Micronucleus Assay-based Evaluation of Radiosensitivity of Lymphocytes among Inhabitants Living in High Background Radiation Area of Mamuju, West Sulawesi, Indonesia

Syaifudin, Mukh; Defiyandra, Vira Putri; Nurhayati, Siti; Purnami, Sofiati; Pudjadi, Eko

doi:10.4103/genint.genint_2_18

Cited by 8 publications

(7 citation statements)

References 25 publications

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“…Other assessments such as proteomic with mass spectrometry were conducted to determine the research on γ-h2ax, g0, and g2 micronucleus assays. This is in addition to tp53 arg72pro genotyping, nuclear division, mitotic indexes, etc (16) Based on all parameters or biomarkers evaluation and assessment, the natural radiation exposure in MAMUJU failed significantly to affect the health condition of the population under study. Furthermore, there is no significant difference between the study group and its control, which suggests a low risk of natural radiation-exposed related to the induction of any negative impacts (13)(14)(15)(16).…”

Section: Natural and Man-made Radioactivitymentioning

confidence: 97%

“…This is in addition to tp53 arg72pro genotyping, nuclear division, mitotic indexes, etc (16) Based on all parameters or biomarkers evaluation and assessment, the natural radiation exposure in MAMUJU failed significantly to affect the health condition of the population under study. Furthermore, there is no significant difference between the study group and its control, which suggests a low risk of natural radiation-exposed related to the induction of any negative impacts (13)(14)(15)(16). Therefore, a research to protect the affected inhabitants of such areas with improved methodologies is important and urgently needed.…”

Section: Natural and Man-made Radioactivitymentioning

confidence: 97%

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Safety Assessments for Supporting the Application of Nuclear Technology in Indonesia and a Comprehensive Study on High Natural Radioactivity Area

Ikram

Jumadi²,

Nurokhim³

et al. 2021

jfn

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historis, BATAN didirikan untuk mengantisipasi dampak negatif akibat percobaan ledakan nuklir di wilayah Pasifik di masa lalu. Saat ini, satu Pusat dari lembaga pemerintah ini memiliki tugas untuk melakukan penelitian dan pengembangan serta layanan di bidang keselamatan dan metrologi radiasi. Tujuan dari makalah ini adalah membahas semua kegiatan yang dilakukan oleh Pusat ini yang mencakup banyak aspek dalam pengkajian keselamatan pekerja, masyarakat dan lingkungan. Untuk metrologi radiasi, sumber standar laboratorium dosimetri (SSDL) dalam layanan kalibrasi dibangun untuk mendukung keberhasilan dalam radioterapi, di mana pencapaian tertinggi adalah sebagai Lembaga yang Ditunjuk untuk Radiasi Pengion. Penelitian kesehatan mencakup pemanfaatan sinar gamma untuk mengembangkan bahan vaksin, diagnosis penyakit metabolik dengan teknik kedokteran nuklir dan mikrodosimetri dalam penilaian radiodiagnostik. Untuk radioekologi dan biologi radiasi, salah satu penelitian utama adalah penentuan dosis efektif dan studi epidemiologi di Mamuju sebagai daerah radiasi latar belakang tinggi (HBRA) yang memberikan kesempatan unik untuk mempelajari efek kesehatan dari paparan radiasi kronis tingkat rendah pada manusia. Melalui kerja sama dengan Universitas Hirosaki, penting untuk menilai kesehatan dan status fisik serta kerusakan asam sitogenetik dan deoksinukleotida (DNA) dalam limfosit populasi lokal yang dapat memberikan informasi yang berharga. Studi dosimetri dilakukan dengan pengukuran langsung di area lapangan, termasuk analisis paparan radiasi eksternal dari tingkat dosis gamma di dalam dan di luar ruangan. Penentuan radiasi internal dilakukan dengan mengambil sampel makanan, urin, air minum, dan air inhalasi (radon). Studi epidemiologis melalui respon sitogenetik dari tempat tinggal yang didukung oleh γ-H2AX, G0 dan G2 micronucleus, dan genotip TP53 dari daerah penelitian menunjukkan bahwa tidak ada perbedaan yang signifikan dalam efek radiasi alami dengan kontrol. Studi radiologi kelautan dan pemantauan radionuklida di seluruh kepulauan Indonesia juga tidak menunjukkan suatu dampak negatif. Praktik kedaruratan nuklir pun juga harus dilakukan secara rutin di Pusat ini untuk memperkuat budaya keselamatan dan keamanan.

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Section: Natural and Man-made Radioactivitymentioning

confidence: 97%

Section: Natural and Man-made Radioactivitymentioning

confidence: 97%

Safety Assessments for Supporting the Application of Nuclear Technology in Indonesia and a Comprehensive Study on High Natural Radioactivity Area

Ikram

Jumadi²,

Nurokhim³

et al. 2021

jfn

View full text Add to dashboard Cite

show abstract

“…To our knowledge, there are no other specific studies indicating the presence of an AR in patients exposed to ionizing radiation for medical reasons. Some studies on AR in humans have only been performed ex vivo on lymphocytes taken from healthy donors [ 10 ] or refer to cohorts of healthy donors from occupational or environmental exposures [ 11 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Changes in Radiosensitivity to Gamma-Rays of Lymphocytes from Hyperthyroid Patients Treated with I-131

Dini

Salvatori

Belli³

et al. 2022

IJMS

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This study investigated the peripheral blood lymphocytes (PBL) response to a dose of γ-rays in patients treated with radioiodine (I-131) for hyperthyroidism vs. healthy controls, to gain information about the individual lymphocytes’ radio-sensitivity. Blood samples were taken from 18 patients and 10 healthy donors. Phosphorylated histone variant H2AX (γ-H2AX) and micronuclei (MN) induction were used to determine the change in PBL radio-sensitivity and the correlations between the two types of damage. The two assays showed large inter-individual variability in PBL background damage and in radio-sensitivity (patients vs. healthy donors). In particular, they showed an increased radio-sensitivity in 36% and 33% of patients, decrease in 36% and 44%, respectively. There was a scarce correlation between the two assays and no dependence on age or gender. A significant association was found between high radio-sensitivity conditions and induced hypothyroidism. PBL radio-sensitivity in the patient group was not significantly affected by treatment with I-131, whereas there were significant changes inter-individually. The association found between clinical response and PBL radio-sensitivity suggests that the latter could be used in view of the development of personalized treatments.

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“…These three distinguished endpoints can be analyzed at the same time in this assay [6]. Their frequencies in peripheral blood lymphocytes are formed due to chromosomal aberrations arising under the action of ionizing radiation with a similar formation mechanism [13], [14]. These nuclear anomalies are associated with cancer risk.…”

Section: Introductionmentioning

confidence: 99%

The Evaluation of Frequencies of Cytogenetic Biomarkers in Lymphocyte of Residents from High Natural Radiation Area in West Sulawesi, Indonesia

Syaifudin¹,

Ramadhani²,

Purnami³

et al. 2022

International Journal on Advanced Science, Engineering and Information Technology

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Residents living in high natural radiation areas may pose health consequences such as cancer. The study aims to evaluate the frequencies of cytogenetic biomarkers covering micronuclei (MN), nucleoplasmic bridge (NPB), and nuclear bud (NBUD) with the cytokinesis-block micronucleus (CBMN) assay. This cross-sectional study was done on 51 blood lymphocytes from the resident of Mamuju, West Sulawesi, Indonesia that was done according to standard procedure. After being stained with Giemsa solution, these biomarkers were observed on about 1,000 binucleated cells. The results showed a low frequency of MN (0.0162 in 36,091 cells) and extremely low frequencies of other biomarkers (0.00019 and 0.00061 for NPB and NBUD, respectively) in the study area, whereas these were 0.0225 MNs in 15,000 cells, and 0.00013 and 0.00120 for NPB and NBUD, respectively, in the control area. MNs and NBUDs were lower in the study area compared to control. No statistically significant differences (p>0.05) in NPB were found between the two areas, but not for NBUD. The frequencies of MN and NPB in female is higher than that of male in both areas. In the control group, males experienced a decrease in the number of NPB to 0.7 times compared to females, and every extra one year of age, 1.047 times more NBUDs were found. None of the confounding factors was influenced in the study group. It was concluded that there is no impact of high natural radiation to the local residents based on cytogenetics evaluation, with a note that further studies on a higher number of samples and other relevant biomarkers are required.

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Micronucleus Assay-based Evaluation of Radiosensitivity of Lymphocytes among Inhabitants Living in High Background Radiation Area of Mamuju, West Sulawesi, Indonesia

Cited by 8 publications

References 25 publications

Safety Assessments for Supporting the Application of Nuclear Technology in Indonesia and a Comprehensive Study on High Natural Radioactivity Area

Safety Assessments for Supporting the Application of Nuclear Technology in Indonesia and a Comprehensive Study on High Natural Radioactivity Area

Changes in Radiosensitivity to Gamma-Rays of Lymphocytes from Hyperthyroid Patients Treated with I-131

The Evaluation of Frequencies of Cytogenetic Biomarkers in Lymphocyte of Residents from High Natural Radiation Area in West Sulawesi, Indonesia

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