Microorganisms in metalworking fluids: Current issues in research and management

Trafny, Elżbieta Anna

doi:10.2478/s13382-013-0075-5

Cited by 39 publications

(38 citation statements)

References 47 publications

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“…One issue that has been getting more attention is the contamination of microorganisms and the generation of endotoxin during the use of water-soluble MWFs. A wide variety of microorganisms have been found where the most commonly-reported microorganisms are aerobic Gram-negative bacteria such as Pseudomonas [78]. Biocides are added to control its growth but are not always sufficiently effective.…”

Section: Occupational Exposure To Metalworking Fluidsmentioning

confidence: 99%

Upper Airway Mucosal Inflammation : Proteomic Studies after Exposure to Irritants and Microbial Agents

Fornander¹

2015

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People are, in their daily lives, exposed to a number of airborne foreign compounds that do not normally affect the body. However, depending on the nature of these compounds, dose and duration of exposure, various airway symptoms may arise. Early symptoms are often manifested as upper airway mucosal inflammation which generates changes in protein composition in the airway lining fluid.This thesis aims at identifying, understanding mechanisms and characterizing protein alterations in the upper airway mucosa that can be used as potential new biomarkers for inflammation in the mucosa. The protein composition in the mucosa was studied by sampling of nasal lavage fluid that was further analyzed with a proteomic approach using two-dimensional gel electrophoresis and mass spectrometry. Additionally, by studying factors on site through environmental examination, health questionnaires and biological analyses, we have tried to understand the background to these protein alterations and their impact on health.Respiratory symptoms from the upper airways are common among people who are exposed to irritative and microbial agents. This thesis have focused on personnel in swimming pool facilities exposed to trichloramine, metal industry workers exposed to metalworking fluids, employees working in damp and moldy buildings and infants diagnosed with respiratory syncytial virus infection. The common denominator in these four studies is that the subjects experience upper airway mucosal inflammation, which is manifested as cough, rhinitis, phlegm etc. In the three occupational studies, the symptoms were work related. Notably, a high prevalence of perceived mucosal symptoms was shown despite the relatively low levels of airborne irritants revealed by the environmental examination. Protein profiling verified an ongoing inflammatory response by identification of several proteins that displayed altered levels. Interestingly, innate immune proteins dominated and four protein alterations occurred in most of the studies; SPLUNC1, protein S100A8 and S100A9 and alpha-1-antitrypsin. Similarly, these proteins were also found in nasal fluid from children with virus infection and in addition a truncated form of SPLUNC1 and two other S100 proteins (S100A7-like 2 and S100A16), not previously found in nasal secretion, were identified.Altogether, the results indicate the potential use of a proteomic approach for identifying new biomarkers for the upper respiratory tract at an early stage in the disease process after exposure to irritant and microbial agents. The results indicate an effect on the innate immunity system and the proteins; SPLUNC1, protein S100A8 and S100A9 and alpha-1-antitrypsin are especially promising new biomarkers. Moreover, further studies of these proteins may help us to understand the molecular mechanisms involved in irritant-induced airway inflammation. POPULÄRVETENSKAPLIG SAMMANFATTNING FÖRÄNDRAD PROTEINSAMMANSÄTTNING I DE ÖVRE LUFTVÄGARNA EFTER EXPONERING FÖR SLEMHINNEIRRITERANDE ÄMNENVarje dag utsätts vi för partikla...

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Section: Occupational Exposure To Metalworking Fluidsmentioning

confidence: 99%

Upper Airway Mucosal Inflammation : Proteomic Studies after Exposure to Irritants and Microbial Agents

Fornander¹

2015

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“…Clean machines use MWFs more economically and extend fluid life. Any dirt and oil allowed to remain in the system simply recirculates, resulting in plugged coolant lines, unsightly machine buildup and bacterial growth (9). Before loading the system with the fresh fluid, it is necessary to clean the whole system (3) in order to keep biological growth in check and maintain proper system operation.…”

Section: Introductionmentioning

confidence: 99%

“…The microbial contamination of water miscible metalworking fluids (MWFs) is a serious problem in metal industry. A good maintenance of MWF re-circulation systems can extend the lifetime of coolants and ensure the quality of the machined parts (9).…”

Section: Introductionmentioning

confidence: 99%

Real Time Monitoring and Automatic Regulation System for Metalworking Fluids

Gerulová

Neštický

Buranská

et al. 2016

Research Papers Faculty of Materials Science and Technology Slovak University of Technology

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“…Apart from oil-derived xenobiotics they contain a number of additives including biocides, heavy metals, surfactants and many other agents [3,11]. In addition, cutting fluids are often contaminated by various bacterial (both gram-negative and gram-positive) and fungal strains (for detailed information see the review articles [12][13][14][15][16]). Such indigenous microbial strains may be beneficial for their tolerance towards toxic chemical constituents; however, they are often pathogenic and can cause adverse effects on human health, especially on the respiratory system and skin [3,[17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Bioremediation Of A Spent Metalworking Fluid With Auto- And Allochthonous Bacterial Consortia

Kaszycki

Petryszak

Supel

2015

Ecological Chemistry and Engineering S

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Spent mineral oil-based metalworking fluids are waste products of the machining processes and contribute substantially to the global industrial pollution with petroleum oil products. Wastewaters containing oily emulsions are ecologically hazardous and thus a variety of methods have been implemented to prevent these effluents from affecting the natural environment. Most of these methods rely upon physical-chemical treatment and phase separation; however, none of them proved to be effective enough to meet tightening environmental regulations. Therefore, novel technologies need to be elaborated and there is growing interest in implementing biological treatment methods based on microbial bioremediation. In this study an oil/water emulsion obtained from a waste stream of the metal-processing industry was tested for biodegradability of its organic constituents. This liquid waste was found non-toxic to bacterial consortia and was colonized with indigenous microorganisms (approx. 10 7 cfu · cm -3). The total load of organic content was determined as a chemical oxygen demand (COD) value of 48 200 mg O2 · dm -3. Emulsion treatment was carried out using a threefold wastewater dilution and employing two variants of biostimulated aerobic bacterial communities: (1) uninoculated emulsion, where bioremediation was carried out by the autochthonous bacteria alone, and (2) wastewater samples inoculated with a ZB-01 microbial consortium which served as a source of specialized bacteria for process bioaugmentation. Biodegradation efficiency achieved in a 14-day test was monitored by measuring both the COD parameter and the concentration of high-boiling organic compounds. Both approaches yielded satisfactory results showing significant reduction of the emulsion organic fraction; however, the resultant decrease of wastewater load tended to be more efficient for the case where the process was bioaugmented with the inoculated consortium. Gas chromatography analyses coupled with mass spectrometric detection (GC-MS) confirmed high degradation yields obtained for both cases studied (58 and 71%, respectively) in a 28-day test. It is concluded that oil-based metalworking emulsions can undergo efficient biological treatment under conditions enabling aerobic bacterial proliferation and that xenobiotic biodegradation kinetics can be accelerated by bioaugmenting the process with allochthonous microbial consortia.

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Microorganisms in metalworking fluids: Current issues in research and management

Cited by 39 publications

References 47 publications

Upper Airway Mucosal Inflammation : Proteomic Studies after Exposure to Irritants and Microbial Agents

Upper Airway Mucosal Inflammation : Proteomic Studies after Exposure to Irritants and Microbial Agents

Real Time Monitoring and Automatic Regulation System for Metalworking Fluids

Bioremediation Of A Spent Metalworking Fluid With Auto- And Allochthonous Bacterial Consortia

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