Micromonospora rosaria sp. nov., nom. rev., the Rosaramicin Producer

Abstract

The name of the produter of the macrolide antibiotic rosaramicin, Micromonospora rosaria NRRL 3718* (T = type strain), was not included on the Approved Lists of Bacterial Names because the original publication did not contailr a full description of this strain. Following its first appearance in the literature and its subsequent availability, M . rosaria NRRL 3718 frequently appeared in studies as a comparative organism when new antibiotic-producing species of Micrornonsporu were described. Therefore, we formally revive the name M . rosaria, present a full species description, and designate strain NRRL 3718 as the type strain.Wagman et al. (23) described a new macrolide antibiotic produced by an organism designated Micromonospora rosaria. Although the producing culture was named and deposited in the Northern Utilization and Research Division, U.S. Department of Agriculture, Peoria, Ill., as strain NRRL 371gT (T = type strain), a full description of the strain was not given. Therefore, the species was not included on the Approved Lists of Bacterial Names (19).Since that initial publication, M . rosaria NRRL 37MT has been used as a reference strain in numerous studies describing new species of antibiotic-producing micromonosporae (4, 7, 11, 17, 24). In an attempt to provide continuity in the literature, we propose to revive the name Micromonospora rosaria in accordance with Rule 28a and Provisional Rules B2 and B3 of the International Code of Nomenclature of Bacteria (9).Strains. Since the initial discovery of rosaramicin, we have isolated rosaramicin-producing strains from soil, and our co-workers have generated mutants, including strains blocked in the production of rosaramicin (5,22). The species description given below is based on a taxonomic analysis of strain NRRL 37BT and six of these strains ( Table 1).Procedures described by Horan and Brodsky (8) were used for maintenance of strains and preparation of inocula. The observation and characterization of micromonospora were incubated for 14 to 21 days at 30°C. Each color designation assigned to the vegetative mycelial pigments consisted of a color name (21) and a color chip number (3).Carbon utilization was determined by the procedures of Luedemann and Brodsky (15) The species description is given below. Micromonospora rosaria sp. nov., nom. rev. (ex Wagman, Waitz, Marquez, Murawski, Oden, Testa, and Weinstein, 1972). The specific epithet refers to the wine red diffusible pigment formed by strain NRRL 37NT.The vegetative hyphae are branched and approximately 0.5 pm in diameter. Spores that are 1.5 to 1.8 km in diameter occur along the length of the hyphae, are borne singly, and are either sessile or on short sporophores. Growth and sporulatioh is of the open web type of Luedemann (13) (Fig.

The Occurrence of Bioactive Micromonosporae in Aquatic Habitats of the Sunshine Coast in Australia

“…Other researchers have reported a correlation between self-produced antibiotic resistance and the antibiotic production [17, 29, 31]. Consequently, gentamicin resistance has been used in rapid screening strategies for the isolation of antibiotic producing micromonosporae [5, 8, 31].…”

Modestobacter multiseptatus gen. nov., sp. nov., a budding actinomycete from soils of the Asgard Range (Transantarctic Mountains).

“…DNase, lipase and phosphatase activities were tested as described by Smibert & Krieg (1981). Degradation of xylan, arbutin, aesculin, adenine, hypoxanthine and xanthine was studied according to Horan & Brodsky (1986). The following enzyme activities were tested as described in the literature : catalase (Smibert & Krieg, 1981), cytochrome oxidase (Deibel & Evans, 1960), sialidase (Potier et al, 1979), and type II restriction endonuclease (Whitehead & Brown, 1985).…”

Micromonospora eburnea sp. nov., isolated from a Thai peat swamp forest

“…Skerman et al (1980) listed 12 species and seven subspecies as members of the genus Micromonospora; subsequently, Micromonospora olivasterospora (Kawamoto et al, 1983), Micromonospora rosaria (Horan & Brodsky, 1986) and Micromonospora chersina (Tomita et al, 1992) have been recognized as novel species. Lee et al (1999) transferred Catellatospora matsumotoense to the genus Micromonospora as Micromonospora matsumotoense on the basis of 16S rRNA gene sequence analysis.…”