The microorganisms present in the masks of the inhabitants of Pucallpa for the protection of COVID-19 were determined by collecting surgical masks, KN95 and cloth, used by the inhabitants from the districts of Campoverde, Yarinacocha, Manantay and Callería, obtaining 15 masks per district. The identification of mycoorganisms was carried out through biochemical (traditional method) and automated (Vitex) tests. Twelve bacterial species were isolated (Eneterobacter cloacae ssp cloacae, Entererobacter aerogenes, Escherichia coli, Klebsiella pneumoniae ssp pneunoniae, Raoltella ornithinolytica, Kocuria kristinae, Acinetobacter baumannii, Acinetobacter cacoacetocus, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Pseudomonas putidaand Staphylococcus epidermidis) distributed in 6 families as Eneterobateriaceae. Microcococcaceae, Moraxellaceae, Morganellaceae, Pseudomonadaceae and Staphylococcaceae. In the case of fungi, two families Trichocomaceae (Aspergillus spp) and Saccharomycetaceae (Candida famataand Candida parapsiliosis) were found. It was not possible to find the COVID-19 virus in the masks, since there was no evidence of the presence of the N gene, ORF1ab gene. The highest number of bacterial species corresponds to the Enterobacteriaceae family, recorded in the district of Campoverde and Yarinacocha, while Aspergillus spp. were recorded in the four districts.