MicroMI: A portable microbiological mobile incubator that uses inexpensive lithium power banks for field microbiology

Bizley, Samuel C.; Ray, Partha Pratim; Edwards, Alexander D.

doi:10.1016/j.ohx.2021.e00242

Cited by 9 publications

(7 citation statements)

References 8 publications

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“…Whilst this may not provide the same standardized measure of antibiotic susceptibility of each individual strain present in the sample, it will be important to continue researching the link between "mixed MIC" found from direct susceptibility testing Our assay is simpler than many microfluidic devices, and the open source 3D-printed frame design is flexible and customizable. The system is compact, allowing mobile operation especially if combined with other portable microbiology tools such as mobile incubators [32]. Three-dimensional (3D) printing has become cheaper; we used 3D printers costing £200-£750 (Creality Ender 3 and Prusa i3 MK3) with the materials cost per frame dip slide significantly under £2 to determine simultaneously Gram-negative and Gram-positive bacterial identification plus two different antibiotics.…”

Section: Discussionmentioning

confidence: 99%

“…The dip frame is easy to use and can be easily transport to the field, with no extensive training or introduction to the user required. Combing the dip frame with our previously developed mobile incubator [32] will increase the portability of these tests for broader application, and it may reduce the turnaround time for samples taken a long way from microbiology testing labs. Finally, direct testing with 3D-printed dip slides may contribute to an alternative and rapid way to measure mixed microbes including the microbiome alongside pathogens and measure the antibiotic resistance profiles associated with complex mixtures.…”

Section: Discussionmentioning

confidence: 99%

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3D-Printed Dip Slides Miniaturize Bacterial Identification and Antibiotic Susceptibility Tests Allowing Direct Mastitis Sample Analysis

2022

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The early detection of antimicrobial resistance remains an essential step in the selection and optimization of antibiotic treatments. Phenotypic antibiotic susceptibility testing including the measurement of minimum inhibitory concentration (MIC) remains critical for surveillance and diagnostic testing. Limitations to current testing methods include bulky labware and laborious methods. Furthermore, the requirement of a single strain of bacteria to be isolated from samples prior to antibiotic susceptibility testing delays results. The mixture of bacteria present in a sample may also have an altered resistance profile to the individual strains, and so measuring the susceptibility of the mixtures of organisms found in some samples may be desirable. To enable simultaneous MIC and bacterial species detection in a simple and rapid miniaturized format, a 3D-printed frame was designed for a multi-sample millifluidic dip-slide device that combines panels of identification culture media with a range of antibiotics (Ampicillin, Amoxicillin, Amikacin, Ceftazidime, Cefotaxime, Ofloxacin, Oxytetracycline, Streptomycin, Gentamycin and Imipenem) diluted in Muëller–Hinton Agar. Our proof-of-concept evaluation confirmed that the direct detection of more than one bacterium parallel to measuring MIC in samples is possible, which is validated using reference strains E. coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 10145, and Staphylococcus aureus ATCC 12600 and with mastitis milk samples collected from Reading University Farm. When mixtures were tested, a MIC value was obtained that reflected the most resistant organism present (i.e., highest MIC), suggesting it may be possible to estimate a minimum effective antibiotic concentration for mixtures directly from samples containing multiple pathogens. We conclude that this simple miniaturized approach to the rapid simultaneous identification and antibiotic susceptibility testing may be suitable for directly testing agricultural samples, which is achieved through shrinking conventional tests into a simple “dip-and-incubate” device that can be 3D printed anywhere.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

3D-Printed Dip Slides Miniaturize Bacterial Identification and Antibiotic Susceptibility Tests Allowing Direct Mastitis Sample Analysis

2022

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“…† Increasingly, more designs for portable and affordable incubation for field microbiology have been developed. [52][53][54] Since the mcAST devices are low profile they can be effectively heated from below, without needing a large incubator volume. The incubation stage was prototyped using a heated bed designed for 3D printers, comprising a simple resistive heating element on a printed circuit board (PCB).…”

Section: Direct Poc-mcast Conceptmentioning

confidence: 99%

Moving microcapillary antibiotic susceptibility testing (mcAST) towards the clinic: unravelling kinetics of detection of uropathogenic E. coli, mass-manufacturing and usability for detection of urinary tract infections in human urine

et al. 2023

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“…growth media) and grew the bacteria (for the motility studies); moreover, other materials (culture tubes, L-spreaders, paper disks) are purchased pre-sterilized. W ith mo re tim e, participants could harness lo wcost hardware for sterilization (e.g., pressure-cooker-based autoclaves (Tao et al ( 2012)), incubation and agitation (Diep et al (2021), Arumugam et al ( 2021)).…”

Section: Physics Of Living Systemsmentioning

confidence: 99%

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et al. 2023

Annu. Rev. Fluid Mech.

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Hands-On Research in Complex Systems Schools provide an example of how graduate students and young faculty working in resource-constrained environments can apply key mindsets and methods of tabletop experiments to problems at the frontiers of science. Each day during the Schools’ two-week program, participants work in small groups with experienced tabletop scientists in interactive laboratories on topics drawn from diverse disciplines in science and technology. Using modern low-cost tools, participants run experiments and perform associated data analysis together with mathematical and computational modeling. Participants also engage in other scientific professional activities; in particular, they learn best practices for communicating their results visually, orally, and in writing. In this way, the Hands-On Schools foster the development of scientific leaders in low- and middle-income countries. Expected final online publication date for the Annual Review of Fluid Mechanics, Volume 55 is January 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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MicroMI: A portable microbiological mobile incubator that uses inexpensive lithium power banks for field microbiology

Cited by 9 publications

References 8 publications

3D-Printed Dip Slides Miniaturize Bacterial Identification and Antibiotic Susceptibility Tests Allowing Direct Mastitis Sample Analysis

3D-Printed Dip Slides Miniaturize Bacterial Identification and Antibiotic Susceptibility Tests Allowing Direct Mastitis Sample Analysis

Moving microcapillary antibiotic susceptibility testing (mcAST) towards the clinic: unravelling kinetics of detection of uropathogenic E. coli, mass-manufacturing and usability for detection of urinary tract infections in human urine

Advancing Access to Cutting-Edge Tabletop Science

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