Micronucleus frequency among Iraqi thyroid disorder patients

Abstract

Micronucleus (MN) assay has been extensively used in detection of DNA damage, instability in cancer, and genetic disorders. In the current study, MN, binucleated cells, and nuclear division index (NDI) were investigated in Iraqi patients with thyroid disorders. The results indicated significantly (p < 0.05) increased binucleated cells with micronucleus (BNMN) frequencies in thyroid cancer group (37.58 ± 3.07) versus other thyroid disorder groups (6.60 ± 1.29, 14.90 ± 1.69, 15.56 ± 1.76). On the other hand, the frequency of micronucleus per 1,000 and the NDI were significantly (p < 0.05) decreased in hypothyroidism (MN 1.55 ± 0.36) (NDI 0.009 ± 0.001) versus other thyroid disorder groups (MN: 6.05 ± 0.97, 6.09 ± 0.53, 5.34 ± 0.56) (NDI: 0.049 ± 0.003, 0.032 ± 0.002, 0.025 ± 0.002), with no difference versus healthy group (0.0 ± 0.0). The number of BNMN and MN are parallel to the severity of thyroid disorders which were 6.60 ± 1.29, 14.90 ± 1.69, 15.56 ± 1.76, and 37.58 ± 3.07 for hypothyroidism, thyroid toxic goiter, thyroid nontoxic goiter, and thyroid cancer, respectively. The number of BNMN and MN are parallel to the severity of thyroid disorders which were 6.60 ± 1.29, 14.90 ± 1.69, 15.56 ± 1.76, and 37.58 ± 3.07 for hypothyroidism, thyroid toxic goiter, thyroid nontoxic goiter, and thyroid cancer, respectively. The results also indicate that there were no significant differences among age and sex groups as related with BNMN formation within each thyroid disorder groups and healthy control group.

Micronucleus, nucleoplasmic bridge and nuclear bud frequencies in patients with laryngeal carcinoma

“…However, there are many studies on genomic damage in different cancer types. The result on the MN frequency obtained in the present study is consistent with the results of other studies 8,[12][13][14][15] . There are only a few studies evaluating the frequency of NPB, NBUD and MN 21 .…”

“…These nuclear anomalies, which are markers of genomic instability, are frequently seen in cancer 6 . Numerous studies have validated that there is a positive correlation between increased MN, NPB and NBUD frequencies in PBL and cancer risk 6,8,[12][13][14][15] . The CBMN assay is a useful method to monitor genomic instability, and many recent events reveal that the increased genomic instability in PBL might be an early event in carcinogenesis 6,16 .…”

Cytogenetic abnormalities in lymphocytes evaluated with micronucleus assay in medical personnel occupationally exposed to ionizing radiation

“…In this study the authors confirmed that two men in the exposed group, who have hypothyroidism (receive a therapy with euthyrox), have increased number of MNi (Table 2). In the research of AL FAISAL et al (2012), there was an evidence of increased MN frequencies in patients with thyroid disorder, compared to healthy population. So many factors like age, gender, smoking etc.…”

Detection of five substitution TPO mutations in Polycystic Ovary Syndrome (PCOS) and thyroid hormones disturbance patients

“…The inadequate thyroid hormones availability due to defect of synthesis of these hormones, the majority of these defects are due to mutations in thyroglobulin-TG and thyroid peroxidase-TPO genes [12]. Mutations among TPO and TG genes associated with thyroid disorders were strongly proved [13,14,15]. The current study is attempted to find the TPO mutations associated with PCOS combined with thyroid hormones disturbances.…”