Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora

Gao, Ruize; Liu, Chongxi; Zhao, Junwei; Jia, Feiyu; Yu, Chao; Yang, Lingyu; Wang, Xiangjing; Xiang, Wensheng

doi:10.1007/s10482-013-0074-3

Cited by 159 publications

(64 citation statements)

References 37 publications

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“…The recent description of a novel species of Micromonospora (i.e. M. jinlongensis) also proposed to emend the genus [14]. Undoubtedly, the observations shown here also leave further room for improvement on the taxonomic properties of the genus not only from a biotechnological perspective but also from an ecological one.…”

Section: Introductionmentioning

confidence: 58%

Unexpected Properties of Micromonosporae from Marine Origin

Maldonado¹,

Quintana²

2015

AiM

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Members of the genus Micromonospora show a complex life cycle which normally involves the presence of substrate or vegetative mycelia and sporulation with single spores born on the vegetative hyphae followed by the synthesis of a dark extracellular polysaccharide. Bergey's Manual states that micromonosporae rarely produces aerial mycelia (AM) and if so, is considered "sterile". During the characterisation of novel micromonosporae from the Sea of Cortes, it was observed that AM is produced reproducibly in the presence of certain carbon and/or nitrogen sources. Micromanipulation of the AM subcultured onto fresh media produced colonies; hence, this structure should not be called "sterile". TEM of the AM producing isolates suggests that the spores also show activity as reported for bacilli of marine origin. This would be the first report of the presence of "inducible" AM in micromonosporae of marine sources and that the spores of this genus have a role other than just dispersal. KeywordsActinobacteria, Actinomycetes, Aerial Mycelia, Micromonospora, SporesThe genus Micromonospora is the type genus of the family Micromonosporaceae and, after Streptomyces, is still regarded as a good source of bioactive compounds [1] [2]. Among the Actinobacteria, that is Gram Positive bacteria with a high GC content, Micromonospora are ubiquitous in aquatic environments but their distribution and specific role is poorly understood. Because of their economic importance, the best studied life cycle is that of the genus Streptomyces. In streptomycetes, a single spore will germinate and give rise to a colony which will * Corresponding author. L. A. Maldonado, E. T. Quintana 453 produce two but related complex structures: (a) the substrate or vegetative and (b) the aerial mycelia (AM), the latter which will undergo further differentiation into spores [3]. For the genus Micromonospora, there is only one report dealing with the formation of AM [4] but taxonomic references mention this as "rare" and even "sterile", notably because the "single spores" will be attached to the substrate or vegetative mycelia [5]. Description of species within Micromonospora is usually based on a polyphasic approach employing chemotaxonomic markers, geno-and phenoypic properties but this is usually cumbersome because of the limited and taxonomic value of the physiological properties of micromonosporae. Micromonospora colonies will start as yellow or light orange, then become darkorange and end with a black mucoid exopolysaccharide which completely covers the colony and indicates sporulation [6]. In this work, micromonosporae recovered from sediments of the Sea of Cortes (aka Gulf of California; [7]) were characterised using a polyphasic taxonomic approach including both geno-and phenotypic properties. During the phenotypical characterization of the isolates growing on the International Streptomyces Project media (ISP; 30 carbon sources and 15 nitrogen sources; [8]) it was noticed that the strains reproducibly developed AM on media supplemented with severa...

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Section: Introductionmentioning

confidence: 58%

Unexpected Properties of Micromonosporae from Marine Origin

Maldonado¹,

Quintana²

2015

AiM

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“…To determine cellular fatty acid compositions, strain NEAU-G9 T and the type strains of S. yanglinensis and S. misakiensis were cultivated in ISP 2 medium in shake flasks at 28 8C for 14 days. Fatty acid methyl esters were extracted from the biomass as described by Gao et al (2014) and analysed by GC-MS using the method of Xiang et al (2011).…”

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confidence: 99%

Streptomyces polygonati sp. nov., an endophytic actinomycete isolated from a root of Polygonatum odoratum (Mill.)

Guo

Liu

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A novel actinomycete, designated strain NEAU-G9 T , was isolated from the root of Polygonatum odoratum (Mill.) collected from Harbin, Heilongjiang province, north China, and was characterized using a polyphasic approach. Key morphological and chemotaxonomic properties confirmed the affiliation of strain NEAU-G9 T to the genus Streptomyces. Strain NEAU-G9 T contained LL-diaminopimelic acid as the diamino acid. The predominant menaquinones were MK-9(H 8 ), MK-9(H 6 ) and MK-9(H 4 ). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant fatty acids were iso-C 16 : 0 , anteiso-C 15 : 0 and C 16 : 0 .16S rRNA gene sequence similarity studies showed that strain NEAU-G9 T belongs to the genus Streptomyces and exhibits the highest sequence similarity to Streptomyces yanglinensis JCM 13275 T (97.75 %). However, phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-G9 T is most closely related to Streptomyces misakiensis JCM 4062 T (97.12 % sequence similarity). A combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-G9 T can be clearly distinguished from S. yanglinensis JCM 13275 T and S. misakiensis JCM 4062 T . Consequently, strain NEAU-G9 T represents a novel species of the genus Streptomyces, for which the name Streptomyces polygonati sp. nov. is proposed. The type strain is NEAU-G9 T (5CGMCC 4.7237 T 5DSM 100521 T ).

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“…To determine cellular fatty acid compositions, strain 1H-HV4 T was cultivated in ISP 2 for 7 days in shake flasks at 28 8C. Fatty acid methyl esters were extracted from the biomass as described by Gao et al (2014) and analysed by GC-MS using the method of Xiang et al (2011).…”

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confidence: 99%