actbio.2009.02.009

Cited by 131 publications

(70 citation statements)

References 36 publications

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“…[15] Critically, how cells sense and respond to the mechanical properties of their surroundings in a heterogeneous environment and the role of mechanical heterogeneity in mediating skeletal stem cell function remains poorly understood. [4,16] Elastomeric substrates and hydrogels have been used to present cells with surfaces of specific stiffness, approximating the range of rigidities encountered in physiological Environments.…”

Section: Introductionmentioning

confidence: 99%

The Functional Response of Mesenchymal Stem Cells to Electron‐Beam Patterned Elastomeric Surfaces Presenting Micrometer to Nanoscale Heterogeneous Rigidity

et al. 2017

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Biggs, M. J. P. et al. (2017) The functional response of mesenchymal stem cells to electron-beam patterned elastomeric surfaces presenting micrometer to nanoscale heterogeneous rigidity. Advanced Materials, 29(39), 1702119.There may be differences between this version and the published version. You are advised to consult the publisher's version if you wish to cite from it.Biggs, M. J.P. et al. (2017) AbstractCells directly probe and respond to the physicomechanical properties of their extracellular environment, a dynamic process which has been shown to play a key role in regulating both cellular adhesive processes and differential cellular function. Recent studies indicate that stem cells show lineage-specific differentiation when cultured on substrates approximating the stiffness profiles of specific tissues. Although tissues are associated with ranging Young's modulus values for bulk rigidity, at the sub-cellular level, and particularly at the micro-and nanoscales, tissues are comprised of heterogeneous distributions of rigidity.Lithographic processes have been widely explored in cell biology for the generation of analytical substrates to probe cellular physicomechanical responses. In this work, we show for the first time that that direct-write e-beam exposure can significantly alter the rigidity of elastomeric PDMS substrates and develop a new class of two-dimensional elastomeric substrates with controlled patterned rigidity ranging from the micron to the nanoscale. The mechano-response of human mesenchymal stem cells to e-beam patterned substrates was subsequently probed in vitro and significant modulation of focal adhesion formation and osteochondral lineage commitment was observed as a function of both feature diameter and rigidity, establishing the groundwork for a new generation of biomimetic material interfaces.3

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Section: Introductionmentioning

confidence: 99%

The Functional Response of Mesenchymal Stem Cells to Electron‐Beam Patterned Elastomeric Surfaces Presenting Micrometer to Nanoscale Heterogeneous Rigidity

et al. 2017

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“…At macroscale, conventional testing [2][3][4]6,7,9,10,16,[25][26][27][28][29], but also ultrasounds [12,30] have been used to characterize cortical and trabecular tissues, showing a clear anisotropy of bone; At microscale, Ascenzi and co-workers [31][32][33][34][35] other authors used micromechanical testing [7,36,37] and acoustic microscopy [15,38,39] to evaluate the elastic properties of bone at the microstructural level; At nanoscale, the most common technique used to characterize bone tissue is nanoindentation [13][14][15]40,41]. Moreover, novel less invasive methodologies for in vivo measuring fracture toughness on small animals are of great clinical relevance, since they can be helpful in understanding the material properties of bone during preclinical testing for reducing fracture risks [42]; At nano-to subnanoscale, numerical simulations -from atomistic to coarse grain -allow researchers to examine the small scale chemo-mechanical behavior, studying characteristic phenomena, which are difficult to be reached by experiments [43].…”

Section: Introductionmentioning

confidence: 99%

Understanding the structure–property relationship in cortical bone to design a biomimetic composite

Libonati

Vergani

2016

Composite Structures

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“…In the present study as a calcium precursor Ca(CH 3 COO) 2 During the HA synthesis, biomimetic method and physiologic conditions (7.4 pH and 37…”

Section: Introductionmentioning

confidence: 99%

“…Hydroxyapatite [Ca 10 (PO 4 ) 6 (OH) 2 , HA] has been used for decades as a biomaterial in orthopedic and dental applications due to its biocompatibility and similarity to the inorganic components of bone and teeth in human [1]. Apatite used in biomedical applications mostly has a µm-or nanometer-size.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Different Phosphorus Precursors on Biomimetic Hydroxyapatite Powder Properties

et al. 2013

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Hydroxyapatite is the main component of natural hard tissues, such as teeth and bone. It has been studied extensively as a candidate biomaterial for its use in prosthetic applications. Hydroxyapatite was formulated as Ca10(PO4)6(OH)2 and it has a high stability under physiological conditions. Also hydroxyapatite can be synthesized using dierent calcium and phosphorus precursors. In this study, biomimetic hydroxyapatite powder has been synthesized simulating physiological conditions. Synthetic body uids which have the same composition as human blood plasma instead of pure water were used as precipitation media to obtain biological conditions. Recent research involved the eect of dierent Ca-precursors however aim of this study is to determine the eect of phosphorus resources. In this study, the synthesis of hydroxyapatite powder is carried out by using biomimetic method in synthetic body uids. OPA] were used as Ca-and P-precursors. Chemical structures of synthesized powders have been examined by Fourier transform infrared and X-ray diraction. Results showed that synthesized powders have a pure hydroxyapatite structure. However, ADHP precursors have an unfavorable eect on sintered hydroxyapatite powders. Using ADHP phase transition was caused in pure hydroxyapatite structure and apatite and whitlockite were observed as secondary phases. Their particle size, surface area determination and morphological structures have been characterized by Zeta-Sizer, biomimetic hydroxyapatite the BrunauerEmmettTeller analysis and scanning electron microscopy images, respectively. As a result dierent starting materials have aected the structure, particle size and morphological properties of biomimetic hydroxyapatite.

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Micromechanical properties of single crystal hydroxyapatite by nanoindentation

Cited by 131 publications

References 36 publications

The Functional Response of Mesenchymal Stem Cells to Electron‐Beam Patterned Elastomeric Surfaces Presenting Micrometer to Nanoscale Heterogeneous Rigidity

The Functional Response of Mesenchymal Stem Cells to Electron‐Beam Patterned Elastomeric Surfaces Presenting Micrometer to Nanoscale Heterogeneous Rigidity

Understanding the structure–property relationship in cortical bone to design a biomimetic composite

Effect of Different Phosphorus Precursors on Biomimetic Hydroxyapatite Powder Properties

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